[Histonet] linear stainer hematoxylin staining issues
We have the Hacker linear stainer, and for the past two weeks we had clean, crisp HE staining. However, last Friday, something happened and our HE stains no longer look crisp, but instead have a hazy, unreadable look. Nothing different has been done, and we have troubleshot for every possible scenario. After a clean set up, we noticed that the hematoxylin is not staining very well. We have it set for 2 minutes with agitation for a uniform staining. Hematoxylin is Harris Special (mercury free) from MCC and the eosin is with phloxine b from AMT. After the hematoxylin (2 buckets for total of 4.5 minutes), we use 1.5% acetic acid. It seems that the hematoxylin is not staining well. Any tips? We may need to consider increasing a bucket for hematoxylin? Thanks, Sophia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] linear stainer hematoxylin staining issues
If your acetic acid solution is made in-houseI would suggest remaking it, just in case it was accidently made stronger. Kari Kienitz HT, (ASCP) Histology Laboratory Portland Gastroenterology The Oregon Clinic NE 99th Ave Portland, OR 97220 503.935.8311 kkien...@orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sophia Lin [lins0...@gmail.com] Sent: Wednesday, March 27, 2013 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] linear stainer hematoxylin staining issues We have the Hacker linear stainer, and for the past two weeks we had clean, crisp HE staining. However, last Friday, something happened and our HE stains no longer look crisp, but instead have a hazy, unreadable look. Nothing different has been done, and we have troubleshot for every possible scenario. After a clean set up, we noticed that the hematoxylin is not staining very well. We have it set for 2 minutes with agitation for a uniform staining. Hematoxylin is Harris Special (mercury free) from MCC and the eosin is with phloxine b from AMT. After the hematoxylin (2 buckets for total of 4.5 minutes), we use 1.5% acetic acid. It seems that the hematoxylin is not staining well. Any tips? We may need to consider increasing a bucket for hematoxylin? Thanks, Sophia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] linear stainer hematoxylin staining issues
How do I unsubscribe from Histonet?? Thank you, Arjun On Wed, Mar 27, 2013 at 1:46 PM, Kienitz, Kari kkien...@orclinic.comwrote: If your acetic acid solution is made in-houseI would suggest remaking it, just in case it was accidently made stronger. Kari Kienitz HT, (ASCP) Histology Laboratory Portland Gastroenterology The Oregon Clinic NE 99th Ave Portland, OR 97220 503.935.8311 kkien...@orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you From: histonet-boun...@lists.utsouthwestern.edu [ histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sophia Lin [ lins0...@gmail.com] Sent: Wednesday, March 27, 2013 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] linear stainer hematoxylin staining issues We have the Hacker linear stainer, and for the past two weeks we had clean, crisp HE staining. However, last Friday, something happened and our HE stains no longer look crisp, but instead have a hazy, unreadable look. Nothing different has been done, and we have troubleshot for every possible scenario. After a clean set up, we noticed that the hematoxylin is not staining very well. We have it set for 2 minutes with agitation for a uniform staining. Hematoxylin is Harris Special (mercury free) from MCC and the eosin is with phloxine b from AMT. After the hematoxylin (2 buckets for total of 4.5 minutes), we use 1.5% acetic acid. It seems that the hematoxylin is not staining well. Any tips? We may need to consider increasing a bucket for hematoxylin? Thanks, Sophia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
