Re: [Histonet] Removing tissues from OCT for molecular analysis

2010-03-29 Thread Malika Benatti 
What you should do ideally is sample you tissue for molecular studie  
first, then carry out frozen sections


Malika


  ... Smile it confuses people ...

On 29 Mar 2010, at 15:31, Katie Crosby kcro...@cellsignal.com wrote:

I would like to remove tissues from blocks of OCT and perform  
molecule studies.  I need to separate tissues from OCT.

Thanks,
Katie

On Mar 29, 2010, at 10:21 AM, Malika Benatti wrote:


Do you mean removing OCT from tissue ?

 ... Smile it confuses people ...

On 29 Mar 2010, at 13:55, Katie Crosby kcro...@cellsignal.com  
wrote:



Hello,

Can someone suggest a means of removing tissues from OCT for  
subsequent molecular analysis?  I did not find anything in the  
archives describing this exactly.


Thanks,

Katie






Katie Crosby
Immunohistochemistry
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923

Phone: 978-867-2352
Fax:  978-867-2400
WEB Site:   www.cellsignal.com

Toll Free:
1-877-616-CELL  (877-616-2355)
1-877-678-TECH  (877-678-8324)




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Katie Crosby
Immunohistochemistry
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923

Phone: 978-867-2352
Fax:  978-867-2400
WEB Site:   www.cellsignal.com

Toll Free:
1-877-616-CELL  (877-616-2355)
1-877-678-TECH  (877-678-8324)





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RE: [Histonet] Removing tissues from OCT for molecular analysis

2010-03-29 Thread Tony Henwood 
Katie,

Rinse the frozen OCT surrounded tissue in Hanks or similar cell culture
fluid. Two to three times should be enough to remove the OCT

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager  Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Katie
Crosby
Sent: Monday, 29 March 2010 11:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Removing tissues from OCT for molecular analysis


Hello,

Can someone suggest a means of removing tissues from OCT for  
subsequent molecular analysis?  I did not find anything in the  
archives describing this exactly.

Thanks,

Katie






Katie Crosby
Immunohistochemistry
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923

Phone: 978-867-2352
Fax:  978-867-2400
WEB Site:   www.cellsignal.com

Toll Free:
1-877-616-CELL  (877-616-2355)
1-877-678-TECH  (877-678-8324)




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