Re: [Histonet] Control Slides (Ken M)
Here's the opinion of an 85 year old pathologist who in the course of his training spent a year doing histochemical research. In my experience, pathologists aren't allowed much input in the selection of controls. Anyone doing special stains needs to be able to evaluate the control slides for adequacy. Control tissue needs to be fixed and processed in the same way as the tissue being investigated. Some stains (like trichromes) need controls only to keep the inspectors happy, while others need a control run and looked at every time. Stains for micro-organisms require controls, for example. These controls should be tissue sections with active infection, not bugs embedded in gelatin. These controls are hard to get. Acid-fast stains require a control for the particular organism being looked for. Usually Mycobacterium tuberculosis. The best controls I've ever seen were lungs from rhesus monkeys with active tuberculosis, which a lot of them imported for research purposes had. The veterinary pathologists used to do these necropsies. Obviously one monkey can keep you supplied for a lifetime. Myco. leprae is a notoriously fickle stainer, and controls are hard to get, since very few animals can be infected with human leprosy. One leprous armadillo liver can supply a laboratory for many years. I think atypical mycobacteria usually get tuberculosis controls. The chromic acid methenamine silver stain is best controlled with the particular fungus you're looking for. The most demanding control is histoplasma in old inactive lung lesions. Candida is probably too easy. PAS really isn't a satisfactory fungus stain. Tissue Gram stains are vastly over-rated, and should be done away with. I think most people use a diseased appendix. If you're still doing a Giemsa or toluidine blue stain for Helicobacter, it should be controlled with a known positive for that organism. Amyloid controls have to be repeatedly tested to make sure they work. Human amyloidosis is a rare disease, and autopsy material these days is much rarer. I wonder if amyloid from animal controls has ever come into use. (Amyloidosis is supposed to be rather easily produced in mice.) I've never been able to get an answer to this question. Supposedly amyloid in sections still in wax on the slide (as controls are commonly prepared) isn't stable for more than a month. Bob Richmond Maryville TN I don't have enough technical experience with immunohistochemistry to have any ideas. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] control slides
Hey Cynthia- I’m curious what you used for an in-house control? I bet you have some in-house tissue that will work as well as the purchased controls. Try out a big piece or lung or colon adenocarcinoma from a resection, if you can. Val > On Jan 5, 2023, at 8:23 AM, Cynthia McGrady via Histonet > wrote: > > Hello. > Hoping someone can help me. Typically we order TAG 72 control slides from > Cell Marque.(our Docs are unhappy with our in-house control tissue, we've > tried). Cell Marque is on B/O no ETA. So my backup is Cancer Diagnostics, > they are 2-3 weeks out to fill our order. Any other companies out there > that sell TAG 72 control slides that I don't know about? Any help would be > greatly appreciated. Thanks..Cindy > Cindy McGrady > HT(ASCP) QIHC > Hurley Medical Center > One Hurley Plaza > Flint, Mi 48503 > 810-262-9138 > > -- > > > If you are not the intended > recipient(s), you are notified that any > disclosure, copying, distribution or > any action taken or omitted to be > taken in reliance on the contents of this > information is prohibited and may > be unlawful. If you receive this message in > error, or are not the named > recipient(s), please notify the sender, delete this > e-mail from your > computer, and destroy any copies in any form immediately. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] control slides
We actually just stared keeping a log for special stains, always for IHC. Our rule of thumb is that an IHC control slide cannot be oler than 6 months, although we go through most of them much faster. For the basic IHC, tonsil, fallopian tube etc we do not log the case information for IHC only specific antibody controls, HSV, HPV, etc. S. Paturzo TJHUH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Control Slides
