Re: [Histonet] processing v-e-r-y tiny samples
Shandon - or whatever they're called this week- sell nylon mesh biopsy bags. These are flat bags, sealed on 3 sides. I have found that if you cut one corner off (ie, a oblong piece with 2 sides still sealed) just a tiny bit bigger than the cassette, you can open the bag, like a book, insert the sample and flatten the bag over the specimen and place it snug and flat in the bottom of the casette. It sems to stay like that through processing (esp if kept the cassette is kept flat in the basket), and is easy to locate against the mesh for embedding. On 3/19/09, Andrea Grantham algra...@u.arizona.edu wrote: Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi . : Andrea Grantham, HT(ASCP) Dept. of Cell Biology Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415)Tucson, AZ 85724-5044USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...: http://www.cba.arizona.edu/histology-lab.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] processing v-e-r-y tiny samples
Hi Andi, Happy Friday! I know there's been a Histogel backlash of late, but I would still recommend trying it. I haven't had any problems. We use it for scant neuro specimens every day. We also have used H.G. for mouse sural nerve biopsies that needed to retain orientation (very tiny). If you do use Histogel, do not put it in a histoscreen or biopsy cassette and be sure to put it on a process that's long enough. People often use the cassette/process based on the size of the tissue, not of the Histogel block. I'd ask thermo for a sample of H.G. and try it. If you need any Histogel help, let me know. I'm a huge fan! Have a great day, Jennifer Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -Original Message- From: Bryan Watson [mailto:bryan.wat...@parkview.com] Sent: Thursday, March 19, 2009 3:39 PM To: histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: Re: [Histonet] processing v-e-r-y tiny samples I may never complain about tiny GI or bronch biopsies ever again! Andrea Grantham algra...@u.arizona.edu 3/19/2009 11:51 AM Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi . : Andrea Grantham, HT(ASCP) Dept. of Cell Biology Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415)Tucson, AZ 85724-5044USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...: http://www.cba.arizona.edu/histology-lab.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] processing v-e-r-y tiny samples
Careful - you may be close to the mark. Thermo laid off about 75 people here in Madison last week. Claire Shandon - or whatever they're called this week- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] processing v-e-r-y tiny samples
Well, you could always hand process them in test tubes. Jerry Ricks Research Scientist University of Washington Department of Pathology Date: Thu, 19 Mar 2009 16:39:14 -0400 From: bryan.wat...@parkview.com To: histonet@lists.utsouthwestern.edu; algra...@u.arizona.edu Subject: Re: [Histonet] processing v-e-r-y tiny samples CC: I may never complain about tiny GI or bronch biopsies ever again! Andrea Grantham algra...@u.arizona.edu 3/19/2009 11:51 AM Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi . : Andrea Grantham, HT(ASCP) Dept. of Cell Biology Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415)Tucson, AZ 85724-5044USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...: http://www.cba.arizona.edu/histology-lab.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Internet Explorer 8 – Now Available. Faster, safer, easier. http://clk.atdmt.com/MRT/go/141323790/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] processing v-e-r-y tiny samples
Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi . : Andrea Grantham, HT(ASCP) Dept. of Cell Biology Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415)Tucson, AZ 85724-5044USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...: http://www.cba.arizona.edu/histology-lab.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] processing v-e-r-y tiny samples
Andi only gets the fun stuff!!! Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bryan Watson Sent: Thursday, March 19, 2009 4:39 PM To: histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: Re: [Histonet] processing v-e-r-y tiny samples I may never complain about tiny GI or bronch biopsies ever again! Andrea Grantham algra...@u.arizona.edu 3/19/2009 11:51 AM Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi . : Andrea Grantham, HT(ASCP) Dept. of Cell Biology Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415)Tucson, AZ 85724-5044USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...: http://www.cba.arizona.edu/histology-lab.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
