Re: [Histonet] number of slides

2010-03-25 Thread Anne van Binsbergen
Here is our basic microtomy protocol:

BMT - 3 HEs, each has a ribbon, plus PAS, Retic, Perls
Liver bx - 3 HEs, each has a ribbon plus Retic, Trichrome, Perls,
Renal bx - 4 HEs, each has a short ribbon plus PAS, PMS, trichrome - on
slides with gloms
Breast bx - 3 HEs, each has a ribbon
Derm bx - 4 HEs, each has a ribbon, one Path likes AP PAS on all punch bx's
GIT bx - 3 HEs, each has a ribbon, plus HP

all other small bx's get 3 HEs, each with a ribbon

and then we wait for the orders for levels and deepers and..and...and

AbuDhabiAnnie



On 25 March 2010 00:19, hymclab hymclab.hymc...@ministryhealth.org wrote:

 We follow the same practice as Susan.  Our Pathologists would rather have
 more than less!!!

 Dawn

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
 Sent: Tuesday, March 23, 2010 5:08 PM
 To: anita dudley
 Cc: histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] number of slides

 We do three levels on all diagnostic biopsies. Liver and Kidney also get
 upfront special stains, as do gastric biopsies (h pylori) We did cut down on
 extra unstained slides since we were discarding most of them. As far as
 stopping levels, my pathologist thinks it goes against standard of care. As
 for prostate biopsies, they are so small any more, that we are thinking o
 cutting 4 slides staining 1and 4 for HE and holding 2 and 3 for possible
 IHC. We are finding that when we have to go back there is minimal tumor for
 IHC demonstration.

 Susan T. Paturzo
 Thomas Jefferson University Hospital


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-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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RE: [Histonet] number of slides

2010-03-24 Thread Tom McNemar
We do 2 levels on all GIs, 3 on all needle bx, 3 on cervical, and 2 on bone 
marrows.  We also do special stains up front on the bone marrows, H Pyloris, 
prostates, etc.

We tend to cut a ton of extra slides that we just throw away.  I don't like it 
but I don't think there's any way around it.  Probably like most places, we get 
more and more smaller and smaller biopsies so it's better to keep a few extras 
than to try to go back later.



Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Tuesday, March 23, 2010 6:08 PM
To: anita dudley
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] number of slides

We do three levels on all diagnostic biopsies. Liver and Kidney also get 
upfront special stains, as do gastric biopsies (h pylori)
We did cut down on extra unstained slides since we were discarding most of 
them. As far as stopping levels, my pathologist thinks it goes against standard
of care. As for prostate biopsies, they are so small any more, that we are 
thinking o cutting 4 slides staining 1and 4 for HE and holding 2 and 3 for 
possible IHC. We
are finding that when we have to go back there is minimal tumor for IHC 
demonstration.

Susan T. Paturzo
Thomas Jefferson University Hospital


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RE: [Histonet] number of slides

2010-03-24 Thread Podawiltz, Thomas
We basically do the same at Tom's lab, the only exception is we file the 
unstained slides with the stained slides. Especially with the FNA biopsies. We 
have had to pull the unstained slide to send out for IHC's. 


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar 
[tmcne...@lmhealth.org]
Sent: Wednesday, March 24, 2010 6:11 AM
To: Sue; anita dudley
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] number of slides

We do 2 levels on all GIs, 3 on all needle bx, 3 on cervical, and 2 on bone 
marrows.  We also do special stains up front on the bone marrows, H Pyloris, 
prostates, etc.

We tend to cut a ton of extra slides that we just throw away.  I don't like it 
but I don't think there's any way around it.  Probably like most places, we get 
more and more smaller and smaller biopsies so it's better to keep a few extras 
than to try to go back later.



Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Tuesday, March 23, 2010 6:08 PM
To: anita dudley
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] number of slides

We do three levels on all diagnostic biopsies. Liver and Kidney also get 
upfront special stains, as do gastric biopsies (h pylori)
We did cut down on extra unstained slides since we were discarding most of 
them. As far as stopping levels, my pathologist thinks it goes against standard
of care. As for prostate biopsies, they are so small any more, that we are 
thinking o cutting 4 slides staining 1and 4 for HE and holding 2 and 3 for 
possible IHC. We
are finding that when we have to go back there is minimal tumor for IHC 
demonstration.

