Re: [Histonet] destain for IHC
Hi If you are using the peroxidase method the hydrogen peroxide step will remove the eosin and some of the haematoxylin. No need to do a decolorisation step before staining. As Gudren said the only problem may be not having coated slides. Good luck. Sadly retired histologist Tony Sent from my iPhone > On 10 Feb 2023, at 6:07 am, Katleba, Maria via Histonet > wrote: > > Oh no!!! We cut all slides on + > > BUT you can do this. It’s what we do when we have H on plain slides. > > Whether you have a dry unstain from another lab that sent u a slide, or your > H that you just took the coverslip off… > > Place it in. Coplin jar with 1-3 DROPS of formalin, cover, place In 60ft > degree oven for 60 min. You want the bottom of the jar to be moist not > floating in it > > After 60 min remove and open UNDER A HOOD (it’s a vaporized carcinogen) > Air out 10-15 min… place on stainer > > The drops vaporize and the aldehyde goes through tissue and make it adhere to > the slide permanently. > > I do this when I cut nails or anything that won’t stay on a plus slide. > > Maria > > Sent from my iPhone > >> On Feb 8, 2023, at 11:21 PM, Gudrun Lang via Histonet >> wrote: >> > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] destain for IHC
Oh no!!! We cut all slides on + BUT you can do this. It’s what we do when we have H on plain slides. Whether you have a dry unstain from another lab that sent u a slide, or your H that you just took the coverslip off… Place it in. Coplin jar with 1-3 DROPS of formalin, cover, place In 60ft degree oven for 60 min. You want the bottom of the jar to be moist not floating in it After 60 min remove and open UNDER A HOOD (it’s a vaporized carcinogen) Air out 10-15 min… place on stainer The drops vaporize and the aldehyde goes through tissue and make it adhere to the slide permanently. I do this when I cut nails or anything that won’t stay on a plus slide. Maria Sent from my iPhone > On Feb 8, 2023, at 11:21 PM, Gudrun Lang via Histonet > wrote: > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] destain for IHC
Hi Nancy, You should have no problem doing so. If you want proof, just run a couple of your IHC control sections through the H stainer as you would for a patient specimen (so right through to coverslipping) and then start the process of removing coverslip, hydration, destain in acid alcohol and then run slides on your IHC platform as you would -without the dewax step obviously. :-) *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *If God made a drink better than whiskey he kept it for himself! * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] destain for IHC
We do restaining for IHC also, but without destaining at all. Hematoxylin doesn't matter because nuclei are stained after IHC with hematoxylin anyway. And Eosin gets lost through all the washing in buffer. The problem is, that HE section are usually mounted on non-adhesive slides, and they float away during the procedure. Gudrun -Ursprüngliche Nachricht- Von: Nancy Schmitt via Histonet [mailto:histonet@lists.utsouthwestern.edu] Gesendet: Mittwoch, 8. Februar 2023 18:29 An: Histonet Betreff: [Histonet] destain for IHC Hello- Has anyone had success destaining an H and then running IHC on the same slide? Thanks! Nancy Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] destain for IHC
