[Histonet] Technovit 9100 new
Hi, it there anyone who has experience in Technovit 9100 new? I'm having a hard time in polymerization process using T9100 now. I just try to follow the manufacture instruction but it didn't work. Pls give me an advice or suggestion. Thanks, Kai H ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Technovit 9100 new fails to polymerize
Hello, I got the same problem, how did you solve it ? Best regards Jean-Philippe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Technovit 9100 new fails to polymerize
Hello, As I wrote you some weeks ago, we started to use Technovit 9100 New for tissue embedding. You helped me to solve my previous problems. And I hope, you will help me to solve my present problems. Sorry, I can explain my problem as follow : The main problem is that we are having some troubles with the polymerization of Technovit 9100 New. The polymerization mixture does not want to become hard. We filled trial moulds with 3 ml of polymerization mixture. Moulds were vacuumed and sealed. We tried to perform the polymerization reaction at -30 degrees C, -20 degrees C, -4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails to polymerize. After a week, the polymerization mixture becomes gelatinous but not hard. We used the manufacturer's protocol. The preparation of the polymerization mixture was precise (I accurately prepared stock solutions several times). I cannot understand where is the problem May be it is necessary to add more activator (dibenzoyl peroxide and N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions A and B in another proportion (not 9:1). Is it necessary to prepare the solution at 4 degrees too ? What should I do? Thank you in advance, ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Technovit 9100 new fails to polymerize
We had the same problems. We switched to Immunobed and that is wonderful. Sets up every single time. Give it a try. Best regards, Nanne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean-Philippe Berteau Sent: Wednesday, November 14, 2012 4:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Technovit 9100 new fails to polymerize Hello, As I wrote you some weeks ago, we started to use Technovit 9100 New for tissue embedding. You helped me to solve my previous problems. And I hope, you will help me to solve my present problems. Sorry, I can explain my problem as follow : The main problem is that we are having some troubles with the polymerization of Technovit 9100 New. The polymerization mixture does not want to become hard. We filled trial moulds with 3 ml of polymerization mixture. Moulds were vacuumed and sealed. We tried to perform the polymerization reaction at -30 degrees C, -20 degrees C, -4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails to polymerize. After a week, the polymerization mixture becomes gelatinous but not hard. We used the manufacturer's protocol. The preparation of the polymerization mixture was precise (I accurately prepared stock solutions several times). I cannot understand where is the problem May be it is necessary to add more activator (dibenzoyl peroxide and N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions A and B in another proportion (not 9:1). Is it necessary to prepare the solution at 4 degrees too ? What should I do? Thank you in advance, ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Technovit 9100 New
Hello, Has anyone used Technovit 9100 New? If so, what company did you order through - is there a distributor in the US? Also, have you found success with this in embedding undecalcified bone? Thanks for any and all advice! Megan Silas Megan Silas | megan.r.si...@gmail.com | 847-609-6336 University of Illinois at Urbana-Champaign | Bioengineering ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Technovit 9100 New
What is it you are trying to accomplish with staining? Can you tell us more about your project? I use Acrylosin SOFT Infiltration and Embedding solutions from Dorn and Hart Microedge (just up the road from you in Villa Park) for my thin 5 micron section and Acrylosin HARD for ground sections. Feel free to ask more questions as needed. Jack Senior Histologist, BioMimetic Therapeutics Chairman, Hard Tissue Committee - National Society for Histotechnology On Oct 16, 2011, at 10:47 PM, Megan Silas sil...@illinois.edu wrote: Hello, Has anyone used Technovit 9100 New? If so, what company did you order through - is there a distributor in the US? Also, have you found success with this in embedding undecalcified bone? Thanks for any and all advice! Megan Silas Megan Silas | megan.r.si...@gmail.com | 847-609-6336 University of Illinois at Urbana-Champaign | Bioengineering ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Technovit 9100 New
Anyone using Technovit 9100 for microtome and/or ground sections? Do you use the routine Plus slides for mounting microtome sections or do the sections require another type of adhesive? When staining the ground sections, do you have any problems with certain solutions causing cracking/crazing? Cindy Baranowski, HT (ASCP) Saint Joseph's Translational Research Institute Atlanta, Ga ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
