[Histonet] Special stains and hoods

[Cassie P. Davis via Histonet]

25+ years in Histology, half the places I worked had vents on the wall near the 
countertop where we did specials, otherwise it was on the counterclosest to the 
sink. The only times we were time doing anything under a hood was for frozen 
section staining because of the fresh tissue, mixing sulfer chemicals for 
muscle enzyme studies, diluting acids and the bouins when we microwaved it.  As 
long as there is a sink under the hood too, I wouldn't mind, though.

Cassandra Davis
Histology Technician
AP Laboratory
302-575-8095
Email:  cda...@che-east.org



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Re: [Histonet] Special stains and hoods

[Morken, Timothy via Histonet]

I guess the question is, who told you this and what is their reference?

Most special stains don't require a hood. Only those that have volatile 
chemicals or odors that may be offensive (ie, ammonium sulfide). We have some 
areas we do specials without a hood. Our main special stain areas have a 
"comfort" hood that is not a real chemical hood but more like a hood in an oven 
hood in a commercial kitchen.  They are not certified or checked in any way and 
we do not claim they are safety hoods.



Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

-Original Message-
From: Laurie Colbert via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, August 21, 2018 7:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Special stains and hoods

I would like to know how many people perform (manual) routine special stains 
under a hood.  We do basic specials - Giemsa, AB/PAS, GMS, Trichrome, Muci - 
but we do them on the counter, not under a hood.  We have been told the 
staining needs to be done under a hood, but I have never done that in my entire 
career.
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Re: [Histonet] Special stains and hoods

[Logan, Shannon via Histonet]

That is certainly an odd request…
I have never performed any special stains under a hood
any place I have ever worked.
Always done on a counter top near a sink…
Is someone in your department extremely sensitive to odors? Or have asthma?
We have a tech who experiences attacks if there are certain chemicals in the 
air however,
she has never complained about the special stains odors.


Shannon H. Logan  B.S., HTL (ASCP)
Pathology Department

Bellin Health Memorial Hospital
744 South Webster Avenue
Green Bay, WI 54305-3400
920-433-3653  X3727



From: Laurie Colbert via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, August 21, 2018 9:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Special stains and hoods


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I would like to know how many people perform (manual) routine special stains 
under a hood.  We do basic specials - Giemsa, AB/PAS, GMS, Trichrome, Muci - 
but we do them on the counter, not under a hood.  We have been told the 
staining needs to be done under a hood, but I have never done that in my entire 
career.
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[Histonet] Special stains and hoods

[Laurie Colbert via Histonet]

I would like to know how many people perform (manual) routine special stains 
under a hood.  We do basic specials - Giemsa, AB/PAS, GMS, Trichrome, Muci - 
but we do them on the counter, not under a hood.  We have been told the 
staining needs to be done under a hood, but I have never done that in my entire 
career.
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