[Histonet] RE: Wrinkles in whole bone mouse tibias
Dear Nancy, Just wanted to add that you should face into the block before the soak in the water bath and transfer to ice-water. This method gave me about 15 good sections before needing to repeat the process. Again, best of luck! Leisha Machin Aab Cardiovascular Research Institute University of Rochester "The capacity to blunder slightly is the real marvel of DNA. Without this special attribute, we would still be anaerobic bacteria and there would be no music." ~Lewis Thomas From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Saturday, April 14, 2012 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 101, Issue 19 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Wrinkles in whole bone mouse tibias (Machin, Leisha E) 2. RE: New Reagent Lot Verification (Rathborne, Toni) 3. RE: Wrinkles in whole bone mouse tibias (Meghana Bapardekar) 4. "know error system" prostate biopsies (Robert Jann) 5. RE: "know error system" - prostate biopsies (Cynthia Pyse) 6. Unsubscribtion. (Kaz) 7. Diane Tokugawa/CA/KAIPERM is out of the office. (diane.tokug...@kp.org) 8. RE: New Reagent Lot Verification (tahs...@brain.net.pk) -- Message: 1 Date: Fri, 13 Apr 2012 14:24:46 -0400 From: "Machin, Leisha E" Subject: [Histonet] Re: Wrinkles in whole bone mouse tibias To: "histonet@lists.utsouthwestern.edu" Message-ID: <7c06700a9c2da240a61046b23f5e84d2303e7...@urmcms3.urmc-sh.rochester.edu> Content-Type: text/plain; charset="us-ascii" Dear Nancy, I had your mouse tibia problem last year; the marrow consistently folded up accordion-style. I believe the problem was solved by placing the block in the water bath (for less than a minute) then into an ice-water slurry for about 3 minutes. Hope this help - best of luck! Leisha Machin "The capacity to blunder slightly is the real marvel of DNA. Without this special attribute, we would still be anaerobic bacteria and there would be no music." ~Lewis Thomas From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Friday, April 13, 2012 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 101, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. ACMS perspective on Aetna's new requirments. (Nicole Tatum) 2. Prepared STD slides? (Jon Krupp) 3. HistoTALK at the Region III Meeting (David Kemler) 4. Competency assesment forms for Pathologists (Sheila Adey) 5. Re: Prepared STD slides? (Kim Donadio) 6. Re: Prepared STD slides? (Lee & Peggy Wenk) 7. "know error system" - prostate biopsies (Richard Cartun) 8. RE: "know error system" - prostate biopsies (Lester Raff MD) 9. Formalin asfety question (O'Donnell, Bill) 10. Wrinkles in whole bone mouse tibias (nancy lowen) 11. RE: "know error system" - prostate biopsies (Heath, Nancy L.) 12. RE: Formalin asfety question (O'Donnell, Bill) 13. RE: New Reagent Lot Verification (Settembre, Dana) 14. Re: Wrinkles in whole bone mouse tibias (Louise Renton) -- Message: 1 Date: Thu, 12 Apr 2012 13:32:30 -0400 (EDT) From: "Nicole Tatum" Subject: [Histonet] ACMS perspective on Aetna's new requirments. To: histonet@lists.utsouthwestern.edu Message-ID: <2628.208.62.167.196.1334251950.squir...@webmail.realpages.com> C
[Histonet] RE: Wrinkles in whole bone mouse tibias
Yes it should work, placing the block in the water bath (for less than a minute) then into an ice-water slurry for about 3 minutes as we experienced with similar samples. Hope this helps! Meghana V. Bapardekar, Ph. D., Dept. of Histopathology, Cleveland Biolabs, 73 High Street, Buffalo, NY - 14203. Phone: 716-849-6810, ext.: 357 Fax: 716-849-6817 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Machin, Leisha E Sent: Friday, April 13, 2012 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Wrinkles in whole bone mouse tibias Dear Nancy, I had your mouse tibia problem last year; the marrow consistently folded up accordion-style. I believe the problem was solved by placing the block in the water bath (for less than a minute) then into an ice-water slurry for about 3 minutes. Hope this help - best of luck! Leisha Machin This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Wrinkles in whole bone mouse tibias