There is an additional reason why I never bought control slides, and it refers to fixation/processing. How those tissues used as (+) control were fixed? How were they processed? It is the same problem when we received slides or blocks from another laboratory to be IHC tested as a consult. I was always sure that the tissues I used as (+) control were fixed and processed exactly the same as my patients' tissues and this is another advantage for making my own controls and avoiding (+) controls from other sources. René J. --- On Fri, 12/23/11, Nancy Schmitt nancy_schm...@pa-ucl.com wrote: From: Nancy Schmitt nancy_schm...@pa-ucl.com Subject: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Friday, December 23, 2011, 10:10 AM Peggy/Rene NSH has a tissue control bank - is there any reason why you couldn't contact them? We also make up our own control because we have access to tissue. The following link should take you to the NSH page with the information to get you going. http://www.nsh.org/committee-detail/1046 Merry Christmas Nancy Dubuque, IA From: Sherwood, Margaret msherw...@partners.org Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains?? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Control Slides
I've used http://www.americanmastertech.com/store/main.aspx?p=Categorybodyc=CV Only when I couldn't use what we had in the lab. Also some people might trade you if you have something they can use? Another thing I've noticed is occasionally vendors would request a control block for another client. I've always helped when I could so maybe you can ask for help from your vendors? Best of luck. Kim Sent from my iPhone On Dec 21, 2011, at 11:52 AM, Sherwood, Margaret msherw...@partners.org wrote: To all: I know this has come up before, but where do most people buy their (+) control slides for special stains? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Control Slides
To all: I know this has come up before, but where do most people buy their (+) control slides for special stains? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: Re: [Histonet] Control Slides
We get ours from Globe http://www.globescientific.com/ Their prices are reasonable, and we have not had any problems with either ihc or special stains. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, December 21, 2011 11:53 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Control Slides To all: I know this has come up before, but where do most people buy their (+) control slides for special stains? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Control Slides
I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. René J. --- On Wed, 12/21/11, Sherwood, Margaret msherw...@partners.org wrote: From: Sherwood, Margaret msherw...@partners.org Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Control Slides
Same here René, unless we can absolutely not find a positive case. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, December 21, 2011 11:05 AM To: histonet@lists.utsouthwestern.edu; MargaretSherwood Subject: Re: [Histonet] Control Slides I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. René J. --- On Wed, 12/21/11, Sherwood, Margaret msherw...@partners.org wrote: From: Sherwood, Margaret msherw...@partners.org Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Control Slides
How could you be sure those cases were positive if you didn't have a positive control before, the eternal palindrome... -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 21 December 2011 17:05 To: histonet@lists.utsouthwestern.edu; MargaretSherwood Subject: Re: [Histonet] Control Slides I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. René J. --- On Wed, 12/21/11, Sherwood, Margaret msherw...@partners.org wrote: From: Sherwood, Margaret msherw...@partners.org Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email is covered by the Kingston Hospital NHS Trust email disclaimer http://www.kingstonhospital.nhs.uk/trust-information/email-disclaimer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Control Slides
That is ideal Rene, but we are a research lab and don't run the same tissue all the time. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Wednesday, December 21, 2011 12:05 PM To: histonet@lists.utsouthwestern.edu; Sherwood, Margaret Subject: Re: [Histonet] Control Slides I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. René J. --- On Wed, 12/21/11, Sherwood, Margaret msherw...@partners.org wrote: From: Sherwood, Margaret msherw...@partners.org Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org http://us.mc657.mail.yahoo.com/mc/compose?to=msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://us.mc657.mail.yahoo.com/mc/compose?to=Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Control Slides