Nancy, I would say that it can be done in a very particular situation: i.e.: no more tissue available, and type of IHC stain. If it is a dual stain, pathologist can not obtain the good results. In the lab I worked before, it was done, but this technique was not part of the protocol. Hope it helps, René Sent from my iPhone > On Feb 8, 2023, at 10:35, Bacon, Charles via Histonet > wrote: > > Hi Nancy, > > We use our Acid alcohol solution from our AFB Stain. It pulls off the Eosin > and leaves a faded Hematoxylin behind. We counterstain in Hematoxylin anyway > so we find this protocol effective. Then we remove the deparaffinization step > from our IHC protocol and run the slide. > > 1% Acid Alcohol > 70% Alcohol..99ml > HCL...1ml > > Chuck Bacon, HTL(ASCP)CM > Supervisor Histology > Baystate Medical Center > 361 Whitney Ave., Holyoke, MA 01040 > Telephone: 413-322-4786 Fax: 413-322-4790 > charles.ba...@baystatehealth.org > > -Original Message- > From: Cooper, Brian > Sent: Wednesday, February 8, 2023 12:46 PM > To: Nancy Schmitt ; Histonet > > Subject: Re: [Histonet] destain for IHC > > We used to do this all the time in my old reference lab. > > > Thanks, > > > > Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor > > Department of Pathology and Laboratory Medicine > > Children's Hospital Los Angeles > > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > > Ph: 323.361.3357 > > bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu> > > ____________ > From: Nancy Schmitt via Histonet > Sent: Wednesday, February 8, 2023 9:29:13 AM > To: Histonet > Subject: [Histonet] destain for IHC (EXTERNAL EMAIL) > > CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside > CHLA. Do not open attachments, click on links, or respond unless you expected > this message and recognize the email address: > histonet-boun...@lists.utsouthwestern.edu. > > Hello- > > Has anyone had success destaining an H and then running IHC on the same > slide? > > Thanks! > > Nancy > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health and > is intended for the sole use of the intended recipient(s). It may contain > information that is privileged and confidential. Any unauthorized review, > use, disclosure, or distribution is prohibited. If you are not the intended > recipient, please delete this message, and reply to the sender regarding the > error in a separate email. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.com/v3/__http://secure-web.cisco.com/18_6RdTZTUgn6UmJHW1nn3zKxUTmGLFAErCLSQhQ4ue4GO5-KLRwTACOn7-7a4Er6_TzsYZZ4UJExAdC7pqHx-oxEawdx8S5CkePiIsj96I7voATRTAboDo7E2qiK9afrwIVXNNuBtZ8SbVV19aaqLNv_C6W09X2oFytd1wMK-vTSuvU_07Lyhi7dgDV_cJFs_J64GiPgdhlO7IfatGhbbblWSNlWIzmpleM9CYPNaXdR-M97emBmUM5kzsc1I7oLqfd8_UkGkyx7mm3fglIjsE-lgwHGa4s8mnu-E2FVrzwZy--we--SKanQ_Ey8mdl4n7ztSRvyeZBx4VChcrr6mD8SvTxjQrDW6C4Az7JEUIYVsLyZRIiBGlYk5KptoQ5LaF68M_Cgw5-SK01KtqhBCoxSljHTzsEdEkA4-SxEHMJWcIpEMD1w6cBoLG0RGerMMY9vRHwAIHAv-xNMXT30yQ/http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet__;JSUlJSUl!!P_vj-BUkwjF5!f6gMMu3epLW9iWatLaXyHemacm0hDOoYOXCaMU5Ijo60vj-bxgHDrpUHZFZGfrf594v9R-jNYLfSmKM-MmAw_V6es7aYOBgKp9eltUXF9cY0L4I$ > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential or > legally privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original > message. > > > -- > Please view our annual report at http://www.bhannualreport.org > > > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may > contain confidential and privileged information for the use of the designated > recipients named above. If you are not the intended recipient, you are hereby > notified that you have received this communication in error and that any > review, disclosure, dissemination, distribution or copying of it or its > contents is prohibited. If you have received this communication in error, > please reply to the sender immediately or by telephone at 413-794- and > destroy all copies of this communication and any attachments. For further > information regarding Baystate Health's privacy policy, please visit our > Internet site at
