Re: [Histonet] Unstained slides - how long are they good for?
Hi Everyone! I have seen unstained slides save a patient from re-biopsy many times. Usually it will be a case where a patient has a known diagnosis, like lung cancer. In these types of cases after diagnosis molecular testing (and IHC for PD-L1) is usually ordered. There have been countless times that I can recall where a few unstained slides on a biopsy with scant tumor was able to get us results for PD-L1, ALK FISH, and ROS1 FISH. Often in these types of a cases a touch prep can be used for Next Generation Sequencing or PCR testing like EGFR or BRAF, allowing for the full panel of molecular tests to be performed. For cases that are small specimens I would prefer to have unstained slides to fall back on for patient convenience, client satisfaction, and quicker TAT of molecular testing. Re-biopsy and re-diagnosing the new sample costs money to the patient and payers and having some unstained slides can often save those costs providing more value to the original biopsy. Sometimes when we try to save money in the lab it can result in more money being spent on healthcare overall. It is true that some antigens become more difficult to stain over time and storage is an important consideration. Limiting the production of unstained slides to small and scant needle may make storage more practical. Just some more things to consider. Sincerely, Mark Tarango On Thu, Aug 16, 2018 at 4:48 PM, P Sicurello via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello My Fellow Histologists, > > Happy Friday Eve. > > The question has come up.. How long are *unstained* slides good for? > Not for H but tests like IHC and molecular testing. These slides have > been cut, stored at room temperature, not sealed in anyway, and kept in a > cardboard box. > > Please let me know what your opinions are and what your retention policy is > concerning *unstained* slides. > > Thanks oodles. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides - how long are they good for?
To expand on what we do at our research lab; we use 6 months as a standard maximum age of unstained slides. Also in the staining protocol for each antibodyl we have a specific shelf life for the diluted Antibody and a maximum age of unstained slides. Jamie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides - how long are they good for?
I agree with Jamie Only a few Ags are "oxidised" ( that's the term used, I recall) but, don't let it be YOUR protein of interest. If you really are concerned, cut fresh sections and immunostain along with your stored sections. Imho: cut as few sections as you need. Store any unused at 4C Best wishes Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides - how long are they good for?
I’m a histology workflow consultant that visits many AP laboratories each year. Almost every laboratory has a different retention policy. The average of most laboratories is to hold onto unstained slides for three weeks after final sign out. Typically the unstained slide can be held for a long period of time if used just for morphological staining. However if the unstained slides is going to be used for IHC or molecular testing, the antigenicity of the slide begins degrading at the point of cutting. Typically, however, for a high-quality IHC staining, if stored at room temperature, the unstained slide should not be held much longer than one month. And even at that time frame you will begin to see the degrading of the stain quality. If the slides are kept in a closed box, in refrigerator, they have longer retention. Typically up to 2 to 3 months. I hope this helps Sent from my iPhone > On Aug 16, 2018, at 19:48, P Sicurello wrote: > > Hello My Fellow Histologists, > > Happy Friday Eve. > > The question has come up.. How long are *unstained* slides good for? > Not for H but tests like IHC and molecular testing. These slides have > been cut, stored at room temperature, not sealed in anyway, and kept in a > cardboard box. > > Please let me know what your opinions are and what your retention policy is > concerning *unstained* slides. > > Thanks oodles. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides - how long are they good for?
Paula, since it is variable we strive to not have unstained slides. We had kept them indefinitely, then when storage was overwhelming us we reduced it to 2 months maximum. Now we require request for unstained to be ordered in the system and delivered to the pathologist. We do not hold any in the lab. We recut when new stains are ordered. In the past we had routinely cut extras "just in case" but ended up with thousands of unstained slides that were never used. Instead we trained everyone to reduce wastage and get good sections from a cut block with minimal facing. We have not stored unstained sections for many years and they do not seem to be missed. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, August 16, 2018 4:49 PM To: HistoNet Subject: [Histonet] Unstained slides - how long are they good for? Hello My Fellow Histologists, Happy Friday Eve. The question has come up.. How long are *unstained* slides good for? Not for H but tests like IHC and molecular testing. These slides have been cut, stored at room temperature, not sealed in anyway, and kept in a cardboard box. Please let me know what your opinions are and what your retention policy is concerning *unstained* slides. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides - how long are they good for?