There was always a first positive that was used to evaluate the following and so on...so on... etc Besides, you know what to expect with a procedure and when you find it in some tissue, you know it. Elemental dear Watson. Merry Christmas René J. --- On Wed, 12/21/11, Essex, David david.es...@kingstonhospital.nhs.uk wrote: From: Essex, David david.es...@kingstonhospital.nhs.uk Subject: RE: [Histonet] Control Slides To: Rene J Buesa rjbu...@yahoo.com, histonet@lists.utsouthwestern.edu, MargaretSherwood msherw...@partners.org Date: Wednesday, December 21, 2011, 12:11 PM How could you be sure those cases were positive if you didn't have a positive control before, the eternal palindrome... -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 21 December 2011 17:05 To: histonet@lists.utsouthwestern.edu; MargaretSherwood Subject: Re: [Histonet] Control Slides I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. René J. --- On Wed, 12/21/11, Sherwood, Margaret msherw...@partners.org wrote: From: Sherwood, Margaret msherw...@partners.org Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email is covered by the Kingston Hospital NHS Trust email disclaimer http://www.kingstonhospital.nhs.uk/trust-information/email-disclaimer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Control Slides
Check with a colleague working in a hospital and ask for fresh tissue (for IHC) or (+) pathological cases. René J. --- On Wed, 12/21/11, Sherwood, Margaret msherw...@partners.org wrote: From: Sherwood, Margaret msherw...@partners.org Subject: RE: [Histonet] Control Slides To: Rene J Buesa rjbu...@yahoo.com, histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 12:15 PM That is ideal Rene, but we are a research lab and don't run the same tissue all the time. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Wednesday, December 21, 2011 12:05 PM To: histonet@lists.utsouthwestern.edu; Sherwood, Margaret Subject: Re: [Histonet] Control Slides I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. René J. --- On Wed, 12/21/11, Sherwood, Margaret msherw...@partners.org wrote: From: Sherwood, Margaret msherw...@partners.org Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Control Slides
I do not melt dry my cut control slides until i need to use them,= and
i do store them in boxes at 4c, i have recently run into this problem
= with blocks, so now i seal all my blocks with paraffin and store
them in th= e fridge as well.
Patsy
Original Message
Subject: RE:= [Histonet] Control Slides
From: Harrison, Sandra C. [1]sandra.harris...@va.gov
Date: Mon,= March 14, 2011 1:40 pm
To: Lee Peggy Wenk [2]lpw...@sbcglobal.net, Amador, Amanda
l=t;[3]aama...@ameripath.com,= lt;[4]histo...@lists.utso
uthwestern.edu
We dry the control slide at the same time tha= t we dry the tissue
being
stained, since controls are supposed to be han= dled in the same
manner as
the test tissue. That being said, I guess if = we were truly following
that rule, we would cut the control on the same = day we cut the test
sample. :-)
Other people say that, after they dry the slide, they dip the slide
in paraffin, to cover the
tissu= e,
so
that the air doesn't touch the tissue during the months before= the
slide
is
used in a stain.
-Original Message-
From: [5]histone= t-boun...@lists.utsouthwestern.edu
[[6]mailto:histonet-bounces@lists.utsouthwestern.e= du] On Behalf Of
Lee
Peggy Wenk
Sent: Thursday, March 10, 2= 011 6:39 PM
To: Amador, Amanda; [7]histonet@lists.utsouthwestern.edu
Subject: Re: [Histone= t] Control Slides
I've noticed that our spirochete control slides do= n't stain as
intense
starting about 3 months after they are sectioned, = and that they stop
staining by about 6 months. (This is with a silver s= tain.)
I've talked with other histotechs, and they say they've seen = the
same
phenomenon with AFB and Gram controls. (We use ours up too qui= ckly -
we
never have 6-12 months old control slides for these, so I can= 't
attest
to
this. )
Bancroft's book talks about this phenome= non with amyloid, and
suggests
that
oxidation of the tissue (protein= s) due to exposure to air may be the
cause.
I'm guessing the it prob= ably applies to the precut microorganism
control
slides, too.
We only cut enough slides for 6 months, and date them. Other people
say
they
put their cut control slides in a slide box with a lid after dryi= ng,
and
then place the box in the refrig, as cold slows down the che= mical
change,
and the lid keeps the moving air off the slide. Other = people say
that,
after
they dry the slide, they dip the slide in par= affin, to cover the
tissue,
so
that the air doesn't touch the tissue= during the months before the
slide
is
used in a stain. Just 3 sugge= stions to stop this problem.
