Hi Matt,
I have worked a bit with this detection kit and my impression is that is
extremely sensitive and should be able to boost signal considerably. I have
also been able to shave off an average of 30 minutes off several stains.
(Cyclin D1, which is one of our longer stains at 4 hours is down to 2.5)
You can further enhance the stain (or shorten the time) with the amp kit, but I
did not find it necessary. I thought it was particularly good on nuclear
stains. There is also a good deal of flexibility with the kit and you should
be able to get a great stain in less time.
Hope that was helpful.
Ashley Troutman BS, HT(ASCP) QIHC
Immunohistochemistry Supervisor
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4531
Nashville, TN 37232
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Hello All,
Is anyone using Ventana's OptiView detection kit? If so please let me know
what you impression is.
Thank you,
Matt Brooks, BS, HT (ASCP)
Histology Supervisor
InCyte Pathology
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