[Histonet] cryojane question
Happy Friday! We recently purchased a cryojane for sectioning frozen human and rat cartilage. I am having some issues with my cartilage sections. I am using the 4X slides with 2 flashes. The sections look beautiful, until I put them into buffer to do staining. I am getting folds and the sections are lifting off during IHC staining. I am staining either by hand (on flat trays, old-fashioned style) or in the sequenza racks. Luckily, the cartilage is not not coming off the slides completely, so I have been able to get the data I need. I was wondering if anyone had any tips for me to prevent the folds or the lift-off. Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cryojane question
Histonetters, I just looked up what a cryojane was, and it's pretty neat! Does anyone else use this? The one flaw seems to be that you can only put one section on a slide (or at least that the way it's depicted here: http://www.instrumedics.com/cryojanetapetransferprocess.htm ) which makes it pretty time consuming. Also does the uv step interfere with in situ protocols? I guess not since the DNA/RNA is already transcribed and fixed and therefore wouldn't be mutated. Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryojane question
Emily You can put more than one section on a slide if you need to, but in our experience it does not work as nicely as depicted all of the time, it can be a bit tricky to work with on undecalcifed bone and harder tissues. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, June 18, 2010 8:10 AM To: Kim Merriam; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryojane question Histonetters, I just looked up what a cryojane was, and it's pretty neat! Does anyone else use this? The one flaw seems to be that you can only put one section on a slide (or at least that the way it's depicted here: http://www.instrumedics.com/cryojanetapetransferprocess.htm ) which makes it pretty time consuming. Also does the uv step interfere with in situ protocols? I guess not since the DNA/RNA is already transcribed and fixed and therefore wouldn't be mutated. Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryojane question
Does this kind of seem unnecessary? What are the applicatio= ns that
would make this Cryo-tape beneficial. I have always used the
paintbrush method which takes 3 seconds, and has always worked
perfect.nbs= p; This looks like it would take several minutes? It
looks cool and a= ll, but just confused as to what application you
need this for; it's defini= tely not cheaper than the brush?
Sarah Goebel, B.A., HT (ASCP)= /div
Histotechnician
XBiotech USA Inc.
= em
8201 East = Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: Re: [Histonet] cryojane question
From: Emily Sours [1]talulahgos= h...@gmail.com
Date: Fri, June 18, 2010 7:10 am
To: Kim Merriam [2]kmerriam200= 3...@yahoo.com,
[3]histo...@lists.utsou= thwestern.edu
Histonetters,
I just looked up what a cryojane was, and it's pretty neat!
Does anyone else use this?
The one flaw seems to be that you can only put one section on a slide
(or a= t
least that the way it's depicted here:
[4]htt= p://www.instrumedics.com/cryojanetapetransferprocess.htm )
which makes it pretty time consuming. Also does the uv step interfere
with in situ protocols? I guess not since the DNA/RNA is already
transcribed and fixed and therefore wouldn't be mutated.
Emily
Towns are like people. Old ones often have character, the new ones are
interchangeable.
--Wallace Stegner, Angle of Repose
___
Histonet mailing list
[5]histo...@lists.utsou= thwestern.edu
[6]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet
References
1. 3Dmailto://talulahg...@gmail.com/
2. 3Dmailto://kmerriam2...@yahoo.com/
3. 3Dmailto://histonet@lists.utsouthwestern.edu/
4. 3Dhttp://www.instrumedics.com/cryojanetapetransferprocess.htm;
5. 3Dmailto://Histonet@lists.utsouthwestern.edu/
6. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
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RE: [Histonet] cryojane question
I've used it for serial frozen sections in the past; worked very well for that application. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, June 18, 2010 9:10 AM To: Kim Merriam; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryojane question Histonetters, I just looked up what a cryojane was, and it's pretty neat! Does anyone else use this? The one flaw seems to be that you can only put one section on a slide (or at least that the way it's depicted here: http://www.instrumedics.com/cryojanetapetransferprocess.htm ) which makes it pretty time consuming. Also does the uv step interfere with in situ protocols? I guess not since the DNA/RNA is already transcribed and fixed and therefore wouldn't be mutated. Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
