RE: [Histonet] Jores, Klotz and that pesky chloral hydrate
Hi Molly, You might just want to fix in buffered 10% formalin and then rinse specimens well in water ( maybe 20 minutes) before using in class and then put back in formalin. Tom T -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molly Murphy Sent: Thursday, November 20, 2014 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate Hi All, I am looking for a tissue fixative to preserve gross specimens for a veterinary pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's in the past, and Klotz has been recommended, but the chloral hydrate is a problem child due to its status as a controlled substance (eg disposal is a hassle). Does anyone have any thoughts about just leaving the chloral hydrate out? Or, an alternative fixative that doesn't have the chloral hydrate? I have access to a refrigerator for samples, and would *hopefully* keep the specimens for a year or two. Thanks a bunch, M -- Molly Murphy DVM, Ph.D Assistant Professor of Veterinary Pathology College of Natural Sciences Mathematics University of Alaska Fairbanks Office: (907) 474-1990 Fax: (907) 474-1932 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Jores, Klotz and that pesky chloral hydrate
How about formalin substitute from Anatech - Propar I think it is.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: Thursday, November 20, 2014 4:24 PM To: Molly Murphy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Jores, Klotz and that pesky chloral hydrate Hi Molly, You might just want to fix in buffered 10% formalin and then rinse specimens well in water ( maybe 20 minutes) before using in class and then put back in formalin. Tom T -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molly Murphy Sent: Thursday, November 20, 2014 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate Hi All, I am looking for a tissue fixative to preserve gross specimens for a veterinary pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's in the past, and Klotz has been recommended, but the chloral hydrate is a problem child due to its status as a controlled substance (eg disposal is a hassle). Does anyone have any thoughts about just leaving the chloral hydrate out? Or, an alternative fixative that doesn't have the chloral hydrate? I have access to a refrigerator for samples, and would *hopefully* keep the specimens for a year or two. Thanks a bunch, M -- Molly Murphy DVM, Ph.D Assistant Professor of Veterinary Pathology College of Natural Sciences Mathematics University of Alaska Fairbanks Office: (907) 474-1990 Fax: (907) 474-1932 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Jores, Klotz and that pesky chloral hydrate
We are a veterinary pathology teaching lab and we used Klotz with chloral hydrate to preserve our gross teaching specimens. We switched to a Klotz recipe that does not use chloral hydrate because the chloral hydrate is hard to source and very expensive. It is maybe not quite as good as the Klotz with chloral hydrate but seems to work OK. Here is the recipe. Sodium chloride 208 g Sodium bicarbonate 375 g Sodium sulfate 458 g Formaldehyde, 37% 667 ml Water To make up to 50 L Regards, Betty Pollock Manager, Operations DSU Faculty of Veterinary Medicine University of Calgary Calgary, AB, Canada Tel: 403-220-2806 FAX: 403-239-6984 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molly Murphy Sent: Thursday, November 20, 2014 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate Hi All, I am looking for a tissue fixative to preserve gross specimens for a veterinary pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's in the past, and Klotz has been recommended, but the chloral hydrate is a problem child due to its status as a controlled substance (eg disposal is a hassle). Does anyone have any thoughts about just leaving the chloral hydrate out? Or, an alternative fixative that doesn't have the chloral hydrate? I have access to a refrigerator for samples, and would *hopefully* keep the specimens for a year or two. Thanks a bunch, M -- Molly Murphy DVM, Ph.D Assistant Professor of Veterinary Pathology College of Natural Sciences Mathematics University of Alaska Fairbanks Office: (907) 474-1990 Fax: (907) 474-1932 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Jores, Klotz and that pesky chloral hydrate
Well if the specimens are well fixed in formalin, then bring back the colour with alcohol and you can the store them in a non-formalin solution such as dilute honey or high salt solution: Henwood, A., (2002) Color preservation in pathology museum specimens Biotechnic Histochem 77(4):230. Özkan, N., Salva, E., Cakalagaoglu, F., Tüzüner, B. (2012). Honey as a substitute for formalin?. Biotechnic Histochemistry, 87(2), 148-153. Al-Maaini R, Bryant P (2006) The effectiveness of honey as a substitute for formalin in the histological fixation of tissue. J. Histotechnol. 29: 173–176. Oliveira, F. S. (2014). Assessing the effectiveness of 30% sodium chloride aqueous solution for the preservation of fixed anatomical specimens: a 5‐year follow‐up study. J. Anat. (2014) 225, pp118-121 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molly Murphy Sent: Friday, 21 November 2014 8:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate Hi All, I am looking for a tissue fixative to preserve gross specimens for a veterinary pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's in the past, and Klotz has been recommended, but the chloral hydrate is a problem child due to its status as a controlled substance (eg disposal is a hassle). Does anyone have any thoughts about just leaving the chloral hydrate out? Or, an alternative fixative that doesn't have the chloral hydrate? I have access to a refrigerator for samples, and would *hopefully* keep the specimens for a year or two. Thanks a bunch, M -- Molly Murphy DVM, Ph.D Assistant Professor of Veterinary Pathology College of Natural Sciences Mathematics University of Alaska Fairbanks Office: (907) 474-1990 Fax: (907) 474-1932 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
