Re: [Histonet] endothelial cell marker

2012-09-13 Thread Colleen Forster
I second Patsy! I have used this antibody with both AP and DAB and have 
had excellent results.


Colleen Forster
U of MN


On 9/13/2012 2:05 PM, Patsy Ruegg wrote:


By far the best ab for endothelial cells in mouse tissue is rat anti CD31
from Dianovo, it picks up the really early forming vessels better than any
other cd31 or F8 or SMA I have ever used.  We like it in AP/red.

Regards,
Patsy


Hi,
  My favorite by far is VonWillebrandt (spelling?) factor VIII. Dako has
a really good one (Cat# A0082) that works great in mouse tissue. CD31 is OK
but it is really finicky. CD34 works but F8 is easier and more reliable.

Amos


On Wed, Sep 5, 2012 at 1:01 PM,
wrote:


Message: 5
Date: Wed, 5 Sep 2012 10:04:58 +
From: "Bruijntjes, J.P. (Joost)" 
Subject: [Histonet] endothelial cell marker
To: "Histonet@lists.utsouthwestern.edu"
 
Message-ID:
 
Content-Type: text/plain; charset="us-ascii"

Hi all

Is anyone of you familiar with an antibody directed against endothelial
cells which can be applied on FFPE mouse tissues?

Best regards
Joost Bruijntjes


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RE: [Histonet] endothelial cell marker

2012-09-13 Thread Patsy Ruegg


By far the best ab for endothelial cells in mouse tissue is rat anti CD31
from Dianovo, it picks up the really early forming vessels better than any
other cd31 or F8 or SMA I have ever used.  We like it in AP/red.

Regards,
Patsy


Hi,
 My favorite by far is VonWillebrandt (spelling?) factor VIII. Dako has
a really good one (Cat# A0082) that works great in mouse tissue. CD31 is OK
but it is really finicky. CD34 works but F8 is easier and more reliable.

Amos


On Wed, Sep 5, 2012 at 1:01 PM,
wrote:

> Message: 5
> Date: Wed, 5 Sep 2012 10:04:58 +
> From: "Bruijntjes, J.P. (Joost)" 
> Subject: [Histonet] endothelial cell marker
> To: "Histonet@lists.utsouthwestern.edu"
> 
> Message-ID:
> 
> Content-Type: text/plain; charset="us-ascii"
>
> Hi all
>
> Is anyone of you familiar with an antibody directed against endothelial
> cells which can be applied on FFPE mouse tissues?
>
> Best regards
> Joost Bruijntjes
>
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