RE: [Histonet] Separation Artifact
If its happening to an entire batch but not to other batches then you can sorta rule out fixation unless you use different batches of fixative for different batches of samples; logical yes? To put it another way if you use the same fixative and fixation time on all batches but only one is affected then it must be an issue with the machine. Not knowing which one you are using makes this difficult but sometimes they go wrong or there's the wrong fluid dispensed. If however you are using different batches of fixatives on different batches of samples you may have one batch of fixative that's incorrectly made up. Hope that helps. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: 21 September 2009 21:27 To: Histonet Subject: [Histonet] Separation Artifact Hello histonet! I'm trying to figure out the cause of some artifact in my ffpe he skin slides. Every once and a while I get a batch of slides with this strange separation artifact mostly around melanocytes. Its like there is a space around each cell(s). Also the collagen seems to have some odd stretching. I tried recuting to make sure there was no microtomy issues but the artifact remains. I check my waterbath temp / soaked the blocks. Not soaked the blocks. I'm at a loss. This happens to entire processing batch. The doctors say they are still pathologically readable but I still want to find a way to fix this. Sent from my Verizon Wireless BlackBerry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Separation Artifact
Hello histonet! I'm trying to figure out the cause of some artifact in my ffpe he skin slides. Every once and a while I get a batch of slides with this strange separation artifact mostly around melanocytes. Its like there is a space around each cell(s). Also the collagen seems to have some odd stretching. I tried recuting to make sure there was no microtomy issues but the artifact remains. I check my waterbath temp / soaked the blocks. Not soaked the blocks. I'm at a loss. This happens to entire processing batch. The doctors say they are still pathologically readable but I still want to find a way to fix this. Sent from my Verizon Wireless BlackBerry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Separation Artifact
Dear theci...@yahoo.com (whoever this is) I would suggest that to fix the problem, make sure you fix the tissues. Shrinkage is often due to alcohol fixation rather than formal fixation Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Tuesday, 22 September 2009 6:27 AM To: Histonet Subject: [Histonet] Separation Artifact Hello histonet! I'm trying to figure out the cause of some artifact in my ffpe he skin slides. Every once and a while I get a batch of slides with this strange separation artifact mostly around melanocytes. Its like there is a space around each cell(s). Also the collagen seems to have some odd stretching. I tried recuting to make sure there was no microtomy issues but the artifact remains. I check my waterbath temp / soaked the blocks. Not soaked the blocks. I'm at a loss. This happens to entire processing batch. The doctors say they are still pathologically readable but I still want to find a way to fix this. Sent from my Verizon Wireless BlackBerry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
