RE: [Histonet] GMS-Fungus on nails
Prepare a 10% solution of Titebond II premium wood glue (found in most hardware stores). Dip the slide in the solution just before mounting the section on the slide. Let the slide dry and stain away. Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Friday, July 24, 2009 11:31 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS-Fungus on nails We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the slide. We've tried baking for long periods, pre-treating in formalin, using silane slides, with no luck. Even when the nails cut relatively easily we still lose it, and end up running several GMS stains before we might get a speck or two of tissue we can look at. We use the Artisan stainer for our specials. We are really not interested in performing the GMS manually, due to volume and turn around time restrictions. Any suggestions? Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] GMS-Fungus on nails
We use Albumin Solution from Sigma (A7034-10ml) on our slides. We stain on the Artisan and or the Ventana stainer as well. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Friday, July 24, 2009 9:31 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS-Fungus on nails We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the slide. We've tried baking for long periods, pre-treating in formalin, using silane slides, with no luck. Even when the nails cut relatively easily we still lose it, and end up running several GMS stains before we might get a speck or two of tissue we can look at. We use the Artisan stainer for our specials. We are really not interested in performing the GMS manually, due to volume and turn around time restrictions. Any suggestions? Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] GMS-Fungus on nails
For really difficult tissues such as bone, cartilage and perhaps nails I make a 5% solution of elmers glue in dih20 and dip regular uncoated slides in the glue, let them air dry. Use the glue coated slides to pick up sections, just dipping in the water bath seems to activate the glue enough to help the sections stay on during staining. Dry the sections picked up on the glue coated slides in a 60dc oven overnight if possible or I use a slide warmer and lay them flat and dry at 55dc (that is as high as my slides warmer goes) for several hours. Good luck, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 email pru...@ihctech.net website www.ihctech.net IHC Resource Group www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Friday, July 24, 2009 9:31 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS-Fungus on nails We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the slide. We've tried baking for long periods, pre-treating in formalin, using silane slides, with no luck. Even when the nails cut relatively easily we still lose it, and end up running several GMS stains before we might get a speck or two of tissue we can look at. We use the Artisan stainer for our specials. We are really not interested in performing the GMS manually, due to volume and turn around time restrictions. Any suggestions? Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] GMS-Fungus on nails
Just wondering why you use Titebond II? We use plain old Elmer's Glue. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! jstaruk jsta...@masshistology.com 7/24/2009 11:41 AM Prepare a 10% solution of Titebond II premium wood glue (found in most hardware stores). Dip the slide in the solution just before mounting the section on the slide. Let the slide dry and stain away. Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Friday, July 24, 2009 11:31 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS-Fungus on nails We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the slide. We've tried baking for long periods, pre-treating in formalin, using silane slides, with no luck. Even when the nails cut relatively easily we still lose it, and end up running several GMS stains before we might get a speck or two of tissue we can look at. We use the Artisan stainer for our specials. We are really not interested in performing the GMS manually, due to volume and turn around time restrictions. Any suggestions? Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] GMS-Fungus on nails
We soak our nails in 20% sodium hydroxide for at least an hour, rinse in running tap water for a few minutes before we place the cassettes on the tissue processor. We use positive charged slides, heat slides in 80 degree oven for 20 minutes and perform a PAS/fungus on the slides. The PAS is a lot more gentler on the tissue that the GMS. We are the toenail lab for the U.S. Air Force , Joe The Toe - Original Message - From: jstaruk jsta...@masshistology.com To: 'Clare Thornton' cthorn...@dahlchase.com; histonet@lists.utsouthwestern.edu Sent: Friday, July 24, 2009 10:41 AM Subject: RE: [Histonet] GMS-Fungus on nails Prepare a 10% solution of Titebond II premium wood glue (found in most hardware stores). Dip the slide in the solution just before mounting the section on the slide. Let the slide dry and stain away. Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Friday, July 24, 2009 11:31 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS-Fungus on nails We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the slide. We've tried baking for long periods, pre-treating in formalin, using silane slides, with no luck. Even when the nails cut relatively easily we still lose it, and end up running several GMS stains before we might get a speck or two of tissue we can look at. We use the Artisan stainer for our specials. We are really not interested in performing the GMS manually, due to volume and turn around time restrictions. Any suggestions? Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
