Re: [Histonet] Blades
In China we can buy Leica, Feather, or domestic. The domestic ones are not uniformly good or bad. I am really really tired of Feather and I consider that my time is worth something so I insist that we use Leica although they are as "high as a cat's back". > ---Original Message--- > From: raestask via Histonet > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Blades > Sent: Nov 18 '20 02:38 > > What breand of blades do people prefer??Rae Staskiewicz UnityPoint Health > Methodist PeoriaSent from my Verizon, Samsung Galaxy smartphone > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] blades
Hello does anyone have a source for Personna Single-edge stainless steel razor blades? Our former vendor no longer stocks them. Thanks, Atoska ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] blades
Lauren Sweeney at the University of Georgia asks: >>Blades for grossing tissues- anyone have recommendations? The vendor that we were purchasing just straight razor blades from has stopped manufacturing them. We don't use scalpels because of their higher cost and we are only working with poultry samples.<< I just Googled "single edge razor blades" and they're still available from a number of sources. I've done hospital pathology grossing with them, though not in the last 40 years, and found them extremely satisfactory - in fact, I prefer them to scalpel blades. I've been shaving my face since 1951, and have never heard of anybody actually using them to shave with. Bob Richmond gray bearded samurai pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Blades for cutting resin on a microtome
Veronique, May I ask what type of specimen is embedded into the JB-4 resin? Nevertheless, you should be able to cut these blocks using a tungsten-carbide knife. While there are a few vendors out there that sell these knives, in my laboratory I personally use knives re-sharpened by Delaware Diamond Knives (DDK). Please feel free to message me privately if you need further assistance as I have been working with resin embedded specimens for over 17 years. I will also encourage you to reach out to Sarah Mack as she is the new Hard Tissue Committee Chairperson for the National Society for Histotechnology. You can find her contact information and additional information about the committee by visiting www.nsh.org! Best Regards, Jack Ratliff On Sep 12, 2014, at 9:32 AM, Véronique Barrès veronique.bar...@gmail.com wrote: Happy Friday Histonetters! I am working on a histology platform in a research center and someone came to me last week and asked to cut blocs of resin (JB-4 resin) on the microtome. I never cut anything else than paraffin, so I was wondering if some of you had advices for me? They never did it neither and took their protocol in a paper where it was said that we should use disposable glass knife instead of standard metal blades. Are any of you ever used those knife? Where do you buy them? We have an old Leica RM2125. Thanks for your advices! Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Blades for cutting resin on a microtome
I've only cut resin with a glass or diamond knife in an ultramicrotome. If you are attempting to do it in a regular microtome, you would need a special blade holder. I don't know if any microtome manufactures make glass knife holders. You make the glass blades yourself using special glass. Here is a link to the glass strips: http://www.sigmaaldrich.com/catalog/product/sigma/g2528?lang=enregion=US Here is a cheap jig and diamond glass cutters it make the knifes: https://www.emsdiasum.com/microscopy/products/preparation/glassknife.aspx I've never made glass knives by hand using a hand held diamond cutter and jigs. I imagine that it would take practice. I've only used a specialized maker: https://www.emsdiasum.com/microscopy/products/histology/tissue_stainer.aspx You paint a bit of nail polish underneath the glass edge and put a bit of distilled water on the edge. You then section the block floating the sections on the water. Use an eyelash manipulator to pick up the 5um thick sections and place on a bubble of water on the slide. Evaporate the water droplet on the slide. If you've done it right, the sections won't look like origami. If it does, then practice until it doesn't. Gerry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès Sent: Friday, September 12, 2014 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades for cutting resin on a microtome Happy Friday Histonetters! I am working on a histology platform in a research center and someone came to me last week and asked to cut blocs of resin (JB-4 resin) on the microtome. I never cut anything else than paraffin, so I was wondering if some of you had advices for me? They never did it neither and took their protocol in a paper where it was said that we should use disposable glass knife instead of standard metal blades. Are any of you ever used those knife? Where do you buy them? We have an old Leica RM2125. Thanks for your advices! Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Blades for cutting resin on a microtome
