[Histonet] histomorphometry - clarified
Dear all, just to clarify issues here - i have a histomorphometry analysis system -a very nice one, and i sort of know how to use it - what i DON'T know is what sort of measurements i should be doing. So thanks to all who suggested systems, i'm sorry if my initial e-mail was not all that clear. further commentary on WHAT to measure and what parametres are useful will be welcomed best regards On Fri, Oct 1, 2010 at 12:12 PM, Katie McKinley katie.mckin...@i-path.co.uk wrote: Dear Louise, We have software which not only will allow the easy measurement of slides but also the management of your images. We specialize in whole slide scanning software, We can scan your slides and your images are saved on our network of servers and can be accessed online using secure login details. Because the software is online you can access your images from any location. The scan can be treated like a glass slide. You can zoom in to diagnostic resolution and pan around the whole slide and move through the layers. As well as measurements, you can annotate the slide, build up archives and banks of information and share with colleagues around the world instantly. We are currently in beta testing of our off the shelf imaging analysis software, but we do work on a consultancy basis to create analysis algorithms to suit our clients' needs specifically. Please visit our website for more info. www.i-path.co.uk Kind Regards Katie -Original Message- From: Wagner, Richard [BUL/LAK] [mailto:richard.wag...@buehler.com] Sent: 28 September 2010 21:48 To: louise.ren...@gmail.com Cc: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] histomorphometry Louise: Versatile image analysis software like OmniMet can solve this problem. Powerful digital image acquisition, object and field measurement options, and counting tools are standard features of the software. http://www.buehler.com/productinfo/biomedical/ia.htm Regards, Rick Wagner -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Tuesday, September 28, 2010 8:36 AM To: louise renton Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] histomorphometry At the NSH this year, just this last Sunday in fact, Dr. Tony Villanueva demonstrated this very technique in his workshop. My best advice would be to contact him directly (avillanuev...@cox.net) and see what he can do for you. Another option would be to contact an image analysis vendor like BIOQUANT (nathan...@bioquant.com). Hope this information helps! Regards, Jack On Sep 28, 2010, at 3:25 AM, louise renton louise.ren...@gmail.com wrote: Hi all, I desperately need some advice from an experienced histomorphometristI am trying to translate the old cumbersome visual measurement of using an eypiece grid and doing point counting to an easier computer system - but I am not sure how to do it - any help out there? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histomorphometry
Hi louise! like Yak-Nam WangI do use Image J. It is very easy to use, you can use just a picture and count particles/cells manually or do it automated after defining the size threshold. The last way It will take you a bit more time until you validate the process, but it is no difficult. cheers, sil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] histomorphometry
Hi all, I desperately need some advice from an experienced histomorphometristI am trying to translate the old cumbersome visual measurement of using an eypiece grid and doing point counting to an easier computer system - but I am not sure how to do it - any help out there? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histomorphometry
At the NSH this year, just this last Sunday in fact, Dr. Tony Villanueva demonstrated this very technique in his workshop. My best advice would be to contact him directly (avillanuev...@cox.net) and see what he can do for you. Another option would be to contact an image analysis vendor like BIOQUANT (nathan...@bioquant.com). Hope this information helps! Regards, Jack On Sep 28, 2010, at 3:25 AM, louise renton louise.ren...@gmail.com wrote: Hi all, I desperately need some advice from an experienced histomorphometristI am trying to translate the old cumbersome visual measurement of using an eypiece grid and doing point counting to an easier computer system - but I am not sure how to do it - any help out there? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] histomorphometry
Louise: Versatile image analysis software like OmniMet can solve this problem. Powerful digital image acquisition, object and field measurement options, and counting tools are standard features of the software. http://www.buehler.com/productinfo/biomedical/ia.htm Regards, Rick Wagner -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Tuesday, September 28, 2010 8:36 AM To: louise renton Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] histomorphometry At the NSH this year, just this last Sunday in fact, Dr. Tony Villanueva demonstrated this very technique in his workshop. My best advice would be to contact him directly (avillanuev...@cox.net) and see what he can do for you. Another option would be to contact an image analysis vendor like BIOQUANT (nathan...@bioquant.com). Hope this information helps! Regards, Jack On Sep 28, 2010, at 3:25 AM, louise renton louise.ren...@gmail.com wrote: Hi all, I desperately need some advice from an experienced histomorphometristI am trying to translate the old cumbersome visual measurement of using an eypiece grid and doing point counting to an easier computer system - but I am not sure how to do it - any help out there? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histomorphometry
Hi Louise, If your lab is like other labs and are a bit strapped for money but need basic point counting, you may also be able do this using ImageJ. ImageJ is a free piece of software developed at and provided by NIH. It has grid and point overlays and counting tools. The learning curve isn't very steep, and it's free! http://rsbweb.nih.gov/ij/ There is also a pc version. Good luck Yak-Nam Wang On 9/28/10 1:47 PM, Wagner, Richard [BUL/LAK] richard.wag...@buehler.com wrote: Louise: Versatile image analysis software like OmniMet can solve this problem. Powerful digital image acquisition, object and field measurement options, and counting tools are standard features of the software. http://www.buehler.com/productinfo/biomedical/ia.htm Regards, Rick Wagner -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Tuesday, September 28, 2010 8:36 AM To: louise renton Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] histomorphometry At the NSH this year, just this last Sunday in fact, Dr. Tony Villanueva demonstrated this very technique in his workshop. My best advice would be to contact him directly (avillanuev...@cox.net) and see what he can do for you. Another option would be to contact an image analysis vendor like BIOQUANT (nathan...@bioquant.com). Hope this information helps! Regards, Jack On Sep 28, 2010, at 3:25 AM, louise renton louise.ren...@gmail.com wrote: Hi all, I desperately need some advice from an experienced histomorphometristI am trying to translate the old cumbersome visual measurement of using an eypiece grid and doing point counting to an easier computer system - but I am not sure how to do it - any help out there? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histomorphometry
We use MetaMorph software. It comes with a digital camera for taking the pictures. You can get the software only if you have already the micrioscope and the camera. You can easily measure anything you want, but it is costly. Michelle USC School of Medicine LA CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
