[MARMAM] New publication: Capture Myopathy and Stress Cardiomyopathy in a Live-Stranded Risso’s Dolphin (Grampus griseus) in Rehabilitation

[Kita Câmara]

Dear MARMAMers,

We are delighted to announce the publication of the following Case Report in 
Animals:

Capture Myopathy and Stress Cardiomyopathy in a Live-Stranded Risso’s Dolphin 
(Grampus griseus) in Rehabilitation

Nakita Câmara1, Eva Sierra1, Antonio Fernández1, Manuel Arbelo1, Yara Bernaldo 
de Quirós1, Marina Arregui1, Francesco Consoli1,2 and Pedro Herráez1


  1.
Veterinary Histology and Pathology, Institute of Animal Health and Food Safety 
(IUSA), Veterinary School, University of Las Palmas de Gran Canaria, Arucas, 
35416 Las Palmas de Gran Canaria, Spain
  2.
Department of Neuroscience, Imaging and Clinical Sciences, University G. 
D’Annunzio, 66100 Chieti, Italy

DOI: 10.3390/ani10020220


Abstract

Capture myopathy (CM) is described in wild animals as a metabolic syndrome 
resulting from the extreme stress suffered during and after capture, handling, 
restraint, and transport. Although CM has been characterized in many species of 
cetaceans, descriptions of cardiac injury—an important component of this 
syndrome, and, according to previous authors, comparable to the existing human 
pathology so-called stress cardiomyopathy (SCMP)—are still rare. Therefore, the 
main aim of this report is to illustrate, for the first time, the biochemical 
analysis, and gross, histopathological, histochemical and immunohistochemical 
features of CM, and more specifically of the SCMP involved in this syndrome, 
caused by the live-stranding and consequent rehabilitation attempt, for a 
certain period of time, in a juvenile male Risso’s dolphin (Grampus griseus). 
The animal presented elevated values of creatine kinase, cardiac troponin I and 
blood urea nitrogen, with some variations during the rehabilitation period. 
Histologically, we detected vascular changes and acute degenerative lesions 
analogous to the ones observed in humans with SCMP. We consider this study to 
be an important contribution to the study of cetaceans since it could help in 
decision-making and treatment procedures during live-strandings and improve 
conservation efforts by reducing the mortality of these animals.



The paper is available online at https://www.mdpi.com/2076-2615/10/2/220.

If you have any questions you can contact me at  kita_cam...@hotmail.com or 
nakita.camara...@alu.ulpgc.es

Best regards,


Nakita Câmara
DVM, PhD Student

Centro Atlántico de Investigación de Cetáceos,
Instituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA), 
Universidad de Las Palmas de Gran Canaria (ULPGC).

Campus Universitario Cardones de Arucas, Trasmontaña s/n
35416 Arucas, Las Palmas de Gran Canaria, España

Telephone: +34 928 45 97 16
Mobile: +34 611 05 00 08

Email: nakita.camara...@alu.ulpgc.es
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[MARMAM] New publication: Increased Plasma Cardiac Troponin I in Live-Stranded Cetaceans: Correlation with Pathological Findings of Acute Cardiac Injury

[Kita Câmara]

Dear MARMAMers,

We are delighted to announce the publication of the following article in 
Scientific Reports:

Increased Plasma Cardiac Troponin I in Live-Stranded Cetaceans: Correlation 
with Pathological Findings of Acute Cardiac Injury

Nakita Câmara1, Eva Sierra1, Antonio Fernández1, Manuel Arbelo1, Marisa 
Andrada1, Antonio Espinosa de los Monteros1,  and Pedro Herráez1


  1.
Veterinary Histology and Pathology, Institute of Animal Health and Food Safety 
(IUSA), Veterinary School, University of Las Palmas de Gran Canaria, Arucas, 
35416 Las Palmas de Gran Canaria, Spain

