We had the same problems. We switched to Immunobed and that is wonderful.
Sets up every single time. Give it a try. Best regards, Nanne
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean-Philippe
Berteau
Sent: Wednesday, November 14, 2012 4:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Technovit 9100 new fails to polymerize
Hello,
As I wrote you some weeks ago, we started to use Technovit 9100 New
for tissue embedding. You helped me to solve my previous problems.
And I hope, you will help me to solve my present problems.
Sorry, I can explain my problem as follow :
The main problem is that we are having some troubles with the
polymerization of Technovit 9100 New. The polymerization mixture does
not want to become hard. We filled trial moulds with 3 ml of
polymerization mixture. Moulds were vacuumed and sealed. We tried to
perform the polymerization reaction at -30 degrees C, -20 degrees C,
-4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails
to polymerize. After a week, the polymerization mixture becomes
gelatinous but not hard. We used the manufacturer's protocol. The preparation
of the polymerization mixture was precise (I accurately prepared stock
solutions several times). I cannot understand where is the problem
May be it is necessary to add more activator (dibenzoyl peroxide and
N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions A
and B in another
proportion (not 9:1).
Is it necessary to prepare the solution at 4 degrees too ?
What should I do?
Thank you in advance,
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
