Re: [Histonet] protocol for in situ RT_PCR
hi could you add me on too pelase? On Mon, Aug 15, 2011 at 11:09 PM, Barone, Carol cbar...@nemours.org wrote: Anyone got a hot protocol for In situ RT-PCR? Haven't done one in 5 years (on my Perk and Elmer)...Know there must be some great new reagents and kits out there...opinions? Send to cbar...@nemours.org. Thx CB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] protocol for in situ RT_PCR
Hello, Does anyone have used alcoholic zinc-formalin to fix mouse tissues? We tried a few times with this fixative for immunohistochemical staining, it did not work, but when switched back to 4% paraformaldehyde, the immunostaining worked. We like alcoholic zinc-formalin as it gives better morphology. Any suggestions? Thanks, Frank -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Louise Renton Sent: Tuesday, August 16, 2011 4:17 AM To: Barone, Carol; Histonet Subject: Re: [Histonet] protocol for in situ RT_PCR hi could you add me on too pelase? On Mon, Aug 15, 2011 at 11:09 PM, Barone, Carol cbar...@nemours.org wrote: Anyone got a hot protocol for In situ RT-PCR? Haven't done one in 5 years (on my Perk and Elmer)...Know there must be some great new reagents and kits out there...opinions? Send to cbar...@nemours.org. Thx CB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] protocol for in situ RT_PCR
I've used 10% zinc buffered formalin from Anatech (no alcohol) on mouse bones with good results. It's possible that the alcohol is denaturing your antigens of interest. Is there a reason you need to use alcoholic formalin? Adam On Tue, Aug 16, 2011 at 7:45 AM, Liang, Frank fli...@wellstatoc.com wrote: Hello, Does anyone have used alcoholic zinc-formalin to fix mouse tissues? We tried a few times with this fixative for immunohistochemical staining, it did not work, but when switched back to 4% paraformaldehyde, the immunostaining worked. We like alcoholic zinc-formalin as it gives better morphology. Any suggestions? Thanks, Frank -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Louise Renton Sent: Tuesday, August 16, 2011 4:17 AM To: Barone, Carol; Histonet Subject: Re: [Histonet] protocol for in situ RT_PCR hi could you add me on too pelase? On Mon, Aug 15, 2011 at 11:09 PM, Barone, Carol cbar...@nemours.org wrote: Anyone got a hot protocol for In situ RT-PCR? Haven't done one in 5 years (on my Perk and Elmer)...Know there must be some great new reagents and kits out there...opinions? Send to cbar...@nemours.org. Thx CB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] protocol for in situ RT_PCR
Anyone got a hot protocol for In situ RT-PCR? Haven't done one in 5 years (on my Perk and Elmer)...Know there must be some great new reagents and kits out there...opinions? Send to cbar...@nemours.org. Thx CB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
