Re: [Histonet] CD31 working protocol
BD Biosciences has a great rodent CD31 for mouse and rat - however you need to use their Zinc Fixative. It is well worth the results!! Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: port...@msu.edu Web: www.humanpathology.msu.edu - Original Message - From: Zerfas, Patricia (NIH/OD/ORS) [E] zerf...@ors.od.nih.gov To: histonet@lists.utsouthwestern.edu Sent: Monday, August 10, 2009 2:50 PM Subject: [Histonet] CD31 working protocol Dear Listservers, I have tried MANY protocols and I am unable to obtain a working protocol for the CD31 antibody. I have tried both the CD31 rat monoclonal and a CD31 rabbit polyclonal. Thanks in advance for your help. Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD31 working protocol
Biocare has a great CD31 for mouse and rat too. You do need the kit and their primary but you can use formalin fixed samples with great results! Colleen Forster U of MN Amy Porter wrote: BD Biosciences has a great rodent CD31 for mouse and rat - however you need to use their Zinc Fixative. It is well worth the results!! Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: port...@msu.edu Web: www.humanpathology.msu.edu - Original Message - From: Zerfas, Patricia (NIH/OD/ORS) [E] zerf...@ors.od.nih.gov To: histonet@lists.utsouthwestern.edu Sent: Monday, August 10, 2009 2:50 PM Subject: [Histonet] CD31 working protocol Dear Listservers, I have tried MANY protocols and I am unable to obtain a working protocol for the CD31 antibody. I have tried both the CD31 rat monoclonal and a CD31 rabbit polyclonal. Thanks in advance for your help. Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD31 working protocol
Biocare has a CD31 anti mouse that does stain FFPE.I personally think nothing will ever compare with the Santa Cruz goat that no longer is available - but Biocare does work. Zerfas, Patricia (NIH/OD/ORS) [E] zerf...@ors.od.nih.gov Sent by: histonet-boun...@lists.utsouthwestern.edu 08/10/2009 01:50 PM To 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu cc Subject [Histonet] CD31 working protocol Dear Listservers, I have tried MANY protocols and I am unable to obtain a working protocol for the CD31 antibody. I have tried both the CD31 rat monoclonal and a CD31 rabbit polyclonal. Thanks in advance for your help. Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD31 working protocol
Hi Patricia, What tissue (animal / human) are you using? How has it been fixed / processed? If you are using 10%NBF fixed, paraffin-embedded mouse tissue, you can use Biocare's CD31 with a simple indirect protocol. I come in with my primary (overnight at 5ug/ml) after Prot. K digestion (DAKO ready-to-use), and then come in with a secondary Alexa fluor 488 against my primary. It work really well. I have also used it for frozen sections. I hope this helps. Regards. Gustave Gustave T. Hebert Research Scientist I Metabolic Disease Research Wyeth Research 200 CambridgePark Drive Cambridge, MA 02140 --- On Mon, 8/10/09, Zerfas, Patricia (NIH/OD/ORS) [E] zerf...@ors.od.nih.gov wrote: From: Zerfas, Patricia (NIH/OD/ORS) [E] zerf...@ors.od.nih.gov Subject: [Histonet] CD31 working protocol To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Date: Monday, August 10, 2009, 2:50 PM Dear Listservers, I have tried MANY protocols and I am unable to obtain a working protocol for the CD31 antibody. I have tried both the CD31 rat monoclonal and a CD31 rabbit polyclonal. Thanks in advance for your help. Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD31 working protocol
Hi Patricia, I use an antibody from Spring Biosciences Cat#M3384 to stain our human xenograft tumors as well as human tissue. Antibody will also stain mouse tissue. Very nice clean crisp stain. At least when we use it on our Bond IHC stainiers. Thanks and let me know if you need more information. dusko From: Zerfas, Patricia (NIH/OD/ORS) [E] zerf...@ors.od.nih.gov To: dusko trajkovic dunat...@sbcglobal.net Sent: Monday, August 10, 2009 12:07:29 PM Subject: RE: [Histonet] CD31 working protocol Dusko, I am using mouse tissue. Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 From:dusko trajkovic [mailto:dunat...@sbcglobal.net] Sent: Monday, August 10, 2009 3:06 PM To: Zerfas, Patricia (NIH/OD/ORS) [E] Subject: Re: [Histonet] CD31 working protocol What species are you trying to stain? thanks Dusko From:Zerfas, Patricia (NIH/OD/ORS) [E] zerf...@ors.od.nih.gov To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Monday, August 10, 2009 11:50:50 AM Subject: [Histonet] CD31 working protocol Dear Listservers, I have tried MANY protocols and I am unable to obtain a working protocol for the CD31 antibody. I have tried both the CD31 rat monoclonal and a CD31 rabbit polyclonal. Thanks in advance for your help. Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda , MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
