Re: [Histonet] MMA or Epoxy Embedding

[Michael Gudo (Morphisto GmbH) via Histonet]

Dear Jessica,

the best way to embed screws or any other implant material or bones or teeth or 
any compounds is to Technovit 7200. It a single component Acrylate that hardens 
in blue and white light within a few hours.

We are the exclusive distributor for all Histology Technovits from Kulzer, so 
please contact me personally for further details regarding price, shipping and 
detailed instructions. We can ship worldwide and have some 

Kind regards
Michael

P.S. Here is a short description:
https://www.morphisto.de/en/resin-histology/technovit-resins/technovit-7200/



> Am 21.06.2018 um 20:59 schrieb Jessica Riggleman via Histonet 
> :
> 
> Hi Everyone,
> I am trying to embed screws in plastic (in the hopes to see breakage in the 
> screw, etc). Right now I use specifically a mixture of methyl methacrylate, 
> poly methyl methacrylate, and benzoyl peroxide. However this usually takes a 
> few weeks to embed/dry. I am looking for something a little faster (perhaps a 
> week max?).
> 
> Thank You,
> Jessica
> 
> 
> _
> 
> Jessica Riggleman | Research Associate
> 
> Globus Medical, Inc.
> Valley Forge Business Center
> 2560 General Armistead Avenue | Audubon, PA 19403
> Ph: (610) 930-1800 ext. 2583 | Fax:
> 
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MORPHISTO GmbH
PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
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RE: [Histonet] MMA

[Jack Ratliff]

Reuel,
 
I would say that you could at least try to use for EM, especially since you 
already seem to have a polymerized resin block. The biggest issue I would guess 
would be the overall hardness of your resin block/section so that the section 
would hold up during the analysis. Also, depending upon the hardness of your 
block, you may even be able to put your blocks in an oven at 60C for a weekend 
or so to harden them up even more. It theorized that even though the hardness 
or softness of a MMA block can be established from the beginning of 
polymerization (solution concentration of monomer:softner), the block continues 
to harden over time. I would say subjecting to heat may help to increase this 
however it may make it more difficult to cut thin sections. In fact, if you let 
your section roll during microtomy, you may need to adapt to pulling sections 
flat as the block continues to harden. Hope this makes some sense. Feel free to 
call me (317-281-1975) if you would like to talk about it over the phone.
 
Jack
 

 

 Date: Thu, 18 Aug 2011 14:06:15 -0500
 From: reuel.corne...@tsrh.org
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] MMA
 
 Can undecalcified bone MMA embedded tissue be use for Electron Microscopy.The 
 tissue was fixed in formalin dehydrated in grades of alcohol, clear in 
 xylene, infiltrated and embedded in MMA (MMA, dibutyl phthalate,perakdox). If 
 not,can anyone have a procedure how to prepare tissue that are embedded in 
 MMA for EM. Is this the same procedure done on a paraffin embedded tissue 
 where you melt the paraffin with xylene then hydrate, wash in distilled water 
 then transfer in osmium ,wash in water, dehydrate, PO,resin. 
 
 Thank you for your help.
 
 Reuel
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Re: [Histonet] MMA Formulation for EXAKT Grinding

[Jack Ratliff]

Nikki,

The MMA formulation you describe is one that yields a very soft block suitable 
for thin section (4-6 microns) microtomy. I have heard where people use this 
formulation for undemineralized bone and maybe even for stent work, but the 
softness of this formulation concerns me for the stents given the reduced 
stability. For me personally it is not what I use for both of these specimen 
types, but that is for another discussion.

It is important to note that the volume of catalyst is proportional to the 
total volume of solution and thus this ratio (expressed as w/w) is directly 
proportional to the reaction product. Now to make things a little more 
confusing, the reaction product is influenced by air temperature, the 
size/density of the specimen, the total volume of reaction product, and 
sometimes the embedding container and void space above the solution level and 
container lid. Furthermore, since this reaction or polymerization of resin is 
an exothermic reaction, the rate (expressed as a unit of time) at which the 
reaction reaches the actual point at which polymerization initiates (v-max) 
also then influences the amount of heat that is generated from the reaction. 
This then is proportional to the quality of polymerization that can be seen as 
either a hard clear desirable block or and over polymerized, bubbled mess!!!

It is my opinion that the bubbles in your specimen blocks are related to the 
build up of pressure in your container and caused by a rapid polymerization of 
your specimens by the use of the heated water bath (as per you concentration of 
catalyst to MMA/DBP solution) and lack of void space to buffer or diffuse 
excess heat. My feeling is that you are using too much catalyst in conjunction 
with the heat of the water bath to polymerize these specimens. Also, what is 
the volume of the solution you are polymerizing, how close are your specimen 
molds to each other in the water bath, and is the water level of the water bath 
at or above the embedding solution level in the specimen container? The heat 
generated from one specimen can sometimes add to the heat generated by another 
in close proximity. This then results sometimes in an over polymerization of 
one specimen (too much heat generated in the reaction) and no polymerization of 
another (absence of heat to drive the reaction).