Will definitely depend on the antibody you are using. Some references: Jacobs, T. W., Prioleau, J. E., Stillman, I. E., & Schnitt, S. J. (1996). Loss of tumor marker-immunostaining intensity on stored paraffin slides of breast cancer. JNCI: Journal of the National Cancer Institute, 88(15), 1054-1059. Manne, U., MYERS, R. B., SRIVASTAVA, S., & GRIZZLE, W. E. (1997). Re: loss of tumor marker-immunostaining intensity on stored paraffin slides of breast cancer. Journal of the National Cancer Institute, 89(8), 585-586. Bertheau, P., Cazals-Hatem, D., Meignin, V., de Roquancourt, A., Vérola, O., Lesourd, A., ... & Janin, A. (1998). Variability of immunohistochemical reactivity on stored paraffin slides. Journal of clinical pathology, 51(5), 370-374. Olapade-Olaopa, E. O., Mackay, E. H., & Habib, F. K. (1998). Variability of immunohistochemical reactivity on stored paraffin slides. Journal of clinical pathology, 51(12), 943. Wester, K., Wahlund, E., Sundström, C., Ranefall, P., Bengtsson, E., Russell, P. J., ... & Busch, C. (2000). Paraffin section storage and immunohistochemistry: effects of time, temperature, fixation, and retrieval protocol with emphasis on p53 protein and MIB1 antigen. Applied Immunohistochemistry & Molecular Morphology, 8(1), 61-70. van den Broek, L. J., & van de Vijver, M. J. (2000). Assessment of problems in diagnostic and research immunohistochemistry associated with epitope instability in stored paraffin sections. Applied Immunohistochemistry & Molecular Morphology, 8(4), 316-321. Olapade-Olaopa, E. O., Ogunbiyi, J. O., MacKay, E. H., Muronda, C. A., Alonge, T. O., Danso, A. P., ... & Wong, A. J. (2001). Further characterization of storage-related alterations in immunoreactivity of archival tissue sections and its implications for collaborative multicenter immunohistochemical studies. Applied Immunohistochemistry & Molecular Morphology, 9(3), 261-266. Mirlacher, M., Kasper, M., Storz, M., Knecht, Y., Dürmüller, U., Simon, R., ... & Sauter, G. (2004). Influence of slide aging on results of translational research studies using immunohistochemistry. Modern pathology, 17(11), 1414. DiVito, K. A., Charette, L. A., Rimm, D. L., & Camp, R. L. (2004). Long-term preservation of antigenicity on tissue microarrays. Laboratory investigation, 84(8), 1071. Fergenbaum, J. H., Garcia-Closas, M., Hewitt, S. M., Lissowska, J., Sakoda, L. C., & Sherman, M. E. (2004). Loss of antigenicity in stored sections of breast cancer tissue microarrays. Cancer Epidemiology and Prevention Biomarkers, 13(4), 667-672. Hameed, O., & Humphrey, P. A. (2009). Immunohistochemical evaluation of prostate needle biopsies using saved interval sections vs new recut sections from the block: a prospective comparison. American journal of clinical pathology, 131(5), 683-688. Xie, R., Chung, J. Y., Ylaya, K., Williams, R. L., Guerrero, N., Nakatsuka, N., ... & Hewitt, S. M. (2011). Factors influencing the degradation of archival formalin-fixed paraffin-embedded tissue sections. Journal of Histochemistry & Cytochemistry, 59(4), 356-365. Seidu, M. A., Adams, A. R., Gyasi, R. K., Tettey, Y., Nkansah, D. O., & Wiredu, E. K. (2013). Immunoreactivity of some epitopes in longtime inappropriately stored paraffin-embedded tissues. Journal of Histotechnology, 36(2), 59-64. Nuovo, A. J., Garofalo, M., Mikhail, A., Nicol, A. F., Vianna-Andrade, C., & Nuovo, G. J. (2013). The effect of aging of formalin-fixed paraffin-embedded tissues on the in situ hybridization and immunohistochemistry signals in cervical lesions. Diagnostic Molecular Pathology, 22(3), 164-173. Grillo, F., Bruzzone, M., Pigozzi, S., Prosapio, S., Migliora, P., Fiocca, R., & Mastracci, L. (2017). Immunohistochemistry on old archival paraffin blocks: is there an expiry date?. Journal of Clinical Pathology, jclinpath-2017. Giunchi, F., Degiovanni, A., Daddi, N., Trisolini, R., Dell'Amore, A., Agostinelli, C., ... & Fiorentino, M. (2018). Fading With Time of PD-L1 Immunoreactivity in Non-Small Cells Lung Cancer Tissues: A Methodological Study. Applied Immunohistochemistry & Molecular Morphology, 26(7), 489-494. -Original Message- From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, 17 August 2018 9:49 AM To: HistoNet Subject: [Histonet] Unstained slides - how long are they good for? Hello My Fellow Histologists, Happy Friday Eve. The question has come up.. How long are *unstained* slides good for? Not for H but tests like IHC and molecular testing. These slides have been cut, stored at room temperature, not sealed in anyway, and kept in a cardboard box. Please let me know what your opinions are and what your retention policy is concerning *unstained* slides. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive
Re: [Histonet] Unstained slides - how long are they good for?
It depends on the stability of the protein and fixation, some are stable for a week some for years. We use 6 months as a standard. Jamie On August 16, 2018 4:59:34 PM P Sicurello via Histonet wrote: > Hello My Fellow Histologists, > > Happy Friday Eve. > > The question has come up.. How long are *unstained* slides good for? > Not for H but tests like IHC and molecular testing. These slides have > been cut, stored at room temperature, not sealed in anyway, and kept in a > cardboard box. > > Please let me know what your opinions are and what your retention policy is > concerning *unstained* slides. > > Thanks oodles. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Unstained slides - how long are they good for?
Hello My Fellow Histologists, Happy Friday Eve. The question has come up.. How long are *unstained* slides good for? Not for H but tests like IHC and molecular testing. These slides have been cut, stored at room temperature, not sealed in anyway, and kept in a cardboard box. Please let me know what your opinions are and what your retention policy is concerning *unstained* slides. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
