[Histonet] uh...
So I just stained a group of slides all at the same time with the same conditions. About 40 of 150 the eosin looks like it is bleeding out of the sections...this has never happened before? What could be the cause and how do I fix it? Everything is as normal, but again I am being forced to use the crappy stuff called clear rite. Could this be the cause of the bleeding? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] uh...
Sounds like your dehydrating alcohol is not dehydrating completely. I would switch out the 100%'s and the clearite. Either they got wet during the course of the day or someone made an oops when changing the stain line. Hope that helps. Will Sent from my iPhone On Apr 7, 2011, at 2:06 PM, sgoe...@mirnarx.com wrote: So I just stained a group of slides all at the same time with the same conditions. About 40 of 150 the eosin looks like it is bleeding out of the sections...this has never happened before? What could be the cause and how do I fix it? Everything is as normal, but again I am being forced to use the crappy stuff called clear rite. Could this be the cause of the bleeding? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] uh...
Oh yes, how to fix it. Hydrate through clearite, 100% ETOH, 95% ETOH, and water. Spend an extra 5 mins in running tap water -- that should wash out most of the eosin. Then counterstain again with eosin. Dehydrate with the clean ETOHs and Clearite. That should do it. Another possibility I just thought of, I never used clearite for coverslipping, even in labs that primarily used clearite -- I always finished up in Xylene. Will --- On Thu, 4/7/11, William Chappell cha...@yahoo.com wrote: From: William Chappell cha...@yahoo.com Subject: Re: [Histonet] uh... To: sgoe...@mirnarx.com sgoe...@mirnarx.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Thursday, April 7, 2011, 2:11 PM Sounds like your dehydrating alcohol is not dehydrating completely. I would switch out the 100%'s and the clearite. Either they got wet during the course of the day or someone made an oops when changing the stain line. Hope that helps. Will Sent from my iPhone On Apr 7, 2011, at 2:06 PM, sgoe...@mirnarx.com wrote: So I just stained a group of slides all at the same time with the same conditions. About 40 of 150 the eosin looks like it is bleeding out of the sections...this has never happened before? What could be the cause and how do I fix it? Everything is as normal, but again I am being forced to use the crappy stuff called clear rite. Could this be the cause of the bleeding? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] uh...
We had the same problem years ago using Clear-rite. It turned out to be the mounting medium was not compatible. We use Clear-rite routinely and do not have problems with the eosin bleeding. We use Permount or on our coverslipper we use Sakura brand. Jennifer sgoe...@mirnarx.com Sent by: histonet-boun...@lists.utsouthwestern.edu 04/07/2011 02:09 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] uh... So I just stained a group of slides all at the same time with the same conditions. About 40 of 150 the eosin looks like it is bleeding out of the sections...this has never happened before? What could be the cause and how do I fix it? Everything is as normal, but again I am being forced to use the crappy stuff called clear rite. Could this be the cause of the bleeding? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
