[Histonet] light staining
Hello Histonetters We are having trouble with light staining from the hematoxylin. It is the same lot number we have been in. We changed it out and still the same. All reagent containers were emptied, cleaned and refilled yesterday. We have not changed anything with the timings on our stainer. What am I missing? Thanks for your help Nancy Schmitt United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] light staining
Do you use city water in your wash stations? Have you had more snow/ice than usual? If so the Water Tx Plant is putting more chlorine in the water than usual. This will affect you HE. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] light staining
Check pH of your washing water. pH too low will result in not staining at all. I'd clean the stainer as well. Hello Histonetters We are having trouble with light staining from the hematoxylin. It is the same lot number we have been in. We changed it out and still the same. All reagent containers were emptied, cleaned and refilled yesterday. We have not changed anything with the timings on our stainer. What am I missing? Thanks for your help Nancy Schmitt United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] light staining
I would have a look at the haematoxylin stained section before you differentiate. Does it look OK? If it does then do not differentiate, blue only. (also someone has not accidently put acid in the blueing solution? Check with apH papers). You might try extending the Hx time. If it still looks pale after the eosin, then the acid in the eosin is continuing the differentiation process. Extend your Hx staining time decrease the eosin time. If it is a young Hx then you might find that the staining improves with time (ie it ripens). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Wednesday, 10 February 2010 5:38 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] light staining Hello Histonetters We are having trouble with light staining from the hematoxylin. It is the same lot number we have been in. We changed it out and still the same. All reagent containers were emptied, cleaned and refilled yesterday. We have not changed anything with the timings on our stainer. What am I missing? Thanks for your help Nancy Schmitt United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