DOI: 10.1038/s41598-020-58497-3


Abstract

Capture myopathy (CM), is a syndrome that occurs as the result of the stress 
during and after capture, handling, restraint, and transport of wild animals. 
Although CM has been described for many species of cetaceans, characterization 
of the acute cardiac injury - an important component of this syndrome - are 
still scarce. In this study, we firstly estimated a normal range for cardiac 
troponin I (cTnI) on cetaceans. Here, through biochemical analysis (especially 
of cTnI) and histopathological, histochemical, and immunohistochemical 
correlations with decreased troponin immunolabelling, we studied the cardiac 
injury in live-stranded cetaceans. Nine cetaceans which stranded alive on the 
Canary Islands (January 2016 - June 2019) were included in this study. Sampled 
individuals presented elevated values of plasma cTnI, which were correlated to 
histopathological lesions comprised of vascular changes and acute degenerative 
lesions. Immunohistochemically, injured cardiomyocytes showed a decreased 
intrafibrillar troponin immunoreaction. This is the first attempt to establish 
a normal baseline range for cTnI in cetaceans, and the first study comparing 
plasma biomarkers values with histopathological and immunohistochemical 
findings. This approach allowed us to demonstrate the degree of cardiac damage 
as a result of injury, consistent with ischemia–reperfusion lesions. The 
knowledge gained here could improve decision-making procedures during stressful 
situations, mainly in live-strandings, handling, and rehabilitation, thereby 
reducing the mortality of cetaceans.



The paper is available online at 
https://www.nature.com/articles/s41598-020-58497-3#citeas
[https://media.springernature.com/m685/springer-static/image/art%3A10.1038%2Fs41598-020-58497-3/MediaObjects/41598_2020_58497_Fig1_HTML.png]
Increased Plasma Cardiac Troponin I in Live-Stranded Cetaceans: Correlation 
with Pathological Findings of Acute Cardiac 
Injury
www.nature.com


If you have any questions you can contact me at  kita_cam...@hotmail.com or 
nakita.camara...@alu.ulpgc.es

Best regards,


Nakita Câmara
DVM, PhD Student

Centro Atlántico de Investigación de Cetáceos,
Instituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA), 
Universidad de Las Palmas de Gran Canaria (ULPGC).

Campus Universitario Cardones de Arucas, Trasmontaña s/n
35416 Arucas, Las Palmas de Gran Canaria, España

Telephone: +34 928 45 97 16
Mobile: +34 611 05 00 08

Email: nakita.camara...@alu.ulpgc.es
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[MARMAM] New publication: Skeletal and Cardiac Rhabdomyolysis in a Live-Stranded Neonatal Bryde's Whale With Fetal Distress

[Kita Câmara]

Dear MARMAMers,

We are delighted to announce the publication of the following Case Report in 
Frontiers in Veterinary Science:

Skeletal and Cardiac Rhabdomyolysis in a Live-Stranded Neonatal Bryde's Whale 
With Fetal Distress

Nakita Câmara1, Eva Sierra1, Antonio Fernández1, Cristian Manuel 
Suárez-Santana1, Raquel Puig-Lozano1, Manuel Arbelo1 and Pedro Herráez1


  1.
Department of Veterinary Histology and Pathology, Veterinary School, Institute 
of Animal Health and Food Safety, University of Las Palmas de Gran Canaria, Las 
Palmas de Gran Canaria, Spain

DOI: 10.3389/fvets.2019.00476


Abstract

The main objective of wildlife forensic investigation is to recognize 
pathologic changes and cause of death. Even though it may not always be 
possible to determine the specific illness and/or etiology, the description and 
subsequent interpretation of the injuries provide an invaluable understanding 
of pathology in cetacean post-mortem investigations. Although pathological 
studies have been previously reported in various cetacean species, such 
descriptions of the infraorder Mysticeti remain rare. A live-stranded neonatal 
Bryde's whale (Balaenoptera edeni) which subsequently died soon after the 
stranding, was assessed by physical exam, blood examination, gross necropsy 
evaluation, histopathology, and immunohistochemistry. It presented with 
elevated serum levels of creatine kinase, cardiac troponin I, urea, and 
creatinine. Microscopically, we observed keratin spicules (squamous epithelial 
cells) and areas of atelectasis in the lungs. Acute degeneration in the 
myocytes and cardiomyocytes were comparable to the findings previously 
described in cases of capture myopathy in live-stranded cetaceans. 
Immunohistochemistry biomarkers such as myoglobin, fibrinogen, and troponin 
were analyzed. Skeletal and myocardial damage has been documented in several 
cetacean species. However, this is the first reported case of skeletal and 
cardiac rhabdomyolysis associated with live-stranding in a newborn Bryde's 
whale that suffered from fetal distress.



