RE: [Histonet] Help With Hemo Fading
I agree.. Also you're not storing them in sunlight are you? Silly question I know. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 02 July 2009 20:28 To: histonet@lists.utsouthwestern.edu; sr...@aol.com Subject: Re: [Histonet] Help With Hemo Fading The fading most probably is caused by acid in the permanent slide, probably because the sections were passed through the alcohols very quickly after the acid differentiation, or they stayed little time in tap water after differentiation or no bluing agent was used. It is unlikely that the mounting medium is acidic, although that could also be the cause also. An acid environment over the cover slipped section is the most probable culprit for the henatoxylin fading. Check the staining protocol. René J. --- On Thu, 7/2/09, sr...@aol.com sr...@aol.com wrote: From: sr...@aol.com sr...@aol.com Subject: [Histonet] Help With Hemo Fading To: histonet@lists.utsouthwestern.edu Date: Thursday, July 2, 2009, 2:42 PM Hello everyone, ? We are having problems with short-term hematoxylin fading and loss of detail. The pathologist is freaking out! I've seen hemo fade over a long period of time but not in a matter of a few months. Slides from one year ago are really bad. ? I've been out of the business for a number of years and in the interim much has changed including reagents. These are GI tract biopsies processed by microwave. ? Any thoughts at all? ? Thanks! Marg ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help With Hemo Fading
Even long term exposure to indoor lighting (fluorescent type) will fade sectiions. We work in a building where ther lights are permanently on. If we want to keep our sections fresh and helathy, they get covered up as soon as . best regards On Fri, Jul 3, 2009 at 9:09 AM, Kemlo Rogerson kemlo.roger...@waht.swest.nhs.uk wrote: I agree.. Also you're not storing them in sunlight are you? Silly question I know. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 02 July 2009 20:28 To: histonet@lists.utsouthwestern.edu; sr...@aol.com Subject: Re: [Histonet] Help With Hemo Fading The fading most probably is caused by acid in the permanent slide, probably because the sections were passed through the alcohols very quickly after the acid differentiation, or they stayed little time in tap water after differentiation or no bluing agent was used. It is unlikely that the mounting medium is acidic, although that could also be the cause also. An acid environment over the cover slipped section is the most probable culprit for the henatoxylin fading. Check the staining protocol. René J. --- On Thu, 7/2/09, sr...@aol.com sr...@aol.com wrote: From: sr...@aol.com sr...@aol.com Subject: [Histonet] Help With Hemo Fading To: histonet@lists.utsouthwestern.edu Date: Thursday, July 2, 2009, 2:42 PM Hello everyone, ? We are having problems with short-term hematoxylin fading and loss of detail. The pathologist is freaking out! I've seen hemo fade over a long period of time but not in a matter of a few months. Slides from one year ago are really bad. ? I've been out of the business for a number of years and in the interim much has changed including reagents. These are GI tract biopsies processed by microwave. ? Any thoughts at all? ? Thanks! Marg ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help With Hemo Fading
The fading most probably is caused by acid in the permanent slide, probably because the sections were passed through the alcohols very quickly after the acid differentiation, or they stayed little time in tap water after differentiation or no bluing agent was used. It is unlikely that the mounting medium is acidic, although that could also be the cause also. An acid environment over the cover slipped section is the most probable culprit for the henatoxylin fading. Check the staining protocol. René J. --- On Thu, 7/2/09, sr...@aol.com sr...@aol.com wrote: From: sr...@aol.com sr...@aol.com Subject: [Histonet] Help With Hemo Fading To: histonet@lists.utsouthwestern.edu Date: Thursday, July 2, 2009, 2:42 PM Hello everyone, ? We are having problems with short-term hematoxylin fading and loss of detail. The pathologist is freaking out! I've seen hemo fade over a long period of time but not in a matter of a few months. Slides from one year ago are really bad. ? I've been out of the business for a number of years and in the interim much has changed including reagents. These are GI tract biopsies processed by microwave. ? Any thoughts at all? ? Thanks! Marg ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Help With Hemo Fading
messy that it had been recommended that I submit to a bone marrow exam to determine my proximity to death. I quietly demanded to know how the differential was done. The answer? The Coulter counter! I quietly agreed to a bone marrow exam AFTER a manual differential was performed. Later, the hematologist only wanted to know how I knew. I told her that while working in a path lab at night during college (I know I wasn't 'certified'!!!), I had compared myself to the 'Counter' and I was frankly more consistent. I admitted that my test was not statistical, but I had real confidence in the capacity of the eye and little in the photometer looking thru glass. Machines may be necessary to do the work, but they do not clean themselves and they do not cry out when the 95% ethanol has become 87%. With Coplin jars, variables were easy to manage. They were more difficult the more we tried to do multiples of specimens. This should be obvious in spite of the fact that automation is practical and manual is not - even though sometimes necessary. When I started in histology, I began at the beginning of the Paraplast revolution, yet there were still those who formulated their own paraffin for the part of the planet in which they worked. Sorry for the late-night babble, but I have vented stored up compulsion. Cheers, Fred Monson P.S. Those who are required to volunteer do NOT. Those who require others to volunteer fail. Every tax is a means by which a government forces a citizen to pay for the vacations of those who work for the government. When the USA has a public health plan, every union will be forced to accept it by the employer, and all those who want something better will be required to pay for the full load. Government plan - 'free', all others - cost extra. That's competition? Why won't the insurance companies want to give 'free' public health care to their employees? Their own actuaries will show them the way. Three of four wars prosecuted by the United States during the 20th century were begun under democratically elected Presidents. One was fought entirely by volunteers. During only two of the four have a majority of citizens, at one time or another, wished the U.S.A. to lose(???). Three cheers for the Draft! Beware the resolve of an elected President not to lose. Frederick C. Monson, PhD Technical Director, CMIRT West Chester University West Chester, PA, 19383 610-738-0437 http://cmirt.wcupa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Thu 7/2/2009 3:27 PM To: histonet@lists.utsouthwestern.edu; sr...@aol.com Subject: Re: [Histonet] Help With Hemo Fading The fading most probably is caused by acid in the permanent slide, probably because the sections were passed through the alcohols very quickly after the acid differentiation, or they stayed little time in tap water after differentiation or no bluing agent was used. It is unlikely that the mounting medium is acidic, although that could also be the cause also. An acid environment over the cover slipped section is the most probable culprit for the henatoxylin fading. Check the staining protocol. René J. --- On Thu, 7/2/09, sr...@aol.com sr...@aol.com wrote: From: sr...@aol.com sr...@aol.com Subject: [Histonet] Help With Hemo Fading To: histonet@lists.utsouthwestern.edu Date: Thursday, July 2, 2009, 2:42 PM Hello everyone, ? We are having problems with short-term hematoxylin fading and loss of detail. The pathologist is freaking out! I've seen hemo fade over a long period of time but not in a matter of a few months. Slides from one year ago are really bad. ? I've been out of the business for a number of years and in the interim much has changed including reagents. These are GI tract biopsies processed by microwave. ? Any thoughts at all? ? Thanks! Marg ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
