Re: [Histonet] Blades for cutting resin on a microtome
Veronique, May I ask what type of specimen is embedded into the JB-4 resin? Nevertheless, you should be able to cut these blocks using a tungsten-carbide knife. While there are a few vendors out there that sell these knives, in my laboratory I personally use knives re-sharpened by Delaware Diamond Knives (DDK). Please feel free to message me privately if you need further assistance as I have been working with resin embedded specimens for over 17 years. I will also encourage you to reach out to Sarah Mack as she is the new Hard Tissue Committee Chairperson for the National Society for Histotechnology. You can find her contact information and additional information about the committee by visiting www.nsh.org! Best Regards, Jack Ratliff On Sep 12, 2014, at 9:32 AM, Véronique Barrès veronique.bar...@gmail.com wrote: Happy Friday Histonetters! I am working on a histology platform in a research center and someone came to me last week and asked to cut blocs of resin (JB-4 resin) on the microtome. I never cut anything else than paraffin, so I was wondering if some of you had advices for me? They never did it neither and took their protocol in a paper where it was said that we should use disposable glass knife instead of standard metal blades. Are any of you ever used those knife? Where do you buy them? We have an old Leica RM2125. Thanks for your advices! Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Blades for cutting resin on a microtome
I've only cut resin with a glass or diamond knife in an ultramicrotome. If you are attempting to do it in a regular microtome, you would need a special blade holder. I don't know if any microtome manufactures make glass knife holders. You make the glass blades yourself using special glass. Here is a link to the glass strips: http://www.sigmaaldrich.com/catalog/product/sigma/g2528?lang=enregion=US Here is a cheap jig and diamond glass cutters it make the knifes: https://www.emsdiasum.com/microscopy/products/preparation/glassknife.aspx I've never made glass knives by hand using a hand held diamond cutter and jigs. I imagine that it would take practice. I've only used a specialized maker: https://www.emsdiasum.com/microscopy/products/histology/tissue_stainer.aspx You paint a bit of nail polish underneath the glass edge and put a bit of distilled water on the edge. You then section the block floating the sections on the water. Use an eyelash manipulator to pick up the 5um thick sections and place on a bubble of water on the slide. Evaporate the water droplet on the slide. If you've done it right, the sections won't look like origami. If it does, then practice until it doesn't. Gerry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès Sent: Friday, September 12, 2014 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades for cutting resin on a microtome Happy Friday Histonetters! I am working on a histology platform in a research center and someone came to me last week and asked to cut blocs of resin (JB-4 resin) on the microtome. I never cut anything else than paraffin, so I was wondering if some of you had advices for me? They never did it neither and took their protocol in a paper where it was said that we should use disposable glass knife instead of standard metal blades. Are any of you ever used those knife? Where do you buy them? We have an old Leica RM2125. Thanks for your advices! Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Blades for cutting resin on a microtome
Yes brightinstruments, com make glass knife holder and tungsten carbide tipped knives for microtomes, KR,Alan Bright Sent from my iPhone On 12 Sep 2014, at 15:49, Keyser Gerald T gkey...@uwhealth.org wrote: I've only cut resin with a glass or diamond knife in an ultramicrotome. If you are attempting to do it in a regular microtome, you would need a special blade holder. I don't know if any microtome manufactures make glass knife holders. You make the glass blades yourself using special glass. Here is a link to the glass strips: http://www.sigmaaldrich.com/catalog/product/sigma/g2528?lang=enregion=US Here is a cheap jig and diamond glass cutters it make the knifes: https://www.emsdiasum.com/microscopy/products/preparation/glassknife.aspx I've never made glass knives by hand using a hand held diamond cutter and jigs. I imagine that it would take practice. I've only used a specialized maker: https://www.emsdiasum.com/microscopy/products/histology/tissue_stainer.aspx You paint a bit of nail polish underneath the glass edge and put a bit of distilled water on the edge. You then section the block floating the sections on the water. Use an eyelash manipulator to pick up the 5um thick sections and place on a bubble of water on the slide. Evaporate the water droplet on the slide. If you've done it right, the sections won't look like origami. If it does, then practice until it doesn't. Gerry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès Sent: Friday, September 12, 2014 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades for cutting resin on a microtome Happy Friday Histonetters! I am working on a histology platform in a research center and someone came to me last week and asked to cut blocs of resin (JB-4 resin) on the microtome. I never cut anything else than paraffin, so I was wondering if some of you had advices for me? They never did it neither and took their protocol in a paper where it was said that we should use disposable glass knife instead of standard metal blades. Are any of you ever used those knife? Where do you buy them? We have an old Leica RM2125. Thanks for your advices! Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Blades for cutting resin on a microtome
I used to have a triangle glass knife holder insert for my Leica microtome or I would use the tungsten carbide knives. It depends on what you are cutting. if it is calcified bone the glass knives scratch too much and they are only 1/2 inch wide so you have to cut smaller soft tissues with them. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net From: abri...@brightinstruments.com Date: Fri, 12 Sep 2014 17:32:47 +0100 To: gkey...@uwhealth.org Subject: Re: [Histonet] Blades for cutting resin on a microtome CC: histonet@lists.utsouthwestern.edu; veronique.bar...@gmail.com; exp...@brightinstruments.com Yes brightinstruments, com make glass knife holder and tungsten carbide tipped knives for microtomes, KR,Alan Bright Sent from my iPhone On 12 Sep 2014, at 15:49, Keyser Gerald T gkey...@uwhealth.org wrote: I've only cut resin with a glass or diamond knife in an ultramicrotome. If you are attempting to do it in a regular microtome, you would need a special blade holder. I don't know if any microtome manufactures make glass knife holders. You make the glass blades yourself using special glass. Here is a link to the glass strips: http://www.sigmaaldrich.com/catalog/product/sigma/g2528?lang=enregion=US Here is a cheap jig and diamond glass cutters it make the knifes: https://www.emsdiasum.com/microscopy/products/preparation/glassknife.aspx I've never made glass knives by hand using a hand held diamond cutter and jigs. I imagine that it would take practice. I've only used a specialized maker: https://www.emsdiasum.com/microscopy/products/histology/tissue_stainer.aspx You paint a bit of nail polish underneath the glass edge and put a bit of distilled water on the edge. You then section the block floating the sections on the water. Use an eyelash manipulator to pick up the 5um thick sections and place on a bubble of water on the slide. Evaporate the water droplet on the slide. If you've done it right, the sections won't look like origami. If it does, then practice until it doesn't. Gerry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès Sent: Friday, September 12, 2014 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades for cutting resin on a microtome Happy Friday Histonetters! I am working on a histology platform in a research center and someone came to me last week and asked to cut blocs of resin (JB-4 resin) on the microtome. I never cut anything else than paraffin, so I was wondering if some of you had advices for me? They never did it neither and took their protocol in a paper where it was said that we should use disposable glass knife instead of standard metal blades. Are any of you ever used those knife? Where do you buy them? We have an old Leica RM2125. Thanks for your advices! Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
