[Histonet] RE: back up cryostat
Our back up died, and administration, in a thought of saving money, decided to not get it repaired as we only get about 5 frozens/week. Well, I got called in on a Saturday for a frozen, and, yup, you guessed it...the brand new one was room temperature!! (housekeeping mistakenly turned it off) I turned it on, ran and put the blade holder in the -70 freezer in blood bank, the blades in our histobath (isopentane freezer) and prayed for an hour until the frozen came down. I could only get about 2 swipes on the tissue before it started sliding off the chuck! Removed it and re-froze it until I was able to get a section!! What a day. Administration decided to repair the back up!! I vote for a back up! Lynette From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of barbara.cr...@lpnt.net [barbara.cr...@lpnt.net] Sent: Friday, September 21, 2012 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back up cryostat I would like to know what the consensus is on having a backup cryostat. Does anyone have a back up cryostat? I am nervous about not having a backup cryostat Antoinette Crill TEAM LEADER ANATOMIC PATHOLOGY X5451 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: back up cryostat
If you use anything often enough, it is wise to have a back-up, if possible. Microtomes, and especially cryostats, tend to malfunction without warning and can leave you stranded. We have a back-up that isn't ours, but we have access to it. That's another option. Make friends with someone else who has one and strike an arrangement with them. Philip Manfre, BA, HT(ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_man...@merck.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Friday, September 21, 2012 8:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back up cryostat I would like to know what the consensus is on having a backup cryostat. Does anyone have a back up cryostat? I am nervous about not having a backup cryostat Antoinette Crill TEAM LEADER ANATOMIC PATHOLOGY X5451 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: back up cryostat
We have two back ups! :) We also have back up tissue processors and microtomes as well. Sent from my iPhone On Sep 21, 2012, at 8:15 AM, Manfre, Philip philip_man...@merck.com wrote: If you use anything often enough, it is wise to have a back-up, if possible. Microtomes, and especially cryostats, tend to malfunction without warning and can leave you stranded. We have a back-up that isn't ours, but we have access to it. That's another option. Make friends with someone else who has one and strike an arrangement with them. Philip Manfre, BA, HT(ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_man...@merck.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Friday, September 21, 2012 8:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back up cryostat I would like to know what the consensus is on having a backup cryostat. Does anyone have a back up cryostat? I am nervous about not having a backup cryostat Antoinette Crill TEAM LEADER ANATOMIC PATHOLOGY X5451 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: back up cryostat
I think one of the most important things to have a back-up for is a processor. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Manfre, Philip Sent: Fri 9/21/2012 7:15 AM To: barbara.cr...@lpnt.net; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: back up cryostat If you use anything often enough, it is wise to have a back-up, if possible. Microtomes, and especially cryostats, tend to malfunction without warning and can leave you stranded. We have a back-up that isn't ours, but we have access to it. That's another option. Make friends with someone else who has one and strike an arrangement with them. Philip Manfre, BA, HT(ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_man...@merck.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet