RE: [SPAM-HC] - RE: [Histonet] toe nails falling off slides - Email found in subject

2010-04-28 Thread Cazares, Ruth
Or you could soak them in 10% sodium hydroxide for 20-30 minutes prior to 
processing. My techs say they cut like butter!


Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
5145 North California Ave
Chicago, IL 60625

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maria Katleba
Sent: Tuesday, April 27, 2010 5:22 PM
To: zodia...@comcast.net; histonet@lists.utsouthwestern.edu
Subject: [SPAM-HC] - RE: [Histonet] toe nails falling off slides - Email found 
in subject

HOW TO CUT NAILS (or other difficult tissue) SO THAT THEY STAY on slide!

(1) Cut the slides and place on Plus(+) slide
(2) shake off excess water
(3) place slide immediately in plastic Coplin jar with 1-3 drops of formalin at 
bottom.
(4) place in hot oven for 5 mins (50-60 degrees)
(5) remove from Coplin jar under a hood as formalin drops are now carcinogenic 
vapour
(6) place slides in rack in oven so that the formalin moisture dries...
(7) slide is ready for use

Maria Katleba HT(ASCP), MS
Pathology Dept. Mgr.
Queen of the Valley Medical Center
707-294-9229 cell
707-252-4411 x3689

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
zodia...@comcast.net
Sent: Tuesday, April 27, 2010 12:17 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] toe nails falling off slides

To all,


I was wondering if anyone out there had any tips on how to keep toenail 
sections from falling off slides. We mount them on positive slides, air dry for 
about an hour but they still fall off. Any insight on the subject would be 
greatly appreciated.


Thanks,


Jenny
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RE: [Histonet] toe nails falling off slides - Email found in subject

2010-04-28 Thread histot...@imagesbyhopper.com
We have very good success in facing the block and soaking it on the  
water bath for 30-60 seconds and then allowing it to cool down on a  
very wet ice tray.  Ours cut like butter too!  :o)



On Apr 28, 2010, at 8:58 AM, Cazares, Ruth rcaza...@schosp.org  
wrote:


Or you could soak them in 10% sodium hydroxide for 20-30 minutes  
prior to processing. My techs say they cut like butter!



Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
5145 North California Ave
Chicago, IL 60625

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- 
boun...@lists.utsouthwestern.edu] On Behalf Of Maria Katleba

Sent: Tuesday, April 27, 2010 5:22 PM
To: zodia...@comcast.net; histonet@lists.utsouthwestern.edu
Subject: [SPAM-HC] - RE: [Histonet] toe nails falling off slides -  
Email found in subject


HOW TO CUT NAILS (or other difficult tissue) SO THAT THEY STAY on  
slide!


(1) Cut the slides and place on Plus(+) slide
(2) shake off excess water
(3) place slide immediately in plastic Coplin jar with 1-3 drops of  
formalin at bottom.

(4) place in hot oven for 5 mins (50-60 degrees)
(5) remove from Coplin jar under a hood as formalin drops are now  
carcinogenic vapour
(6) place slides in rack in oven so that the formalin moisture  
dries...

(7) slide is ready for use

Maria Katleba HT(ASCP), MS
Pathology Dept. Mgr.
Queen of the Valley Medical Center
707-294-9229 cell
707-252-4411 x3689

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- 
boun...@lists.utsouthwestern.edu] On Behalf Of zodia...@comcast.net

Sent: Tuesday, April 27, 2010 12:17 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] toe nails falling off slides

To all,


I was wondering if anyone out there had any tips on how to keep  
toenail sections from falling off slides. We mount them on positive  
slides, air dry for about an hour but they still fall off. Any  
insight on the subject would be greatly appreciated.



Thanks,


Jenny
___
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Histonet@lists.utsouthwestern.edu
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Notice from St. Joseph Health System:
Please note that the information contained in this message may be  
privileged and confidential and protected from disclosure.



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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***  Confidentiality Statement ***
This e-mail is intended only for the use of the individual or entity  
to which it is addressed and may contain information that is  
privileged and confidential. If the reader of this message is not  
the intended recipient, please notify the sender immediately by  
replying to this message and then delete it from your system. Any  
review, dissemination, distribution, or reproduction of this message  
by unintended recipients is strictly prohibited and may be subject  
to legal restriction.



Thank you for your cooperation.

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