Re: [Histonet] Victoria blue for lung tissue
Whoops, The Ventana does not use Victoria blue, my mistake. They have a resorcin fuchsin solution (known as Hart's method) not Miller Victoria blue. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: Tony Henwood (SCHN) Sent: Saturday, 27 July 2019 10:44 AM To: Bob Richmond Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Victoria blue for lung tissue Victoria Blue is the dye used in Miller's Stain for Elastic Tissue. It is also used in the Roche Ventana Benchmark Stainer to stain elastic Tissue: Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148-149 Karen Percival & Zaher Radi (2017) Comparison of five elastin histochemical stains to identify pulmonary small vasculature, Journal of Histotechnology, 40:3, 73-78 Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in Human Skin" Histologic 32(1): 12 Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol 1996: 23: 37-42. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Bob Richmond via Histonet Sent: Saturday, July 27, 2019 4:10 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Victoria blue for lung tissue Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE lung tissue to exam thickness of artery. Could anybody recommend a good vendor of this reagent kit?<< You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and several others. I couldn't find anyone who offers a kit, only the dry dye. There are other dyes called Victoria Blue, reported to give the same results. You'd have to find a method for preparing it as an elastic stain, and I couldn't find such a method either with Google or in my old books. The requester needs to supply you with a method. I suspect the requester is reading an old article. There are stains for elastic tissue (which is I suppose what you want to "exam thickness of artery") that are a lot easier to get. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Victoria blue for lung tissue
There is also a Kit and the ready to use solution available from MORPHISTO Germany. Just contact the sales office: i...@morphisto.de Cheers Michael > Am 27.07.2019 um 23:53 schrieb Tony Henwood (SCHN) via Histonet > : > > Methods found here: > > https://www.ihcworld.com/_protocols/special_stains/miller's_elastic_ellis.htm > > http://www.histosearch.com/histonet/Dec98/Millersstainforelasticfib.html > > https://www.sakuraus.com/getattachment/774d89be-4b32-4d69-bb02-8dea75c52c0c/858 > > ? > > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children's Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > From: Bob Richmond > Sent: Saturday, July 27, 2019 11:27:54 AM > To: Tony Henwood (SCHN) > Subject: Re: [Histonet] Victoria blue for lung tissue > > Is this method published anywhere that "Amy" would likely have access to? > This looked to me like one of those cases of the blind leading the blind that > are all to common in research involving histochemistry. > > On Fri, Jul 26, 2019 at 8:44 PM Tony Henwood (SCHN) > mailto:tony.henw...@health.nsw.gov.au>> wrote: > Victoria Blue is the dye used in Miller's Stain for Elastic Tissue. > It is also used in the Roche Ventana Benchmark Stainer to stain elastic > Tissue: > > Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148-149 > > Karen Percival & Zaher Radi (2017) Comparison of five elastin histochemical > stains to identify pulmonary small vasculature, Journal of Histotechnology, > 40:3, 73-78 > > Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in Human > Skin" Histologic 32(1): 12 > > Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol > 1996: 23: 37-42. > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children's Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > ____ > From: Bob Richmond via Histonet > mailto:histonet@lists.utsouthwestern.edu>> > Sent: Saturday, July 27, 2019 4:10 AM > To: > Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Victoria blue for lung tissue > > Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE > lung tissue to exam thickness of artery. Could anybody recommend a good > vendor of this reagent kit?<< > > You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and > several others. I couldn't find anyone who offers a kit, only the dry dye. > There are other dyes called Victoria Blue, reported to give the same > results. > > You'd have to find a method for preparing it as an elastic stain, and I > couldn't find such a method either with Google or in my old books. The > requester needs to supply you with a method. I suspect the requester is > reading an old article. There are stains for elastic tissue (which is I > suppose what you want to "exam thickness of artery") that are a lot easier > to get. > > Bob Richmond > Samurai Pathologist > Maryville TN > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential > information. If you are not the intended recipient, please delete it and > notify the sender. > > Views expressed in this message are those of the individual sender, and are > not necessarily the views of NSW Health or any of its entities. > > > This message is intended for the addressee named and may contain confidential > information. If you are not the intended recipient, please delete it and > notify the sender. > > Views expressed in this message are those of the individual sender, and are > not necessarily the views of NSW Health or any of its entities. > ___ > Histonet mailing list > Histonet@li
Re: [Histonet] Victoria blue for lung tissue
