Re: [Histonet] Tissue Adherence Issue
Thank you to everyone who responded back to my posting. There were so many great suggestions and ideas for us to investigate. Who better to reach out to for help on issues than our peers?! - You are awesome! THANK YOU! Deanne Knutson Supervisor Anatomic Pathology dknut...@primecare.org "Let All Be Received as Christ." -Original Message- From: Knutson, Deanne via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, July 17, 2019 9:22 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue Adherence Issue CAUTION: This email is not from a CHI source. Only click links or open attachments you know are safe. Please send as an attachment all spam/phishing and unusual emails to s...@catholichealth.net. .. Fellow Histonetters - I would appreciate your feedback on an intermittent issue that has shown up in our lab. All of a sudden, we are having difficulty with tissue specimens falling off of our slides on the IHC stains and special stains sporadically. About a year ago, we switched instruments on the IHC bench from the Leica BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer. No adherence issues with all of the validation slides that were run. We have tried various types of slides recently as well, and the issue still prevails. Would any of you mind telling me your slide workflow - type of slide used, gelatin or not used in flotation bath, how long slides are cooked, etc for your IHC slides, for your special stains slides, and even for your H slides. I would welcome your suggestions and feedback. Thank you so much!!! Deanne Knutson Supervisor Anatomic Pathology [X] "Let All Be Received as Christ." This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=YFhW2PYwN3hsZhoCqLOPHsIEIPQ6qDXkZ40AlEYUG9c=HEssDIPSD1gvhLCPOWavMQclZsG-P6T9JqKPXgdEnF0=KVKhD0nPsfF4mSH2out3qI_QbpLWEWSMqJD2c9plYe4=lmddTuDwCBd1RGTisDb0ymW0bgm0ne1cRTBN0Az2XAo= This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Adherence Issue
> > 2. Tissue Adherence Issue (Knutson, Deanne) > Hello, I think your testing tissue might be over or under fixed in formaldehyde fixative? You didn't tell what kind of tissue though. We deal with bone tissue which needs extensively bake (at 37, 46 degree over night and 58 one hour) and carefully handled. Superfrost Plus slides are used. Especially gentle heat for heat induced antigen retrieval. Try to use enzyme antigen retrieval as possible. Best luck. Dorothy Hu > Message: 2 > Date: Wed, 17 Jul 2019 09:21:49 -0500 > From: "Knutson, Deanne" > To: "'histonet@lists.utsouthwestern.edu'" > > Subject: [Histonet] Tissue Adherence Issue > Message-ID: > < > 1e0e2b14c709174b8ac2be0ae7f768330159b5756...@exchange2k7.staprimecare.org> > > Content-Type: text/plain; charset="us-ascii" > > Fellow Histonetters - > > I would appreciate your feedback on an intermittent issue that has shown > up in our lab. > All of a sudden, we are having difficulty with tissue specimens falling > off of our slides on the IHC stains and special stains sporadically. > About a year ago, we switched instruments on the IHC bench from the Leica > BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer. > No adherence issues with all of the validation slides that were run. > We have tried various types of slides recently as well, and the issue > still prevails. > > Would any of you mind telling me your slide workflow - type of slide used, > gelatin or not used in flotation bath, how long slides are cooked, etc > for your IHC slides, for your special stains slides, and even for your H > slides. > I would welcome your suggestions and feedback. > > Thank you so much!!! > > Deanne Knutson > Supervisor > Anatomic Pathology > > [X] > "Let All Be Received as Christ." > > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue Adherence Issue
We find that the amount of time slides spend baking in the oven prior to deparaffinization on our Benchmark Ultras tends to make a difference. Harder tissue like brain and bone can even benefit from 2 hours at 58*C. Andrew Dilts HTL(ASCP) Histo Supervisor, Laboratory Services Phone: (417) 269-5021 andrew.di...@coxhealth.com www.coxhealth.com -Original Message- From: Knutson, Deanne Sent: Wednesday, July 17, 2019 9:22 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue Adherence Issue Fellow Histonetters - I would appreciate your feedback on an intermittent issue that has shown up in our lab. All of a sudden, we are having difficulty with tissue specimens falling off of our slides on the IHC stains and special stains sporadically. About a year ago, we switched instruments on the IHC bench from the Leica BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer. No adherence issues with all of the validation slides that were run. We have tried various types of slides recently as well, and the issue still prevails. Would any of you mind telling me your slide workflow - type of slide used, gelatin or not used in flotation bath, how long slides are cooked, etc for your IHC slides, for your special stains slides, and even for your H slides. I would welcome your suggestions and feedback. Thank you so much!!! Deanne Knutson Supervisor Anatomic Pathology [X] "Let All Be Received as Christ." This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. CoxHealth – ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intendedrecipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Adherence Issue
Fellow Histonetters - I would appreciate your feedback on an intermittent issue that has shown up in our lab. All of a sudden, we are having difficulty with tissue specimens falling off of our slides on the IHC stains and special stains sporadically. About a year ago, we switched instruments on the IHC bench from the Leica BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer. No adherence issues with all of the validation slides that were run. We have tried various types of slides recently as well, and the issue still prevails. Would any of you mind telling me your slide workflow - type of slide used, gelatin or not used in flotation bath, how long slides are cooked, etc for your IHC slides, for your special stains slides, and even for your H slides. I would welcome your suggestions and feedback. Thank you so much!!! Deanne Knutson Supervisor Anatomic Pathology [X] "Let All Be Received as Christ." This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
