Re: [Histonet] Old slides.
Hi We re-Coverslipper a number of sections which had peeled off on the tape as the tape dried and curled. We cut off excess tape using scissors; placed a fresh coverslip flat; put a streak of mounting medium on he coverslip; use a forceps to orientate the tissue + margin of tape; number a slide, dip it in Xylene place on a slope bring on top of coverslip-section-mounting medium; turn the slide-section- coverslip to face coverslip up; leave horizontal to dry (eg overnight). Worth a try, doing one first. Bernie, St Vincent's, Dublin, Ireland On 9 Mar 2015, at 19:41, Bernice Frederick b-freder...@northwestern.edu wrote: Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Old slides.
Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com mailto:jmcgo...@clinlab.com www.clinlab.com http://www.clinlab.com -Original message- From:Bernice Frederick b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu Sent: Monday, March 9, 2015 1:51 PM To: histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu Subject: [Histonet] Old slides. Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu mailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Old slides.
Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one. John Kiernan Anatomy Cell Biology, UWO London, Canada = = = On 09/03/15, Jason McGough jmcgo...@clinlab.com wrote: Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com mailto:jmcgo...@clinlab.com jmcgo...@clinlab.com www.clinlab.com http://www.clinlab.com -Original message- From:Bernice Frederick b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu b-freder...@northwestern.edu Sent: Monday, March 9, 2015 1:51 PM To: histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] Old slides. Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu mailto:Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