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont= Hospital
Royal Oak, MI 48073
---= ---
From: Amador, Amanda [8]aama...@ameripath.com
Sent: Wednesday, March 09, 201= 1 10:22 PM
To: [9]= histonet@lists.utsouthwestern.edu
Subject: [Histonet] Control Sl= ides
Is there guidelines for how long special stains controls a= re good
for
once
they are cut? We have spirochetes for our Stei= ner that is from
2007
and
we are having issues.
= Amanda Amador, HT(ASCP)CM
AmeriPath | Histology Group Lead/Trainer= |2560 N Shadeland Ave,
Suite
A |
Indianapolis, IN 46219 | phon= e 317.275.8052 |
[10]aamador@= ameripath.commailto:[11]aam= a...@ameripath.com; |
[12]www.AmeriPath.comhttp://www.ameripath.c= om/;
___Histonet mailing list
[13]Histonet@lists.utsouthwestern.edu
[14]http://lists.utso= uthwestern.edu/mailman/listinfo/histonet
__= _
Histonet mailing list
[15]Histonet@lists.utsouthwestern.edu[16]htt
p://lists.utsouthwestern.edu/mailman/listinfo/histonet
_= __
Histonet mailing list
[17]Histonet@lists.utsouthwestern= .edu
[18]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
References
1. file://localhost/tmp/3Dma 2. file://localhost/tmp/3Dmailto 3.
3Dmailto:aama...@ameripath.com;
4. 3Dmailto:histonet@lists.utsouthwestern.edu;
5. 3Dmailto:histonet-boun...@lists.utsouthwestern.edu;
6. 3Dmailto:histonet-bounc 7. 3Dmailto:histonet@lists.utsouth 8.
3Dmailto:aamador@ame 9. 3Dmailto:histonet@lists.utsouthwestern.edu;
10. 3Dmailto:aama...@ameripath.com;
11. 3Dhttp://aama...@ameripath.com/
12. 3Dhttp://www.AmeriPath.coml=/
13. 3Dmailto:histo...@lists.uts 14. file://localhost/tmp/3Dh 15.
file://localhost/tmp/3Dmailto 16.
3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
17. =mailto:Histonet@lists.utsouthwestern.edu;
18.
3Dhttp://lists.utsouthwestern.edu/mailman/listinfo
RE: [Histonet] Control Slides
Hello, We have been storing our slides in small zip loc bags at -20 and have
data suggesting that slides stored in this fashion are reasonably well
preserved after 3- 5 years. This was done with TMA slides. Was not done for
special stain slides but for IHC.
Helen
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
pru...@ihctech.net
Sent: Tuesday, March 15, 2011 8:56 AM
To: Harrison,Sandra C.
Cc: histonet@lists.utsouthwestern.edu; Amador, Amanda
Subject: RE: [Histonet] Control Slides
I do not melt dry my cut control slides until i need to use them,=d
i do store them in boxes at 4c, i have recently run into this problem
=th blocks, so now i seal all my blocks with paraffin and store
them in th�ridge as well.
Patsy
Original Message
Subject: RE:=istonet] Control Slides
From: Harrison, Sandra C. [1]sandra.harris...@va.gov
Date: Mon,=rch 14, 2011 1:40 pm
To: Lee Peggy Wenk [2]lpw...@sbcglobal.net, Amador, Amanda
l=[3]aama...@ameripath.com,=;[4]histo...@lists.utso uthwestern.edu
We dry the control slide at the same time tha=e dry the tissue
being
stained, since controls are supposed to be han=ed in the same
manner as
the test tissue. That being said, I guess if = were truly following
that rule, we would cut the control on the same �y we cut the test
sample. :-)
Other people say that, after they dry the slide, they dip the slide
in paraffin, to cover the
tissu=
so
that the air doesn't touch the tissue during the months before=e
slide
is
used in a stain.
