Re: [Histonet] plural fluid prep

[Beth Cox via Histonet]

Hi Michelle,

When I was in my Cytology training, we did an experiment to answer just 
that question.  We started with a 2 liter jug of plural fluid with 
nothing added, and we stored in in the fridge.  At first we tested it 
every day to see if there was any degeneration, then we got tired of 
that and tested twice a week, then once a week. After 4 months it was 
still just fine, and we threw it away because we were tired of testing 
it (and it was the end of the semester!).   It seems that the cells are 
happy in their natural habitat and refrigeration slowed/prevented any 
bacterial growth.


So  my advice is:  don't add anything to it, keep it in the fridge, and 
it will last many months!!


Beth Cox, SCT/HTL(ASCP)QIHC

From: Michelle Jamison
To:"histonet@lists.utsouthwestern.edu"
   
Subject: [Histonet] plural fluid prep
Message-ID:
Content-Type: text/plain; charset=utf-8; format=flowed

Hi All,
I work in a research lab where we are using plural fluid to proof a
method. Recently we have received some, and are unsure how long it will
be ok in the fridge, or if we should add some preservation fluid to it.
Can anyone advise please.


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Re: [Histonet] plural fluid prep

[stephanie h via Histonet]

Can you spin it down and make into blocks? Does it have to be fresh PF?

> On Dec 22, 2019, at 10:45 AM, Amos Brooks via Histonet 
>  wrote:
> 
> 
>> 
>> 
>> 
>> Hi Michelle,
>Pleural fluid lasts a fairly long time if you don't centrifuge it and
> leave it in suspension. Of course it will last even longer if properly
> fixed. I have returned to a stored (unfixed) pleural fluid for
> retrospective testing up to a week later.
> The longer you let it sit though, the more complications can occur
> like bacterial growth and further call division or death depending on
> what's going on in the fluid.
> 
> Amos Brooks
> 
>> 
>> Message: 7
>> Date: Wed, 18 Dec 2019 10:51:40 -0700
>> From: Michelle Jamison 
>> To: "histonet@lists.utsouthwestern.edu"
>>
>> Subject: [Histonet] plural fluid prep
>> Message-ID: 
>> Content-Type: text/plain; charset=utf-8; format=flowed
>> 
>> Hi All,
>> I work in a research lab where we are using plural fluid to proof a
>> method. Recently we have received some, and are unsure how long it will
>> be ok in the fridge, or if we should add some preservation fluid to it.
>> Can anyone advise please.
>> 
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Re: [Histonet] plural fluid prep

[John Garratt via Histonet]

The cells have been living quite nicely in a natural culture medium at 37c so 
putting the pleural fluid at 4c will preserve the cells for a number of days. I 
do not think I would want to go past 3 to 4 days, but that is just the limit of 
my experience revisiting old ascitic fluid specimens.
You could use alcohol to preserve the specimen longer but not knowing what your 
experiment is I am loath to offer an opinion on that.

John

Sent from ProtonMail Mobile

On Wed, Dec 18, 2019 at 9:51 AM, Michelle Jamison via Histonet 
 wrote:

> Hi All,
> I work in a research lab where we are using plural fluid to proof a
> method. Recently we have received some, and are unsure how long it will
> be ok in the fridge, or if we should add some preservation fluid to it.
> Can anyone advise please.
> Thank you in advance, and Happy Holidays to all!!
> --
>
> Best of all things to you,
>
> Michelle Jamison
>
> //
>
> Tel : +1.435.752.6011 Ext. 435.227.1474
>
> m.jami...@elitechgroup.com  • www.elitechgroup.com
> 
>
> 
>
> Logo
>
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