RE: SPAM-LOW: RE: [Histonet] Water collecting at bottom of sections
I always tap the slide on the counter before standing it up to drain after picking it up off the water bath, I think this should shake loose the bag of water you are talking about collecting under some sections. I actually hit the non label end of the slide on the counter pretty hard, I have had people ask me if I ever brake the slide, but I have not ever broken a slide by doing this. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Collette, Nicole M. Sent: Friday, July 23, 2010 2:06 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] Water collecting at bottom of sections I use FisherBrand SuperFrost Plus charged slides, the only time I have this problem is when my waterbath is too hot, or if for some reason I use warmed slides to retrieve my sections from the bath (like if you lay the unused slides on the edge of the waterbath, or if you use the warm droplet method to spread your sections)? I haven't had much experience with a lot of different slide types/brands though, but it might be an easy fix to the problem you hadn't thought about, as it is more or less unrelated to the brand of slide, although some brands may be more sensitive to this issue... Sincerely, Nicole Collette Lawrence Livermore National Lab/ UC Berkeley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of nap...@mail.siscom.net Sent: Friday, July 23, 2010 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water collecting at bottom of sections Hello all, From time to time and depending on what brand of adhesive (or charged) slides I am using, I seem to get a bag of water that drains to the bottom of my sections but doesn't drain out. I have been working in microtomy a long time and have had to deal with this contingency time and time again, but never really have gotten to the bottom of the problem. I spoke with a premium manufacturer of such slides and they seemed to indicate that it is a problem with the coating, but couldn't tell me for sure. All I know is that certain brands do this more than others. If you know what I mean, you know it is a problem. My bath is pure distilled H2O with no gelatin or Sta-on added. It is if the adhesive properties are SO good that they will not release the water when vertically drained and have to be shaken off or cut with a razor blade at bottom to release the water. Anyway, if anyone has an insight or two on this, I would be interested. It seem sthe most challenging issues are ones that seem related to some of the most simple tasks that one has performed for many years!! Manufacturers understand what I mean, but cannot pinpoint the problem for me via phone or e-mail. Anyone see this and have a chemical/mechanical solution they have developed over the years? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://*lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Water collecting at bottom of sections
I routinely flick my wrist holding the slide with the section I've just picked up. Usually, this is enough force to release any water at the bottom of the section. If that doesn't work I melt a tiny hole at the bottom of the section with a heated probe and flick again. I'm sure the manufacturer is right in saying that its the coating; the paraffin adheres too well and too quickly to the slide trapping water underneath. It is also very important to hold your slides as vertically as possible when bringing them under your section and raising the slide out of the water bath. That way you will trap as little water as possible underneath. Hope this helps. From one long-time-microtomist to another, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of nap...@mail.siscom.net Sent: Friday, July 23, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water collecting at bottom of sections Hello all, From time to time and depending on what brand of adhesive (or charged) slides I am using, I seem to get a bag of water that drains to the bottom of my sections but doesn't drain out. I have been working in microtomy a long time and have had to deal with this contingency time and time again, but never really have gotten to the bottom of the problem. I spoke with a premium manufacturer of such slides and they seemed to indicate that it is a problem with the coating, but couldn't tell me for sure. All I know is that certain brands do this more than others. If you know what I mean, you know it is a problem. My bath is pure distilled H2O with no gelatin or Sta-on added. It is if the adhesive properties are SO good that they will not release the water when vertically drained and have to be shaken off or cut with a razor blade at bottom to release the water. Anyway, if anyone has an insight or two on this, I would be interested. It seem sthe most challenging issues are ones that seem related to some of the most simple tasks that one has performed for many years!! Manufacturers understand what I mean, but cannot pinpoint the problem for me via phone or e-mail. Anyone see this and have a chemical/mechanical solution they have developed over the years? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Water collecting at bottom of sections
I experienced it almost daily and thought this was normal!? I just dipped the slides gently on a paper towel right after getting them on the slide and shaking, for the case I could not just shake it off. Indeed, as I remember, the Super Frost Plus slides had to be dipped a bit harder to let the water rinse off. Hello all, From time to time and depending on what brand of adhesive (or charged) slides I am using, I seem to get a bag of water that drains to the bottom of my sections but doesn't drain out. I have been working in microtomy a long time and have had to deal with this contingency time and time again, but never really have gotten to the bottom of the problem. I spoke with a premium manufacturer of such slides and they seemed to indicate that it is a problem with the coating, but couldn't tell me for sure. All I know is that certain brands do this more than others. If you know what I mean, you know it is a problem. My bath is pure distilled H2O with no gelatin or Sta-on added. It is if the adhesive properties are SO good that they will not release the water when vertically drained and have to be shaken off or cut with a razor blade at bottom to release the water. Anyway, if anyone has an insight or two on this, I would be interested. It seem sthe most challenging issues are ones that seem related to some of the most simple tasks that one has performed for many years!! Manufacturers understand what I mean, but cannot pinpoint the problem for me via phone or e-mail. Anyone see this and have a chemical/mechanical solution they have developed over the years? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Water collecting at bottom of sections