Miller's stain is available from VWR. I do not recommend the BSS to stain elastin - it is not strong enough and misses the fine fibres. Kind regards, Penny :) Penny Marr Senior BMS C/- Histology Conquest Hospital St Leonards-on-Sea TN37 7RD penny.m...@nhs.net (01424) 758023 -Original Message- From: Tony Henwood (SCHN) [mailto:tony.henw...@health.nsw.gov.au] Sent: 27 July 2019 01:44 To: Bob Richmond Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Victoria blue for lung tissue Victoria Blue is the dye used in Miller's Stain for Elastic Tissue. It is also used in the Roche Ventana Benchmark Stainer to stain elastic Tissue: Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148-149 Karen Percival & Zaher Radi (2017) Comparison of five elastin histochemical stains to identify pulmonary small vasculature, Journal of Histotechnology, 40:3, 73-78 Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in Human Skin" Histologic 32(1): 12 Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol 1996: 23: 37-42. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Bob Richmond via Histonet Sent: Saturday, July 27, 2019 4:10 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Victoria blue for lung tissue Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE lung tissue to exam thickness of artery. Could anybody recommend a good vendor of this reagent kit?<< You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and several others. I couldn't find anyone who offers a kit, only the dry dye. There are other dyes called Victoria Blue, reported to give the same results. You'd have to find a method for preparing it as an elastic stain, and I couldn't find such a method either with Google or in my old books. The requester needs to supply you with a method. I suspect the requester is reading an old article. There are stains for elastic tissue (which is I suppose what you want to "exam thickness of artery") that are a lot easier to get. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in relation to its contents. To do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland. NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and other accredited email services. For more information and to find out how you can switch, https://portal.nhs.net/help/joiningnhsmail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Victoria blue for lung tissue
Methods found here: https://www.ihcworld.com/_protocols/special_stains/miller's_elastic_ellis.htm http://www.histosearch.com/histonet/Dec98/Millersstainforelasticfib.html https://www.sakuraus.com/getattachment/774d89be-4b32-4d69-bb02-8dea75c52c0c/858 ? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Bob Richmond Sent: Saturday, July 27, 2019 11:27:54 AM To: Tony Henwood (SCHN) Subject: Re: [Histonet] Victoria blue for lung tissue Is this method published anywhere that "Amy" would likely have access to? This looked to me like one of those cases of the blind leading the blind that are all to common in research involving histochemistry. On Fri, Jul 26, 2019 at 8:44 PM Tony Henwood (SCHN) mailto:tony.henw...@health.nsw.gov.au>> wrote: Victoria Blue is the dye used in Miller's Stain for Elastic Tissue. It is also used in the Roche Ventana Benchmark Stainer to stain elastic Tissue: Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148-149 Karen Percival & Zaher Radi (2017) Comparison of five elastin histochemical stains to identify pulmonary small vasculature, Journal of Histotechnology, 40:3, 73-78 Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in Human Skin" Histologic 32(1): 12 Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol 1996: 23: 37-42. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Bob Richmond via Histonet mailto:histonet@lists.utsouthwestern.edu>> Sent: Saturday, July 27, 2019 4:10 AM To: Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Victoria blue for lung tissue Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE lung tissue to exam thickness of artery. Could anybody recommend a good vendor of this reagent kit?<< You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and several others. I couldn't find anyone who offers a kit, only the dry dye. There are other dyes called Victoria Blue, reported to give the same results. You'd have to find a method for preparing it as an elastic stain, and I couldn't find such a method either with Google or in my old books. The requester needs to supply you with a method. I suspect the requester is reading an old article. There are stains for elastic tissue (which is I suppose what you want to "exam thickness of artery") that are a lot easier to get. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Victoria blue for lung tissue
Victoria Blue is the dye used in Miller's Stain for Elastic Tissue. It is also used in the Roche Ventana Benchmark Stainer to stain elastic Tissue: Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148–149 Karen Percival & Zaher Radi (2017) Comparison of five elastin histochemical stains to identify pulmonary small vasculature, Journal of Histotechnology, 40:3, 73-78 Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in Human Skin" Histologic 32(1): 12 Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol 1996: 23: 37-42. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Bob Richmond via Histonet Sent: Saturday, July 27, 2019 4:10 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Victoria blue for lung tissue Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE lung tissue to exam thickness of artery. Could anybody recommend a good vendor of this reagent kit?<< You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and several others. I couldn't find anyone who offers a kit, only the dry dye. There are other dyes called Victoria Blue, reported to give the same results. You'd have to find a method for preparing it as an elastic stain, and I couldn't find such a method either with Google or in my old books. The requester needs to supply you with a method. I suspect the requester is reading an old article. There are stains for elastic tissue (which is I suppose what you want to "exam thickness of artery") that are a lot easier to get. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Victoria blue for lung tissue
Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE lung tissue to exam thickness of artery. Could anybody recommend a good vendor of this reagent kit?<< You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and several others. I couldn't find anyone who offers a kit, only the dry dye. There are other dyes called Victoria Blue, reported to give the same results. You'd have to find a method for preparing it as an elastic stain, and I couldn't find such a method either with Google or in my old books. The requester needs to supply you with a method. I suspect the requester is reading an old article. There are stains for elastic tissue (which is I suppose what you want to "exam thickness of artery") that are a lot easier to get. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Victoria Blue for lung tissue
Hello Histonet, I was asked to do Victoria Blue stain on rodent FFPE lung tissue to exam thickness of artery. Could anybody recommend a good vendor of this reagent kit? Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
