RE: [Histonet] difficult cross section to cut
Louise A product that used to work well for me in the middle ages was called Mollifex came from BDH. Barry From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Louise Renton [louise.ren...@gmail.com] Sent: Wednesday, January 11, 2012 1:37 AM To: Histonet Subject: Re: [Histonet] difficult cross section to cut Hi Peggy 1. I have found that to cut bone the block has to be REALLY cold. Face block with increased knife angle as suggested by Rene, and then place in freezer overnight - then try sectioning 2. Try surface decalcification of your block overnight in whichever soln. you normally use. 3. Section at 4-6 microns 4. You do not say, but high profile disposables work better with bone than low profile 5, failing this, you could try dewaxing and re decalcifying for a few more days good luck On Tue, Jan 10, 2012 at 7:35 PM, DiCarlo, Margaret mdica...@kaleidahealth.org wrote: Histonetters, I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a femur that they think is osteoporotic. After decaling the cross section of the femur in 10% formic acid for 12 days, processing using xylene and embedding in Tissue Prep 2, I have been unsuccessful in attaining a section. The knife just skips over the bone. I have soaked the bone with a 50/50 solution of fabric softener and still can't get a section. Does anyone have any suggestions? Thank you. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 The Keeping You Informed section of Kaleida Health’s website features a wealth of information, stories and pictures about our valued workforce and about the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ist...@kaleidahealth.org or call (716) 859-. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] difficult cross section to cut
Histonetters, For everyone that responded to my email, I was finally able to cut a couple of sections of the cross section of the femur. I surface soaked the block with 50/50 solution of fabric softener for TWO hours, constantly soaking the gauze so it wouldn't dry out, covered it with a plastic container inverted and then iced it for 5 minutes before taking a section. Also, I used a heavy duty long, 25 cm. steel c profile blade to cut the bone. I don't know if it made a difference but I had previously used a shorter, 18.5 cm long steel c knife. Now the trick will be to see if the section stays on when I stain it. Thanks for everyone's help. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of DiCarlo, Margaret Sent: Tuesday, January 10, 2012 12:35 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] difficult cross section to cut Histonetters, I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a femur that they think is osteoporotic. After decaling the cross section of the femur in 10% formic acid for 12 days, processing using xylene and embedding in Tissue Prep 2, I have been unsuccessful in attaining a section. The knife just skips over the bone. I have soaked the bone with a 50/50 solution of fabric softener and still can't get a section. Does anyone have any suggestions? Thank you. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 The Keeping You Informed section of Kaleida Health's website features a wealth of information, stories and pictures about our valued workforce and about the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ist...@kaleidahealth.org or call (716) 859-. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The Keeping You Informed section of Kaleida Healths website features a wealth of information, stories and pictures about our valued workforce and about the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ist...@kaleidahealth.org or call (716) 859-. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] difficult cross section to cut
Histonetters, I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a femur that they think is osteoporotic. After decaling the cross section of the femur in 10% formic acid for 12 days, processing using xylene and embedding in Tissue Prep 2, I have been unsuccessful in attaining a section. The knife just skips over the bone. I have soaked the bone with a 50/50 solution of fabric softener and still can't get a section. Does anyone have any suggestions? Thank you. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 The Keeping You Informed section of Kaleida Healths website features a wealth of information, stories and pictures about our valued workforce and about the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ist...@kaleidahealth.org or call (716) 859-. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] difficult cross section to cut
Try increasing the knife cutting angle. The smaller the angle the more likely the blade is going to skip over the cutting surface. If you are using around 10º, go to 30º angle. René J. --- On Tue, 1/10/12, DiCarlo, Margaret mdica...@kaleidahealth.org wrote: From: DiCarlo, Margaret mdica...@kaleidahealth.org Subject: [Histonet] difficult cross section to cut To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 10, 2012, 12:35 PM Histonetters, I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a femur that they think is osteoporotic. After decaling the cross section of the femur in 10% formic acid for 12 days, processing using xylene and embedding in Tissue Prep 2, I have been unsuccessful in attaining a section. The knife just skips over the bone. I have soaked the bone with a 50/50 solution of fabric softener and still can't get a section. Does anyone have any suggestions? Thank you. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 The Keeping You Informed section of Kaleida Health’s website features a wealth of information, stories and pictures about our valued workforce and about the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ist...@kaleidahealth.org or call (716) 859-. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] difficult cross section to cut
Hi Peggy 1. I have found that to cut bone the block has to be REALLY cold. Face block with increased knife angle as suggested by Rene, and then place in freezer overnight - then try sectioning 2. Try surface decalcification of your block overnight in whichever soln. you normally use. 3. Section at 4-6 microns 4. You do not say, but high profile disposables work better with bone than low profile 5, failing this, you could try dewaxing and re decalcifying for a few more days good luck On Tue, Jan 10, 2012 at 7:35 PM, DiCarlo, Margaret mdica...@kaleidahealth.org wrote: Histonetters, I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a femur that they think is osteoporotic. After decaling the cross section of the femur in 10% formic acid for 12 days, processing using xylene and embedding in Tissue Prep 2, I have been unsuccessful in attaining a section. The knife just skips over the bone. I have soaked the bone with a 50/50 solution of fabric softener and still can't get a section. Does anyone have any suggestions? Thank you. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 The Keeping You Informed section of Kaleida Health’s website features a wealth of information, stories and pictures about our valued workforce and about the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ist...@kaleidahealth.org or call (716) 859-. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
