Surgical Pathology Technologist
Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General
Hospital | An Affiliate of Commonwealth Health |
mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre,
PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526
-Original Message-
From: Barr,Kaye H [mailto:khb...@mdanderson.org]
Sent: Tuesday, April 30, 2013 1:49 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Histonet Digest, Vol 113, Issue 30
What title do most use for someone who does gross and is not a PA?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Tuesday, April 30, 2013 12:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 113, Issue 30
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Re: Contents of Histonet digest...
Today's Topics:
1. BIG PROBLEM (Behnaz Sohrab)
2. Re: BIG PROBLEM (Linda)
3. Yearly Training (Nancy Schmitt)
4. HIER pressure cookers (k. leigh adams)
5. RE: HIER pressure cookers (Connolly, Brett M)
6. RE: HIER pressure cookers (Blazek, Linda)
7. Re: HIER pressure cookers (Jan Shivers)
8. RE: 5 gal specimen container search (Hannen, Valerie)
9. RE: 5 gal specimen container search (Shirley A. Powell)
10. RE: HIER pressure cookers (dingers...@aplaboratories.com)
11. AW: [Histonet] BIG PROBLEM (Gudrun Lang)
12. Processor question - alcoholic formalin vs. buffered formalin
(Lake, Kim S)
13. RE: Processor question - alcoholic formalin vs. buffered
formalin (Hannen, Valerie)
14. cassette labelers (Margiotta-Watz, Michele)
--
Message: 1
Date: Mon, 29 Apr 2013 10:36:01 -0700
From: Behnaz Sohrab sohra...@ah.org
Subject: [Histonet] BIG PROBLEM
To: histonet@lists.utsouthwestern.edu
Message-ID: 517e4d11.4347.005...@ah.org
Content-Type: text/plain; charset=us-ascii
Good Morning, We have two Leica HE stainer( old xt-5010 and new 5020).
We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds
Bluing : 45 sec. We done this for years!! last few months our
Pantologists are complaining about GI Biopsies that they have TOO much
blue in cytoplasma and the Mucin is too purple and blue? Please any
suggestion??
Mean while we are trying the NEW kit from Leica for HE , they think
that is TOO pale?any of you has tried this new stain from Lecia?
With much application.
Behnaz Sohrab, Los Angeles
--
Message: 2
Date: Mon, 29 Apr 2013 11:30:38 -0700 (PDT)
From: Linda lmd...@yahoo.com
Subject: Re: [Histonet] BIG PROBLEM
To: Behnaz Sohrab sohra...@ah.org,
histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:
1367260238.82176.yahoomail...@web164002.mail.gq1.yahoo.com
Content-Type: text/plain; charset=iso-8859-1
Hi,
?
I would first check with your alcohol and xylene suppliers to see if
they changed anything.? When something?goes south it is usually because
the manufacturer have changed.? This happened with my silver stains.
?
You may have to go back to the basics- harris hematoxylin, acid alcohol,
and eosin y.? Richard Allen 7211 hematoxylin and the rest of their
staining is excellent in these situations until you can get it back
under control.? If you send them slides they will work a time out for
you.
?
If you are using Leica's selectech, which is what I started this lab out
with because I was heavily sampled out,?it has blue nuclei instead of
the traditional purple.? I do 5 minutes in hematoxylin, 30 dips in
clarifier and 1 minute in blue, 55 sec in eosin but I have (2) 95s
after.? They also have a tech dept to help.? I have a Leica autostainer
too.
?
I hope this helps.
?
Linda Dee, HT(ASCP)
?
From: Behnaz Sohrab sohra...@ah.org
To: histonet@lists.utsouthwestern.edu
Sent: Monday, April 29, 2013 12:36 PM
Subject: [Histonet] BIG PROBLEM
Good Morning, We have two Leica HE stainer( old xt-5010 and new 5020).
We do use : Gill's 3 for 6 minutes,
4% Glacial ACETIC Acid 40 seconds
Bluing : 45 sec. We done this for years!! last few months our
Pantologists are complaining about GI Biopsies that they have TOO much
blue in cytoplasma and the Mucin is too purple and blue? Please any
suggestion??
Mean while we are trying the NEW kit from Leica for HE , they think
that is TOO pale?any of you has tried this