Yes brightinstruments, com make glass knife holder and tungsten carbide tipped knives for microtomes, KR,Alan Bright Sent from my iPhone On 12 Sep 2014, at 15:49, Keyser Gerald T gkey...@uwhealth.org wrote: I've only cut resin with a glass or diamond knife in an ultramicrotome. If you are attempting to do it in a regular microtome, you would need a special blade holder. I don't know if any microtome manufactures make glass knife holders. You make the glass blades yourself using special glass. Here is a link to the glass strips: http://www.sigmaaldrich.com/catalog/product/sigma/g2528?lang=enregion=US Here is a cheap jig and diamond glass cutters it make the knifes: https://www.emsdiasum.com/microscopy/products/preparation/glassknife.aspx I've never made glass knives by hand using a hand held diamond cutter and jigs. I imagine that it would take practice. I've only used a specialized maker: https://www.emsdiasum.com/microscopy/products/histology/tissue_stainer.aspx You paint a bit of nail polish underneath the glass edge and put a bit of distilled water on the edge. You then section the block floating the sections on the water. Use an eyelash manipulator to pick up the 5um thick sections and place on a bubble of water on the slide. Evaporate the water droplet on the slide. If you've done it right, the sections won't look like origami. If it does, then practice until it doesn't. Gerry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès Sent: Friday, September 12, 2014 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades for cutting resin on a microtome Happy Friday Histonetters! I am working on a histology platform in a research center and someone came to me last week and asked to cut blocs of resin (JB-4 resin) on the microtome. I never cut anything else than paraffin, so I was wondering if some of you had advices for me? They never did it neither and took their protocol in a paper where it was said that we should use disposable glass knife instead of standard metal blades. Are any of you ever used those knife? Where do you buy them? We have an old Leica RM2125. Thanks for your advices! Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Blades for cutting resin on a microtome
I used to have a triangle glass knife holder insert for my Leica microtome or I would use the tungsten carbide knives. It depends on what you are cutting. if it is calcified bone the glass knives scratch too much and they are only 1/2 inch wide so you have to cut smaller soft tissues with them. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net From: abri...@brightinstruments.com Date: Fri, 12 Sep 2014 17:32:47 +0100 To: gkey...@uwhealth.org Subject: Re: [Histonet] Blades for cutting resin on a microtome CC: histonet@lists.utsouthwestern.edu; veronique.bar...@gmail.com; exp...@brightinstruments.com Yes brightinstruments, com make glass knife holder and tungsten carbide tipped knives for microtomes, KR,Alan Bright Sent from my iPhone On 12 Sep 2014, at 15:49, Keyser Gerald T gkey...@uwhealth.org wrote: I've only cut resin with a glass or diamond knife in an ultramicrotome. If you are attempting to do it in a regular microtome, you would need a special blade holder. I don't know if any microtome manufactures make glass knife holders. You make the glass blades yourself using special glass. Here is a link to the glass strips: http://www.sigmaaldrich.com/catalog/product/sigma/g2528?lang=enregion=US Here is a cheap jig and diamond glass cutters it make the knifes: https://www.emsdiasum.com/microscopy/products/preparation/glassknife.aspx I've never made glass knives by hand using a hand held diamond cutter and jigs. I imagine that it would take practice. I've only used a specialized maker: https://www.emsdiasum.com/microscopy/products/histology/tissue_stainer.aspx You paint a bit of nail polish underneath the glass edge and put a bit of distilled water on the edge. You then section the block floating the sections on the water. Use an eyelash manipulator to pick up the 5um thick sections and place on a bubble of water on the slide. Evaporate the water droplet on the slide. If you've done it right, the sections won't look like origami. If it does, then practice until it doesn't. Gerry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès Sent: Friday, September 12, 2014 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades for cutting resin on a microtome Happy Friday Histonetters! I am working on a histology platform in a research center and someone came to me last week and asked to cut blocs of resin (JB-4 resin) on the microtome. I never cut anything else than paraffin, so I was wondering if some of you had advices for me? They never did it neither and took their protocol in a paper where it was said that we should use disposable glass knife instead of standard metal blades. Are any of you ever used those knife? Where do you buy them? We have an old Leica RM2125. Thanks for your advices! Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Blades
