Agree; I guess the difference is that FNAC and Micro samples haven't been
subjected to processing like the tissue block sections. The tissue stains have
been shown to work on something that has been fixed, dehydrated, set in hot
wax, cut, rehydrated and then stained. The FNAC and Micro samples may or may
not have been fixed (although I concede air drying is a form of fixation) and
the stains used on them have been shown to work.
The logic that these techniques are interchangeable is not only flawed but
(oxy)moronic.
Kemlo Rogerson
e-mail kemloroger...@nhs.net if not at work.
DD 01934 647057 or extension 3311 Mob 07749 754194;
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: 06 May 2009 17:24
To: histonet@lists.utsouthwestern.edu; Angela Bitting
Subject: Re: [Histonet] Gram AFB staining FNA smears
The doc is wrong, otherwise your histology sections to be stained with Gram
AFB should also be sent to micro. Perhaps you will not have to do them anymore.
Your doc's reasoning is purely oxymoronic.
René J.
--- On Wed, 5/6/09, Angela Bitting akbitt...@geisinger.edu wrote:
From: Angela Bitting akbitt...@geisinger.edu
Subject: [Histonet] Gram AFB staining FNA smears
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, May 6, 2009, 9:22 AM
The subject of staining FNA smears with AFB and Gram stains in Histology vs in
Micro came up today. Is there a reason that FNAs can't be stained the same way
we stain our tissue sections? One of our docs was under the impression that
it's not acceptable to use the staining methods we use in our Histology lab.
I don't know what method Micro labs use, so I was hoping someone could shed
some light on this subject for me.
Thanks, as always,
Angie
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
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