Re: [Histonet] Von Kossa Stain for calcium
We are a research lab and don't do a big volume of special stains. We just received a project for 35 slides that need Von Kossa Stain. I acid-washed some small glass staining dishes-no rack required (in the past, we only had a few requests). Can I run slides in plastic slide holders (24-place) in an acid-washed beaker for the silver nitrate step? Any tips would be appreciated. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Von Kossa stain kit
Thought of another question! What other vendors do people order special stain kits? We have been using Poly Scientific, which is very good, but costly. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Von Kossa stain kit
It has been my experience that you do not need to acid wash the glassware for the silver nitrate step. The only critical parts are the time (5 minutes) in silver nitrate (protected from light), the 3 dH2O rinses (also protected from light), developing time (2 minutes) in sodium carbonate-formaldehyde solution (protected from light), 2 dH2O rinses (no need to protect from light), the 30 seconds in Farmer's Diminisher (sodium thiosulfate-potassium ferricyanide solution) and tap water rinse. The sodium-carbonate formaldehyde solution is use to chemically develop the silver attachment to any presence of mineralization. Most people use natural light, but the chemical step is more consistent in my opinion. Additionally, since the silver nitrate step is used to attach silver ions to the presence of minerals, the use of any metal is prohibited as it will also bind silver. However, if you use plastic, glass, or even stainless steel, no worries at all. As for a reliable kit, I use the kit from Dorn and Hart Microedge (www.dornandhart.com). You can also choose you preferred counterstain with this kit. I routinely work with mineralized bone and therefore use the Von Kossa - MacNeal's Tetrachrome stain kit. Hope this helps! Best, Jack Date: Tue, 4 Oct 2011 11:02:45 -0400 From: msherw...@partners.org To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Von Kossa stain kit Thought of another question! What other vendors do people order special stain kits? We have been using Poly Scientific, which is very good, but costly. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: Re: [Histonet] Von Kossa stain kit
I've used Rowley Biochemical for years: http://rowleybio.com/ Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Tuesday, October 04, 2011 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Von Kossa stain kit Thought of another question! What other vendors do people order special stain kits? We have been using Poly Scientific, which is very good, but costly. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Von kossa stain
Funny you should ask... just last week I had two requests for a Von Kossa. That's when I found out that, despite the fact that we just moved into a brand new building with all the bells and whistles, there was not one single UV light in any of the many hoods that had been installed. Heaven forbid I could even find an incandescent bulb (we are a green building). So, I prepped my slides, put them in a clear glass Coplin jar and parked the jar on the hood of my car for an hour. Works like a charm. This all depends, naturally, on (1) if you even HAVE sunshine where you live; (2) how far a walk it is to the sunshine, and (3) whether you have a car hood on which to park the Coplin jar. Needless to say, out of the line of sight of curious onlookers with sticky fingers and no business wondering what that glass jar is... Nonetheless, it works just fine! May the Force be with you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Von kossa stain
You can use any strong intensity microscope light. The stronger the intensity of the light the better, but the reduction has a cumulative effect so even not very strong intensity bulbs can be effective. René J. --- On Tue, 4/12/11, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: From: Webb, Dorothy L dorothy.l.w...@healthpartners.com Subject: [Histonet] Von kossa stain To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Date: Tuesday, April 12, 2011, 1:35 PM What is everyone using for their light when developing the silver in the VonKossa stain when you have no sunlight to use? We used to use a 60 watt lamp, but haven't done one for years and am bringing this stain back to our repetiore due to pathologist request. Thanks much! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Von kossa stain
I purchase a kit from Dorn and Hart Microedge and use sodium carbonate-formaldehyde solution to chemically develop. Stain in silver nitrate for 5 min in the dark, 3 fresh DI rinses for 1 min each in the dark, then sodium carbonate-formaldehyde solution for 2 min in the dark, 2 fresh DI rinses (normal light) for 1 min each, 30 seconds in Farmer's Diminisher (Sodium thiosulfate-potassium ferricyanide soluiton to stop reaction) and a running tap water rinse for 10 min. Jack From: dorothy.l.w...@healthpartners.com To: histonet@lists.utsouthwestern.edu Date: Tue, 12 Apr 2011 12:35:18 -0500 Subject: [Histonet] Von kossa stain What is everyone using for their light when developing the silver in the VonKossa stain when you have no sunlight to use? We used to use a 60 watt lamp, but haven't done one for years and am bringing this stain back to our repetiore due to pathologist request. Thanks much! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Von kossa stain
Interesting, Meloan Puchtler (1985) have drawn our attention to Von Kossa's original work on this technique. He regarded only the yellow colouration of calcium deposits during early stages of the reaction as diagnostic for calcium phosphate and credited the blackening to organic matter. Further studies showed that bright light only causes the irreversible blackening of organic matter that masks the yellow silver phosphate. When the reaction is performed in subdued light, yellow to yellowish brown silver phosphate is visualised selectively. Silver carbonate dissolves in sodium thiosulphate and cannot be demonstrated with von Kossa's technique (Meloan Puchtler 1985 J Histotechnol 8(1):11-13.). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, 13 April 2011 3:53 AM To: Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Von kossa stain Funny you should ask... just last week I had two requests for a Von Kossa. That's when I found out that, despite the fact that we just moved into a brand new building with all the bells and whistles, there was not one single UV light in any of the many hoods that had been installed. Heaven forbid I could even find an incandescent bulb (we are a green building). So, I prepped my slides, put them in a clear glass Coplin jar and parked the jar on the hood of my car for an hour. Works like a charm. This all depends, naturally, on (1) if you even HAVE sunshine where you live; (2) how far a walk it is to the sunshine, and (3) whether you have a car hood on which to park the Coplin jar. Needless to say, out of the line of sight of curious onlookers with sticky fingers and no business wondering what that glass jar is... Nonetheless, it works just fine! May the Force be with you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