Susan T. Paturzo
Thomas Jefferson University Hospital


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recipient, be aware that any disclosure, copying, distribution or use of the 
contents of this e-mail and attachments is prohibited. If you have received 
this in error, please advise the sender by reply e-mail and delete the message 
immediately. You may also contact the LMH Process Improvement Center at 
740-348-4061. E-mail transmissions cannot be guaranteed to be secure or 
error-free as information could be intercepted, corrupted, lost, destroyed, 
arrive late or incomplete, or contain viruses. The sender therefore does not 
accept liability for any errors or omissions in the contents of this message, 
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RE: [Histonet] number of slides

2010-03-24 Thread hymclab
We follow the same practice as Susan.  Our Pathologists would rather have more 
than less!!!

Dawn

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Tuesday, March 23, 2010 5:08 PM
To: anita dudley
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] number of slides

We do three levels on all diagnostic biopsies. Liver and Kidney also get 
upfront special stains, as do gastric biopsies (h pylori) We did cut down on 
extra unstained slides since we were discarding most of them. As far as 
stopping levels, my pathologist thinks it goes against standard of care. As for 
prostate biopsies, they are so small any more, that we are thinking o cutting 4 
slides staining 1and 4 for HE and holding 2 and 3 for possible IHC. We are 
finding that when we have to go back there is minimal tumor for IHC 
demonstration.

Susan T. Paturzo
Thomas Jefferson University Hospital


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[Histonet] number of slides

2010-03-23 Thread anita dudley

just wondering what others were doing with colon bxs. embs, eccs.  do you use 
one slide or cut 2 to 3 slides per block?  lungs and livers too.  thanks, we 
are thinking of going to one slide.  

 

anita dudley

providence hosp.

mobile alabama
  
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Re: [Histonet] number of slides

2010-03-23 Thread Sue
We do three levels on all diagnostic biopsies. Liver and Kidney also get 
upfront special stains, as do gastric biopsies (h pylori) 
We did cut down on extra unstained slides since we were discarding most of 
them. As far as stopping levels, my pathologist thinks it goes against standard 
of care. As for prostate biopsies, they are so small any more, that we are 
thinking o cutting 4 slides staining 1and 4 for HE and holding 2 and 3 for 
possible IHC. We 
are finding that when we have to go back there is minimal tumor for IHC 
demonstration. 

Susan T. Paturzo 
Thomas Jefferson University Hospital 


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Re: [Histonet] number of slides

2010-03-23 Thread Malika Benatti
Routinely

Gastric bx  upper / rt colon / lt colon one rubbon of 4 sections per slides
Liver HE @ level 1/2/3 and liver specials, AE1 IHC
Renal Biopsy (Native and Transplant) 24 slides inc HE/Renal Special/ IHC and
USS
TransBronchial BX HE @ level 1/2/3 and lung special
BMT HE, retic and IHC for NBLX case
Endomyocardial BX, HE @level 1/2/3 EVG/MT and C4d IHC
Vascular Malformation HE and IHC
Surgical Heart (11 blocks) HE, EVG on all Blocks
Cornea Button HE, PAS, AB, Congo red
All Tumour BX HE, and 10 USS for IHC
PM Brain HE/LFB on all blocks
PM Heart HE/EVG on all blocks
PM Lung HE/EVG/PERLS on all blocks

Working in paediatric I have not seen a prostate of a breast for the past 6
years, but my old lab had between 4 and 6 blocks per prostate or breast
tissue and were cutting HE at level 1/2/3 and picking up 4 USS between level
for further IHC.


Malika

Malika Benatti
Specialist Biomedical Scientist
Great Ormond Street Hospital for Children


On Tue, Mar 23, 2010 at 10:08 PM, Sue suetp...@comcast.net wrote:

 We do three levels on all diagnostic biopsies. Liver and Kidney also get
 upfront special stains, as do gastric biopsies (h pylori)
 We did cut down on extra unstained slides since we were discarding most of
 them. As far as stopping levels, my pathologist thinks it goes against
 standard
 of care. As for prostate biopsies, they are so small any more, that we are
 thinking o cutting 4 slides staining 1and 4 for HE and holding 2 and 3 for
 possible IHC. We
 are finding that when we have to go back there is minimal tumor for IHC
 demonstration.

 Susan T. Paturzo
 Thomas Jefferson University Hospital


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