Re: [Histonet] Toluidine Blue and staining archaeological residues.
Hello Paula, Thanks for the reply. We do use IKI regualarly for starch staining, however once the slide is stained it is difficult to counterstain to detect animal residues which might be present. The residues from grindstones have lost a lot of their microsopic diagnostic properties and we are hponing that staining the residues will help differentiate between intensive specialised seed grinders versus multi-purpose expedient grinders. I am finding that the Phloroglucinol stain is light sensitive and therefore not permanent which allows for some counterstaining but still the technique is a work in progress. Any more thoughts most welcome. Regards Birgitta Stephenson Research Microscopy Lab, University of Queensland On Tue, 20 Jul 2010 06:51:09 -0700 (PDT), Paula Pierce cont...@excaliburpathology.com said: Starch (plants) + Iodine = Black solution. From: Birgitta Stephenson bstep...@fastmail.fm To: histonet@lists.utsouthwestern.edu Sent: Mon, July 19, 2010 8:19:27 PM Subject: [Histonet] Toluidine Blue and staining archaeological residues. Hello Histonetters, I am working with archaeological residues lifted from Holocene grindstones. I was trying to find a stain that could differentiate between plant and animal tissue in one hit. I have been using buffered Toluidine Blue solutions however given that these are ancient residues which are lifted using 20ul of water, the subtle colour differences between blues, purples, and violets have not been useful. I was looking at the possibility of counterstaining the Toluidine Blue stained residues with say Phloroglucinol or IKI which would highlight the plant component of the residue and then see if what was left looked like collagen. Has anyone tried this type of counterstaining? OR does anyone know of a stain that would colour differently for plant and animals understanding that this is not a tissue section but microscopic residues in solution? Thanks Birgitta Stephenson The Research Microscopy Laboratory, University of Queensland. -- Birgitta Stephenson bstep...@fastmail.fm -- http://www.fastmail.fm - Access your email from home and the web ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Birgitta Stephenson bstep...@fastmail.fm -- http://www.fastmail.fm - Access your email from home and the web ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Toluidine Blue and staining archaeological residues.
Starch (plants) + Iodine = Black solution. From: Birgitta Stephenson bstep...@fastmail.fm To: histonet@lists.utsouthwestern.edu Sent: Mon, July 19, 2010 8:19:27 PM Subject: [Histonet] Toluidine Blue and staining archaeological residues. Hello Histonetters, I am working with archaeological residues lifted from Holocene grindstones. I was trying to find a stain that could differentiate between plant and animal tissue in one hit. I have been using buffered Toluidine Blue solutions however given that these are ancient residues which are lifted using 20ul of water, the subtle colour differences between blues, purples, and violets have not been useful. I was looking at the possibility of counterstaining the Toluidine Blue stained residues with say Phloroglucinol or IKI which would highlight the plant component of the residue and then see if what was left looked like collagen. Has anyone tried this type of counterstaining? OR does anyone know of a stain that would colour differently for plant and animals understanding that this is not a tissue section but microscopic residues in solution? Thanks Birgitta Stephenson The Research Microscopy Laboratory, University of Queensland. -- Birgitta Stephenson bstep...@fastmail.fm -- http://www.fastmail.fm - Access your email from home and the web ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Toluidine Blue and staining archaeological residues.
Hello Histonetters, I am working with archaeological residues lifted from Holocene grindstones. I was trying to find a stain that could differentiate between plant and animal tissue in one hit. I have been using buffered Toluidine Blue solutions however given that these are ancient residues which are lifted using 20ul of water, the subtle colour differences between blues, purples, and violets have not been useful. I was looking at the possibility of counterstaining the Toluidine Blue stained residues with say Phloroglucinol or IKI which would highlight the plant component of the residue and then see if what was left looked like collagen. Has anyone tried this type of counterstaining? OR does anyone know of a stain that would colour differently for plant and animals understanding that this is not a tissue section but microscopic residues in solution? Thanks Birgitta Stephenson The Research Microscopy Laboratory, University of Queensland. -- Birgitta Stephenson bstep...@fastmail.fm -- http://www.fastmail.fm - Access your email from home and the web ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