-Original Message-
From: [5]histone=boun...@lists.utsouthwestern.edu
[[6]mailto:histonet-bounces@lists.utsouthwestern.e=] On Behalf Of
Lee
Peggy Wenk
Sent: Thursday, March 10, 21 6:39 PM
To: Amador, Amanda; [7]histonet@lists.utsouthwestern.edu
Subject: Re: [Histone= Control Slides
I've noticed that our spirochete control slides do=t stain as
intense
starting about 3 months after they are sectioned, =d that they stop
staining by about 6 months. (This is with a silver s=in.)
I've talked with other histotechs, and they say they've seen =e
same
phenomenon with AFB and Gram controls. (We use ours up too qui=ly -
we
never have 6-12 months old control slides for these, so I can=
attest
to
this. )
Bancroft's book talks about this phenome=n with amyloid, and
suggests
that
oxidation of the tissue (protein= due to exposure to air may be the
cause.
I'm guessing the it prob�ly applies to the precut microorganism
control
slides, too.
We only cut enough slides for 6 months, and date them. Other people
say
they
put their cut control slides in a slide box with a lid after dryi=,
and
then place the box in the refrig, as cold slows down the che=cal
change,
and the lid keeps the moving air off the slide. Other =ople say
that,
after
they dry the slide, they dip the slide in par�fin, to cover the
tissue,
so
that the air doesn't touch the tissue=ring the months before the
slide
is
used in a stain. Just 3 sugge=ions to stop this problem.
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont=spital
Royal Oak, MI 48073
---=-
From: Amador, Amanda [8]aama...@ameripath.com
Sent: Wednesday, March 09, 2010:22 PM
To: [9]=sto...@lists.utsouthwestern.edu
Subject: [Histonet] Control Sl=es
Is there guidelines for how long special stains controls a= good
for
once
they are cut? We have spirochetes for our Stei=r that is from
2007
and
we are having issues.
=anda Amador, HT(ASCP)CM
AmeriPath | Histology Group Lead/Trainer=560 N Shadeland Ave,
Suite
A |
Indianapolis, IN 46219 | phon�17.275.8052 |
[10]aamador@=eripath.commailto:[11]aam�o...@ameripath.com; |
[12]www.AmeriPath.comhttp://www.ameripath.c=/;
___Histonet mailing list
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p://lists.utsouthwestern.edu/mailman/listinfo/histonet
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References
1. file://localhost/tmp/3Dma 2. file://localhost/tmp/3Dmailto 3.
3Dmailto:aama...@ameripath.com;
4. 3Dmailto:histonet@lists.utsouthwestern.edu;
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6. 3Dmailto:histonet-bounc 7. 3Dmailto:histonet@lists.utsouth 8.
3Dmailto:aamador@ame 9. 3Dmailto:histonet
RE: [Histonet] Control Slides
We dry the control slide at the same time that we dry the tissue being stained, since controls are supposed to be handled in the same manner as the test tissue. That being said, I guess if we were truly following that rule, we would cut the control on the same day we cut the test sample. :-) Other people say that, after they dry the slide, they dip the slide in paraffin, to cover the tissue, so that the air doesn't touch the tissue during the months before the slide is used in a stain. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, March 10, 2011 6:39 PM To: Amador, Amanda; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Control Slides I've noticed that our spirochete control slides don't stain as intense starting about 3 months after they are sectioned, and that they stop staining by about 6 months. (This is with a silver stain.) I've talked with other histotechs, and they say they've seen the same phenomenon with AFB and Gram controls. (We use ours up too quickly - we never have 6-12 months old control slides for these, so I can't attest to this. ) Bancroft's book talks about this phenomenon with amyloid, and suggests that oxidation of the tissue (proteins) due to exposure to air may be the cause. I'm guessing the it probably applies to the precut microorganism control slides, too. We only cut enough slides for 6 months, and date them. Other people say they put their cut control slides in a slide box with a lid after drying, and then place the box in the refrig, as cold slows down the chemical change, and the lid keeps the moving air off the slide. Other people say that, after they dry the slide, they dip the slide in paraffin, to cover the tissue, so that the air doesn't touch the tissue during the months before the slide is used in a stain. Just 3 suggestions to stop this problem. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -- From: Amador, Amanda aama...@ameripath.com Sent: Wednesday, March 09, 2011 10:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control Slides Is there guidelines for how long special stains controls are good for once they are cut? We have spirochetes for our Steiner that is from 2007 and we are having issues. Amanda Amador, HT(ASCP)CM AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A | Indianapolis, IN 46219 | phone 317.275.8052 | aama...@ameripath.commailto:aama...@ameripath.com | www.AmeriPath.comhttp://www.ameripath.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Control Slides