Here are my suggestions:

1) If you need specimens polymerized immediately the next day, take care to 
space out your specimens further apart in the water bath. Also, try turning 
down the water bath to reduce the secondary heat used to drive the reaction. If 
none of this works, then look at reducing the amount of catalyst used (may want 
to do this first and keep everything else the same).

2) If you can spare a few days, don't change a thing with the embedding 
solution, try switching your molds to polypropylene containers and leave them 
out on the counter at room temperature (22-23C) for 2-3 days until they 
polymerize.

Hope this helps and it wasn't too confusing.

Jack


On Jun 25, 2010, at 3:46 PM, Wahlberg, Nikki nikki.wahlb...@bsci.com wrote:

 Hello Everyone,
 
 I was wondering if you could help me with my MMA formulation.  I have been 
 using a formulation that I found in a published paper.  My current embedding 
 formulation is 80ml MMA, 20ml Dibutyl Phthalate and 3g Benzoyl Peroxide.  The 
 samples are embedded after three days of infiltration, one change per day, 
 with the formulation of 80ml MMA and 20ml Dibutyl Phthalate. Lately have 
 noticed that there is a pressure build up in the vials.  I have had a few 
 vials burst almost immediately once placed in the heated waterbath. I am 
 filling the glass vials full with the embedding solution, capping them and 
 then placing them in a water bath in a 37 degree oven.  They are completely 
 polymerized by the next morning. I am also getting bubbles in the plastic 
 when polymerized.
 
 I have two questions: Is there any way to get rid of the bubbles in the 
 plastic and of more concern what do you think is causing the pressure build 
 up?
 
 I would really appreciate any help that you can provide.
 
 Thank you,
 Nikki
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Re: [Histonet] MMA Sections

[Jack Ratliff]

Karen,

How about coating your slides with Haupt's. I have used this solution  
exclusively for all my undemineralized thin section bone work. Also, I  
only need to dry my slides overnight using a dense aluminum slide  
press (available via Dorn and Hart Microedge). Basically, the slide  
press acts to evenly flatten and press the sections to the Haupt's  
coated slide and also evenly heat all the slides together. Because of  
it's heat conducting properties, the press acts as a mini-oven within  
the oven to speed up the drying and section adhesion process. Please  
feel free to contact me if you have any questions regarding this or  
any other resin related topic.



Regards,

Jack Ratliff
NSH Hard Tissue Committee Chair


On Dec 1, 2009, at 9:52 AM, Karen Hughes hugh...@upstate.edu wrote:

I am currently embeding 2cm sections of adult rat spine in MMA  
including
muscle, bone and spinal cord.  I then cut 5um sections using a  
tungsten
carbide D profile knife on a manual microtome and mount the sections  
on APES
coated slides, press them in a vise between tongue depressors, and  
bake them
@ 41'C for a min. of 48hrs.  My problem is that when I de-placticize  
the
slides in Xylene (4 changes, 2x5min, 2x10min) the spinal cord falls  
off the

slide, while the bone stays secure.
I have also tried PolyLysine coated slides, SuperFrost Plus, and  
Probe On

Slides.  All with the same results.

Does anyone have any experience with this?  Any and all Suggestions  
would be

greatly appreciated.

Karen

Karen S. Hughes
Research Support Specialist
SUNY Upstate Medical University
Phone (315)464-8585
hugh...@upstate.edu

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RE: [Histonet] MMA Dehydration

[Wahlberg, Nikki]

We are mainly processing vessels that can range in size of 10mm-200mm in
length.  The different types of vessels are either peripheral, cardiac
or pulmonary trunks.  
 
Nikki



From: Jack Ratliff [mailto:ratliffj...@hotmail.com] 
Sent: Tuesday, June 02, 2009 1:27 PM
To: Wahlberg, Nikki
Subject: RE: [Histonet] MMA Dehydration


What is the tissue you are wanting to process???
 
Jack Ratliff
 

 
 Date: Tue, 2 Jun 2009 13:19:49 -0500
 From: nikki.wahlb...@bsci.com
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] MMA Dehydration
 
 Hello Everyone,
 
 I am wondering if anyone would be willing to share their MMA
dehydration
 schedules? We are currently trying to improve our EXAKT MMA specimens.
 We currently process with the following schedule:
 10% Formalin 4 hours
 70% Ethyl Alcohol 1 hour
 95% Ethyl Alcohol 2 hours
 95% Ethyl Alcohol 2 hours
 100% Ethyl Alcohol 3 hours
 100% Ethyl Alcohol 3 hours
 
 Thank you,
 Nikki
 
 Nikki M Wahlberg
 Histotechnologist II
 Histo-Pathology Services
 Boston Scientific
 763-694-5739
 wahlb...@bsci.com
 
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