Re: [Histonet] destain for IHC
Hi Nancy, We use our Acid alcohol solution from our AFB Stain. It pulls off the Eosin and leaves a faded Hematoxylin behind. We counterstain in Hematoxylin anyway so we find this protocol effective. Then we remove the deparaffinization step from our IHC protocol and run the slide. 1% Acid Alcohol 70% Alcohol..99ml HCL...1ml Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786 Fax: 413-322-4790 charles.ba...@baystatehealth.org -Original Message- From: Cooper, Brian Sent: Wednesday, February 8, 2023 12:46 PM To: Nancy Schmitt ; Histonet Subject: Re: [Histonet] destain for IHC We used to do this all the time in my old reference lab. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu> From: Nancy Schmitt via Histonet Sent: Wednesday, February 8, 2023 9:29:13 AM To: Histonet Subject: [Histonet] destain for IHC (EXTERNAL EMAIL) CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-boun...@lists.utsouthwestern.edu. Hello- Has anyone had success destaining an H and then running IHC on the same slide? Thanks! Nancy Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://secure-web.cisco.com/18_6RdTZTUgn6UmJHW1nn3zKxUTmGLFAErCLSQhQ4ue4GO5-KLRwTACOn7-7a4Er6_TzsYZZ4UJExAdC7pqHx-oxEawdx8S5CkePiIsj96I7voATRTAboDo7E2qiK9afrwIVXNNuBtZ8SbVV19aaqLNv_C6W09X2oFytd1wMK-vTSuvU_07Lyhi7dgDV_cJFs_J64GiPgdhlO7IfatGhbbblWSNlWIzmpleM9CYPNaXdR-M97emBmUM5kzsc1I7oLqfd8_UkGkyx7mm3fglIjsE-lgwHGa4s8mnu-E2FVrzwZy--we--SKanQ_Ey8mdl4n7ztSRvyeZBx4VChcrr6mD8SvTxjQrDW6C4Az7JEUIYVsLyZRIiBGlYk5KptoQ5LaF68M_Cgw5-SK01KtqhBCoxSljHTzsEdEkA4-SxEHMJWcIpEMD1w6cBoLG0RGerMMY9vRHwAIHAv-xNMXT30yQ/http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet__;JSUlJSUl!!P_vj-BUkwjF5!f6gMMu3epLW9iWatLaXyHemacm0hDOoYOXCaMU5Ijo60vj-bxgHDrpUHZFZGfrf594v9R-jNYLfSmKM-MmAw_V6es7aYOBgKp9eltUXF9cY0L4I$ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. -- Please view our annual report at http://www.bhannualreport.org CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately or by telephone at 413-794- and destroy all copies of this communication and any attachments. For further information regarding Baystate Health's privacy policy, please visit our Internet site at https://www.baystatehealth.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] destain for IHC
We used to do this all the time in my old reference lab. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu> From: Nancy Schmitt via Histonet Sent: Wednesday, February 8, 2023 9:29:13 AM To: Histonet Subject: [Histonet] destain for IHC (EXTERNAL EMAIL) CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-boun...@lists.utsouthwestern.edu. Hello- Has anyone had success destaining an H and then running IHC on the same slide? Thanks! Nancy Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://secure-web.cisco.com/18_6RdTZTUgn6UmJHW1nn3zKxUTmGLFAErCLSQhQ4ue4GO5-KLRwTACOn7-7a4Er6_TzsYZZ4UJExAdC7pqHx-oxEawdx8S5CkePiIsj96I7voATRTAboDo7E2qiK9afrwIVXNNuBtZ8SbVV19aaqLNv_C6W09X2oFytd1wMK-vTSuvU_07Lyhi7dgDV_cJFs_J64GiPgdhlO7IfatGhbbblWSNlWIzmpleM9CYPNaXdR-M97emBmUM5kzsc1I7oLqfd8_UkGkyx7mm3fglIjsE-lgwHGa4s8mnu-E2FVrzwZy--we--SKanQ_Ey8mdl4n7ztSRvyeZBx4VChcrr6mD8SvTxjQrDW6C4Az7JEUIYVsLyZRIiBGlYk5KptoQ5LaF68M_Cgw5-SK01KtqhBCoxSljHTzsEdEkA4-SxEHMJWcIpEMD1w6cBoLG0RGerMMY9vRHwAIHAv-xNMXT30yQ/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] destain for IHC
Hello- Has anyone had success destaining an H and then running IHC on the same slide? Thanks! Nancy Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