Dear Nancy, I had your mouse tibia problem last year; the marrow consistently folded up accordion-style. I believe the problem was solved by placing the block in the water bath (for less than a minute) then into an ice-water slurry for about 3 minutes. Hope this help - best of luck! Leisha Machin "The capacity to blunder slightly is the real marvel of DNA. Without this special attribute, we would still be anaerobic bacteria and there would be no music." ~Lewis Thomas From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Friday, April 13, 2012 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 101, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. ACMS perspective on Aetna's new requirments. (Nicole Tatum) 2. Prepared STD slides? (Jon Krupp) 3. HistoTALK at the Region III Meeting (David Kemler) 4. Competency assesment forms for Pathologists (Sheila Adey) 5. Re: Prepared STD slides? (Kim Donadio) 6. Re: Prepared STD slides? (Lee & Peggy Wenk) 7. "know error system" - prostate biopsies (Richard Cartun) 8. RE: "know error system" - prostate biopsies (Lester Raff MD) 9. Formalin asfety question (O'Donnell, Bill) 10. Wrinkles in whole bone mouse tibias (nancy lowen) 11. RE: "know error system" - prostate biopsies (Heath, Nancy L.) 12. RE: Formalin asfety question (O'Donnell, Bill) 13. RE: New Reagent Lot Verification (Settembre, Dana) 14. Re: Wrinkles in whole bone mouse tibias (Louise Renton) -- Message: 1 Date: Thu, 12 Apr 2012 13:32:30 -0400 (EDT) From: "Nicole Tatum" Subject: [Histonet] ACMS perspective on Aetna's new requirments. To: histonet@lists.utsouthwestern.edu Message-ID: <2628.208.62.167.196.1334251950.squir...@webmail.realpages.com> Content-Type: text/plain;charset=iso-8859-1 This is a letter From the American College of Mohs Surgery in reguards to Aetna letter for CAP requiremnet. April 5, 2012 I read your letter of March 23, 2012, outlining Aetna?s new requirement for CLIA and CAP certification for certain pathology services with dismay. I am very concerned these requirements could lead to lower cure rates, increased tissue loss and scarring, and even unnecessary deaths in your patients with problem skin cancer with Aetna insurance. CAP certification will be almost impossible to achieve for small Mohs Micrographic surgery frozen section laboratories. These labs can only be CAP certified if directed by an anatomic pathologist or dermatopathologist. Mohs surgeons, who have extensive additional training in pathology, and training to run their laboratories, do not fit into either niche. Mohs surgeons deal with the most difficult and recurrent skin cancers. Please find attached a copy of the CPT coding assistant from 2008 describing Mohs surgery, and detailing what services are included in it. With your new restrictions, immunohistochemistry (CPT code 88342) cannot be billed with Mohs surgery for malignant melanoma and spindle cell tumors. This will restrict the use of Mart 1 immuno stains and other special cytokeratin stains. This will result in lower cure rates and an increased number of deaths. These stains on additional frozen sections are not part of Mohs surgery, and are appropriately billed for separately. In addition, Mohs surgeons will not be able to bill for the occasional special stains on frozen sections (CPT code 8814) such as oil red O on a sebaceous carcinoma, or toluidine blue (in addition to the hematoxylin and Eosin stained frozen sections) to clarify an area of inflammation. Performing these special stains on ambiguous frozen sections often saves the patient additional surgery and tissue loss, and saves Aetna money, because another stage of Mohs surgery is avoided. These additional stains are not part of the Mohs surgery and are appropriately billed for separately. Denial of these CPT codes may result in more tissue being removed unnecessarily, lower cure rates, higher recurrence rates, and potentially deaths. Quality control of the frozen section laboratory is crucial, and mandatory for CAP lab approval. This quality control involves processing of ?confirming? formalin sections off the frozen blocks of tissue is commonly performed in Mo
