RE: [Histonet] neuropath stains
Our immunofluorescence protein antibody stains are automated, but not the routine histochemical stains used for muscle biopsies. Between the importance of maintaining pH levels, incubation temperatures (SDH,NADH,COX, AtPase's, Amyloid, MAD, Myophos, Acid Phos) and the need of a rotator plate (ORO). Really the only options for automation would be the H&E, Gomori Trichrome and PAS. Sarah E. Lewis HT, ASCP Laboratory Coordinator The Research Institute at Nationwide Children's Hospital Center for Gene Therapy Neuromuscular Division Rm WA3110 (614)722-2204 -Original Message- From: Nails, Felton [mailto:flna...@texaschildrens.org] Sent: Wednesday, November 13, 2013 5:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] neuropath stains Are the basic muscle and nerve biopsy stains being performed manually or has someone automated these stains? __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] neuropath stains
So far when I ask about neuro stains being automated by any company the answer is no. The volume clinically is not high enough and the times required would tie the stainers up from hours to overnight in for some steps. It would almost require a dedicated stainer for neuro only and would be very expensive. We do ours manually. Pam Marcum UAMS - Original Message - From: "Felton Nails" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, November 13, 2013 4:46:04 PM Subject: [Histonet] neuropath stains Are the basic muscle and nerve biopsy stains being performed manually or has someone automated these stains? __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] neuropath stains
Are the basic muscle and nerve biopsy stains being performed manually or has someone automated these stains? __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
