TBS has good dyes...you can mordant them (what Bouins does) in fo
rmalin-aceto-alcohol. This is less toxic then Bouins and works
well.= nbsp; Of course if you are doing anything with breast tissue,
you don't wan= t to leave it in there but a second or two because it
is not known if it ef= fects ER/PR results.
Happy Fixing!!
PS-W= e called F-A-A juicy juice because that's what it smells like
=)
Sarah = Goebel, B.A., HT (ASCP)
Histotechnician
= div
XBiot= ech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
[DEL: (512)386-5107
:DEL]
Original Message
Subject: [Histonet] RE: embedding beads/tissue marking dyes
From: Maria Katleba [1]Maria.Ka= tl...@stjoe.org
Date: Tue, October 05, 2010 2:40 pm
To: Jacqueline Farnsworth [2]jacqueline.farnswo...@cls.ab.ca,
[3]histo...@lists.utsout= hwestern.edu [4]
Histonet@lists.utsouthwestern.edu
When you place dye on any tissue, make sure you pipette some bouins on
to t= he tissue... Apparently the bouins sets the stain...
afterwards dab the t= issue with paper towel to sop up the excess
dye/bouins.
Some dyes are better than others, but try the bouins first
Call me or email me directly, I can help you with details :)
Maria Katleba MS HT(ASCP)
Pathology Dept. Mgr
Queen of the Valley Medical Center
1000 Trancas Street
Napa CA 94558
(707) 252-4411 x3689 direct
(707) 226-4385 pager
(707) 294-9229 cell- anytime
-Original Message-
From: [5]histonet= -boun...@lists.utsouthwestern.edu
[[6]mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Jacqueline Farnsworth
Sent: Tuesday, October 05, 2010 2:14 PM
To: [7]histo...@lists.uts= outhwestern.edu
Subject: [Histonet] embedding beads/tissue marking dyes
Hi
We may be encountering some issues with our tissue marking dyes
'clogging' = up our processors. Ive heard of a system where a
coloured 'bead' is embed= ded right beside the tissue, and
subsequently cut onto the slide. This doe= s not mark the margins
obviously, but is used as a method to track like spe= cimens that are
grossed, embedded and subsequently cut in a row. (red, oran= ge,
green, blue.)
I explored the archives for embedding beads, but only found
reference to = a glass bead that is placed in the wax inside the
block, but not subsequent= ly cut.
PS: we are still troubleshooting our dyes (dilutions, brand, etc.),
but if = anyone has a brand of dye that they like and have no issues
with, I'd be th= rilled to get the information as well!
Thanks in advance,
Jacquie
Jacqueline Farnsworth
Anatomic Pathology, Tech III
Diagnostic Scientific Centre
Calgary Laboratory Services
Phone: 403-770-3588
Pager: 403-212-8223 X07630
P Please consider the environment before printing this email.
This message and any attached documents are only for the use of the
intende= d recipient(s), are confidential and may contain privileged
information. An= y unauthorized review, use, retransmission, or other
disclosure is strictly= prohibited. If you have received this message
in error, please notify the = sender immediately, and then delete the
original message. Thank you.
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References
1. 3Dmailto:maria.katl...@stjoe.org;
2. 3Dmailto:jacqueline.farnswo...@cls.a 3.
3Dmailto:Histonet@lists.utsouthwestern.edu;
4. 3Dmailto:Histonet@lists.utsouthwestern.edu;
5. 3Dmailto:histonet-boun...@lists.utsouthwestern.edu;
6. 3Dmailto:histonet-boun...@l 7.
3Dmailto:Histonet@lists.utsouthwestern.edu;
8. 3Dmailto:Histonet@lists.utsouthwestern.edu;
9. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
10. 3Dmailto:Histonet@lists.utsouthwestern.edu;
11. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
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