Re: [Histonet] Deleting extraneous parts of posts when replying
My brief experience with paraffin and fluorescence (DAPI and EGFP) is that the paraffin embedding process destroys it. Regards, Merced --On Tuesday, March 02, 2010 3:25 PM -0600 Fabrice gankam gan...@googlemail.com wrote: Hi, Just wondering if anyone has use hydroethinide to detect the free radicals (ROS) in the CNS or anyother tissue of rats after 4% formallin fixation and parrafin embedding. I wanted to used the hydroethidine on paraffin sections. I wonder if the fluorescence is lost by fixation (12hrs in 4% PAF) and paraffin embedding plus deparaffination. All the papers I reviewed used hydroethidine on frozen section but our facility does not have vibratome or cryostat. Please help Please Help. Fabrice ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Deleting extraneous parts of posts when replying
given how many people cant even figure out how to properly unsubscribe from this list, i fear your request might be in vain ;-) Mark Elliott wrote: Just a friendly reminder that if you are responding to a question to please delete out everything but the pertinent information, especially if you are receiving/responding in Digest mode. If you don't cut out the irrelevant posts it makes it extremely hard to find the new stuff. Some of the Digest versions that have come out in the last few days have Digests within Digests within Digests so it makes the email really long and you can't find the new stuff in amongst the old. Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Deleting extraneous parts of posts when replying
Hi, Just wondering if anyone has use hydroethinide to detect the free radicals (ROS) in the CNS or anyother tissue of rats after 4% formallin fixation and parrafin embedding. I wanted to used the hydroethidine on paraffin sections. I wonder if the fluorescence is lost by fixation (12hrs in 4% PAF) and paraffin embedding plus deparaffination. All the papers I reviewed used hydroethidine on frozen section but our facility does not have vibratome or cryostat. Please help Please Help. Fabrice ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