Amanda, You will lose antigenicity on the spirochete control slides after that long of a period. We usually cut no more than 10 in advance, and we keep them in the refrigerator. Hope this helps. Sheila HT (ASCP) Knoxville Dermatopathology Lab Knoxville, TN -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amador, Amanda Sent: Wednesday, March 09, 2011 10:22 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Control Slides Is there guidelines for how long special stains controls are good for once they are cut? We have spirochetes for our Steiner that is from 2007 and we are having issues. Amanda Amador, HT(ASCP)CM AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A | Indianapolis, IN 46219 | phone 317.275.8052 | aama...@ameripath.commailto:aama...@ameripath.com | www.AmeriPath.comhttp://www.ameripath.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Control Slides
Sheila, I believe that Amanda is referring to control slides cut for the Steiner Modification of the Warthin-Starry stain not for immunohistochemistry. I would be surprised if the control slides for the Steiner's would go off. It is possible that the technique has gone out of control. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Thursday, 10 March 2011 11:53 PM To: 'Amador, Amanda'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Control Slides Amanda, You will lose antigenicity on the spirochete control slides after that long of a period. We usually cut no more than 10 in advance, and we keep them in the refrigerator. Hope this helps. Sheila HT (ASCP) Knoxville Dermatopathology Lab Knoxville, TN -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amador, Amanda Sent: Wednesday, March 09, 2011 10:22 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Control Slides Is there guidelines for how long special stains controls are good for once they are cut? We have spirochetes for our Steiner that is from 2007 and we are having issues. Amanda Amador, HT(ASCP)CM AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A | Indianapolis, IN 46219 | phone 317.275.8052 | aama...@ameripath.commailto:aama...@ameripath.com | www.AmeriPath.comhttp://www.ameripath.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Control Slides
I've noticed that our spirochete control slides don't stain as intense starting about 3 months after they are sectioned, and that they stop staining by about 6 months. (This is with a silver stain.) I've talked with other histotechs, and they say they've seen the same phenomenon with AFB and Gram controls. (We use ours up too quickly - we never have 6-12 months old control slides for these, so I can't attest to this. ) Bancroft's book talks about this phenomenon with amyloid, and suggests that oxidation of the tissue (proteins) due to exposure to air may be the cause. I'm guessing the it probably applies to the precut microorganism control slides, too. We only cut enough slides for 6 months, and date them. Other people say they put their cut control slides in a slide box with a lid after drying, and then place the box in the refrig, as cold slows down the chemical change, and the lid keeps the moving air off the slide. Other people say that, after they dry the slide, they dip the slide in paraffin, to cover the tissue, so that the air doesn't touch the tissue during the months before the slide is used in a stain. Just 3 suggestions to stop this problem. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -- From: Amador, Amanda aama...@ameripath.com Sent: Wednesday, March 09, 2011 10:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control Slides Is there guidelines for how long special stains controls are good for once they are cut? We have spirochetes for our Steiner that is from 2007 and we are having issues. Amanda Amador, HT(ASCP)CM AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A | Indianapolis, IN 46219 | phone 317.275.8052 | aama...@ameripath.commailto:aama...@ameripath.com | www.AmeriPath.comhttp://www.ameripath.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