The paper is available online at 
https://www.frontiersin.org/articles/10.3389/fvets.2019.00476/full.

If you have any questions you can contact me at  kita_cam...@hotmail.com or 
nakita.camara...@alu.ulpgc.es

Best regards,


Nakita Câmara
DVM, PhD Student

Centro Atlántico de Investigación de Cetáceos,
Instituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA), 
Universidad de Las Palmas de Gran Canaria (ULPGC).

Campus Universitario Cardones de Arucas, Trasmontaña s/n
35416 Arucas, Las Palmas de Gran Canaria, España

Telephone: +34 928 45 97 16
Mobile: +34 611 05 00 08

Email: nakita.camara...@alu.ulpgc.es


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[MARMAM] New publication: Stress Cardiomyopathy in stranded cetaceans: a histological, histochemical and immunohistochemical study

[Kita Câmara]

Dear MARMAMers,

We are delighted to announce the publication of the following article in 
Veterinary Record:

Stress cardiomyopathy in stranded cetaceans: a histological, histochemical and 
immunohistochemical study

Nakita Câmara1, Eva Sierra1, Carolina Fernández-Maldonado2, Antonio Espinosa de 
los Monteros1, Manuel Arbelo1, Antonio Fernández1 and Pedro Herráez1


  1.
Departamento de Histología y Patología Animal, Instituto Universitario de 
Sanidad Animal y Seguridad Alimentaria (IUSA), Universidad de Las Palmas de 
Gran Canaria Facultad de Veterinaria, Arucas, Spain
  2.
Seashore Environment and Fauna, Cádiz, Spain

DOI: 10.1136/vr.105562


Abstract

Background Free-living cetaceans are exposed to a wide variety of stressful 
situations, including live stranding and interaction with human beings (capture 
myopathy), vessel strikes, and fishing activities (bycatch), which affect their 
wellbeing and potentially lead to stress cardiomyopathy (SCMP).
Methods Here, the authors aimed to characterise SCMP of stranded cetaceans as 
an injury resulting from extreme stress responses, based on pathological 
analyses (histological, histochemical and immunohistochemical). Specifically, 
the authors examined heart samples from 67 cetaceans found ashore (48 live 
strandings, seven dead from ship collision and 12 dead from bycatch) on the 
coast of Spain, more specifically in the Canary Islands from 2000 to 2016 and 
Andalusia from 2011 to 2014.
Results The microscopic findings were characterised by vascular changes, acute 
or subacute cardiac degenerative necrotic lesions, interstitial myoglobin 
globules, and infiltration of inflammatory cells. Immunohistochemically, 
cardiac troponin I, cardiac troponin C and myoglobin were depleted, along with 
fibrinogen being expressed in the degenerated/necrotic cardiomyocytes. A 
perivascular pattern was also identified and described in the damaged 
cardiomyocytes.
Conclusions This study advances current knowledge about the pathologies of 
cetaceans and their implications on conserving this group of animals by 
reducing mortality and enhancing their treatment and subsequent rehabilitation 
to the marine environment.


The paper is available online at 
http://veterinaryrecord.bmj.com/cgi/content/full/vr.105562 or you can contact 
me for a pdf version or for any questions at  kita_cam...@hotmail.com or 
nakita.camara...@alu.ulpgc.es

Best regards,

Nakita Câmara


Nakita Câmara
DVM, PhD Student

Centro Atlántico de Investigación de Cetáceos,
Instituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA), 
Universidad de Las Palmas de Gran Canaria (ULPGC).

Campus Universitario Cardones de Arucas, Trasmontaña s/n
35416 Arucas, Las Palmas de Gran Canaria, España

Telephone: +34 928 45 97 16
Mobile: +34 611 05 00 08

Email: nakita.camara...@alu.ulpgc.es

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