I have been in histology and cutting for about 26 years now. I have used many types of blades, high and low profile. My favorite and I think the best ones are Surgipath Teflon coated high or low profile blades. I believe the high profile blades are the best but I do realize that not everyone has that option. I think if you tried them you wouldn't be disappointed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hale, Meredith Sent: Monday, April 15, 2013 12:39 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades I would like some feedback from you on the types of blades those of you who cut GI biopsies prefer ? Do you see differences with chatter on different blades. Any feedback is appreciated . Thanks ! Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mh...@miracals.commailto:mh...@miracals.commailto:mh...@miracals.com%3 cmailto:mh...@miracals.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] blades
Dorothy, I put ours in a 15 mL centrifuge tube with a cap sit it on the base of the microtome for the next use, that way, no one gets cut the blade is able to be used to the fullest of it's potential. :-) Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbr...@andrew.cmu.edu On Jun 24, 2011, at 4:53 PM, Webb, Dorothy L wrote: Trying to clean up some things hanging out there in our lab and wondering what everyone does with a blade that has been used minimally and tech done for the day with the microtome. Where do you store that blade for use tomorrow or do you toss and not worry about the cost involved? I do not like them sitting on top of the microtome. Any good ideas?? Thanks, as always! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] blades
I use the cardboards that come in a box of slides. A small piece of tape on the open side and mark it used. I just always make sure I have the blade edge facing the folded part. I know some who will tape this folded board it to the side of their microtome and use it as a trimming blade holder. Vikki On Fri, Jun 24, 2011 at 5:10 PM, Esther C Peters epete...@gmu.edu wrote: We put ours in a small slide box (plastic or styrofoam, 5-25 slides) that is clearly marked Microtome Blades for Facing Blocks to be used another day. Esther C. Peters, Ph.D. Assistant Professor Department of Environmental Science Policy Biology Program/Medical Technology Coordinator George Mason University 4400 University Drive, MSN 5F2 Fairfax, VA 22030- Office: David King Hall 3057 Phone: 703-993-3462 Fax: 703-993-1066 epete...@gmu.edu - Original Message - From: Webb, Dorothy L dorothy.l.w...@healthpartners.com Date: Friday, June 24, 2011 4:53 pm Subject: [Histonet] blades Trying to clean up some things hanging out there in our lab and wondering what everyone does with a blade that has been used minimally and tech done for the day with the microtome. Where do you store that blade for use tomorrow or do you toss and not worry about the cost involved? I do not like them sitting on top of the microtome. Any good ideas?? Thanks, as always! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967- 6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] blades
We use plastic 5 slide mailers Sent from my iPhone On Jun 24, 2011, at 1:53 PM, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: Trying to clean up some things hanging out there in our lab and wondering what everyone does with a blade that has been used minimally and tech done for the day with the microtome. Where do you store that blade for use tomorrow or do you toss and not worry about the cost involved? I do not like them sitting on top of the microtome. Any good ideas?? Thanks, as always! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] blades
Jennifer, Ooh, I like that! I'll start doing the same first thing Monday. Funny how such a simple fix was literally sitting in the drawer next to the microtome all along! William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, March 13, 2009 12:28 PM To: Webb, Dorothy L Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] blades I will save a blade in a plastic 5-slide mailer. I usually use it for trimming so that I don't waste a new blade. I label the container with sharps info. Jennifer Webb, Dorothy L dorothy.l.w...@healthpartners.com Sent by: histonet-boun...@lists.utsouthwestern.edu 03/13/2009 10:03 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] blades Kind of a Friday type of question inasmuch as it could open some cutting remarks...how do others handle microtome blades that are not totally used? Our situation is that we use the more expensive teflon coated blades for certain tissues that are more delicate or difficult to obtain the most optimal sections and use the ceramic coated blades for routine microtomy. Obviously, the techs are oftentimes going to have a blade with an area that is still very usable and the dilemma is a safety issue as to where to place/store blades that are not ready to discard! Any good ideas??? Appreciate any kind ideas!! Thanks. This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