I second Linda's technique, the only other option I use is to bring the slide up at a negative angle so the slide comes out of the water bath first then the section. I find this works well for us. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Friday, July 23, 2010 2:42 PM To: nap...@mail.siscom.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Water collecting at bottom of sections I routinely flick my wrist holding the slide with the section I've just picked up. Usually, this is enough force to release any water at the bottom of the section. If that doesn't work I melt a tiny hole at the bottom of the section with a heated probe and flick again. I'm sure the manufacturer is right in saying that its the coating; the paraffin adheres too well and too quickly to the slide trapping water underneath. It is also very important to hold your slides as vertically as possible when bringing them under your section and raising the slide out of the water bath. That way you will trap as little water as possible underneath. Hope this helps. From one long-time-microtomist to another, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of nap...@mail.siscom.net Sent: Friday, July 23, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water collecting at bottom of sections Hello all, From time to time and depending on what brand of adhesive (or charged) slides I am using, I seem to get a bag of water that drains to the bottom of my sections but doesn't drain out. I have been working in microtomy a long time and have had to deal with this contingency time and time again, but never really have gotten to the bottom of the problem. I spoke with a premium manufacturer of such slides and they seemed to indicate that it is a problem with the coating, but couldn't tell me for sure. All I know is that certain brands do this more than others. If you know what I mean, you know it is a problem. My bath is pure distilled H2O with no gelatin or Sta-on added. It is if the adhesive properties are SO good that they will not release the water when vertically drained and have to be shaken off or cut with a razor blade at bottom to release the water. Anyway, if anyone has an insight or two on this, I would be interested. It seem sthe most challenging issues are ones that seem related to some of the most simple tasks that one has performed for many years!! Manufacturers understand what I mean, but cannot pinpoint the problem for me via phone or e-mail. Anyone see this and have a chemical/mechanical solution they have developed over the years? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Water collecting at bottom of sections
I use FisherBrand SuperFrost Plus charged slides, the only time I have this problem is when my waterbath is too hot, or if for some reason I use warmed slides to retrieve my sections from the bath (like if you lay the unused slides on the edge of the waterbath, or if you use the warm droplet method to spread your sections)? I haven't had much experience with a lot of different slide types/brands though, but it might be an easy fix to the problem you hadn't thought about, as it is more or less unrelated to the brand of slide, although some brands may be more sensitive to this issue... Sincerely, Nicole Collette Lawrence Livermore National Lab/ UC Berkeley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of nap...@mail.siscom.net Sent: Friday, July 23, 2010 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water collecting at bottom of sections Hello all, From time to time and depending on what brand of adhesive (or charged) slides I am using, I seem to get a bag of water that drains to the bottom of my sections but doesn't drain out. I have been working in microtomy a long time and have had to deal with this contingency time and time again, but never really have gotten to the bottom of the problem. I spoke with a premium manufacturer of such slides and they seemed to indicate that it is a problem with the coating, but couldn't tell me for sure. All I know is that certain brands do this more than others. If you know what I mean, you know it is a problem. My bath is pure distilled H2O with no gelatin or Sta-on added. It is if the adhesive properties are SO good that they will not release the water when vertically drained and have to be shaken off or cut with a razor blade at bottom to release the water. Anyway, if anyone has an insight or two on this, I would be interested. It seem sthe most challenging issues are ones that seem related to some of the most simple tasks that one has performed for many years!! Manufacturers understand what I mean, but cannot pinpoint the problem for me via phone or e-mail. Anyone see this and have a chemical/mechanical solution they have developed over the years? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://*lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Water collecting at bottom of sections
Am I the only person who paraffin sections by: cutting the sections, grabbing the ribbon with forceps or paintbrush, placing it on a dry slide, adding water underneath the ribbon with a pastuer pipet, and then placing the slide on a slide warmer. The water will heat up and the paraffin ribbon will expand on the bubble of water on the slide.Leave the slide on a slide warmer overnight and the water is evaporates. Some people blot off the extra water. Maybe that's just not any easier than picking up the sections from a water bath. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
