[R-sig-phylo] PGLS with non-ultrametric tree

2015-07-16 Thread Solomon Chak
Dear all,

What are the pros and cons of the two methods to do pgls with a
non-ultrametric tree? Many thanks!

1) Convert the additive tree to ultrametric using penalized likelihood
(Sanderson 2002) with ape:: chronopl after cross-validation to find the
best lambda.

2) Use weighted least-squares in which gls(y ~ x,
correlation=corPagel(value=0.1, phy=phy, fixed=F),
weights=varFixed(~tip.heights),
data=dat)

Cheers,
Solomon Chak

---
*Solomon **Tin Chi **Chak*
Ph.D. Candidate
Tel: (804) 684-7484
Marine Biodiversity Laboratory
http://www.vims.edu/research/units/labgroups/marine_biodiversity/index.php
Virginia Institute of Marine Science http://www.vims.edu/
College of William and Mary http://www.wm.edu/
PO Box 1346 / Rt. 1208 Greate Rd., Gloucester Pt, VA 23062, USA

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Re: [R-sig-phylo] PGLS with non-ultrametric tree

2015-07-16 Thread Theodore Garland Jr
Dear Solomon,

I don;t know where you are trying to go with this, but any proper 
implementation of PGLS (I am not talking about methods that transform the 
branch lengths with things like Grafen's rho, Pagel's lambda or OU models) 
should be able to use any tree of any shape.  This is the same as for 
phylogenetically independent contrasts.

Cheers,
Ted

Theodore Garland, Jr., Professor
Department of Biology
University of California, Riverside
Riverside, CA 92521
Office Phone:  (951) 827-3524
Facsimile:  (951) 827-4286 (not confidential)
Email:  tgarl...@ucr.edu
http://www.biology.ucr.edu/people/faculty/Garland.html
http://scholar.google.com/citations?hl=enuser=iSSbrhwJ

Director, UCR Institute for the Development of Educational Applications

Editor in Chief, Physiological and Biochemical Zoology

Fail Lab: Episode One
http://testtube.com/faillab/zoochosis-episode-one-evolution
http://www.youtube.com/watch?v=c0msBWyTzU0


From: R-sig-phylo [r-sig-phylo-boun...@r-project.org] on behalf of Solomon Chak 
[tc...@vims.edu]
Sent: Thursday, July 16, 2015 3:31 AM
To: r-sig-phylo@r-project.org
Subject: [R-sig-phylo] PGLS with non-ultrametric tree

Dear all,

What are the pros and cons of the two methods to do pgls with a
non-ultrametric tree? Many thanks!

1) Convert the additive tree to ultrametric using penalized likelihood
(Sanderson 2002) with ape:: chronopl after cross-validation to find the
best lambda.

2) Use weighted least-squares in which gls(y ~ x,
correlation=corPagel(value=0.1, phy=phy, fixed=F),
weights=varFixed(~tip.heights),
data=dat)

Cheers,
Solomon Chak

---
*Solomon **Tin Chi **Chak*
Ph.D. Candidate
Tel: (804) 684-7484
Marine Biodiversity Laboratory
http://www.vims.edu/research/units/labgroups/marine_biodiversity/index.php
Virginia Institute of Marine Science http://www.vims.edu/
College of William and Mary http://www.wm.edu/
PO Box 1346 / Rt. 1208 Greate Rd., Gloucester Pt, VA 23062, USA

[[alternative HTML version deleted]]

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Re: [R-sig-phylo] PGLS with non-ultrametric tree

2015-07-16 Thread Solomon Chak
Hi Dr. Garland,

I got the impression that an ultrametric tree is needed for comparative
analysis from this book http://www.springer.com/us/book/9783662435496ch.
2 pg. 38: most comparative analyses assume that the tree is ultrametric,
as the majority of analyses deal with evolution of phenotypic traits of
extant species with the underlying assumption is that the time available
for phenotypic evolution is the same for all taxa.

I understand that the methodology of PGLS can use any tree form, I suppose
there're different assumptions if I used an additive tree or an ultrametric
tree. Could you guide me to some references that explicitly discuss about
this?

Is it necessary to use an ultrametric tree If I want to estimate lambda in
the residual error of the gls and do a branch length transformation as Revell
2010
http://onlinelibrary.wiley.com/doi/10./j.2041-210X.2010.00044.x/pdf
suggested?

Many thanks!

Cheers,
Solomon Chak

---
*Solomon **Tin Chi **Chak*
Ph.D. Candidate
Tel: (804) 684-7484
Marine Biodiversity Laboratory
http://www.vims.edu/research/units/labgroups/marine_biodiversity/index.php
Virginia Institute of Marine Science http://www.vims.edu/
College of William and Mary http://www.wm.edu/
PO Box 1346 / Rt. 1208 Greate Rd., Gloucester Pt, VA 23062, USA


On Thu, Jul 16, 2015 at 2:55 PM, Theodore Garland Jr 
theodore.garl...@ucr.edu wrote:

 Dear Solomon,

 I don;t know where you are trying to go with this, but any proper
 implementation of PGLS (I am not talking about methods that transform the
 branch lengths with things like Grafen's rho, Pagel's lambda or OU models)
 should be able to use any tree of any shape.  This is the same as for
 phylogenetically independent contrasts.

 Cheers,
 Ted

 Theodore Garland, Jr., Professor
 Department of Biology
 University of California, Riverside
 Riverside, CA 92521
 Office Phone:  (951) 827-3524
 Facsimile:  (951) 827-4286 (not confidential)
 Email:  tgarl...@ucr.edu
 http://www.biology.ucr.edu/people/faculty/Garland.html
 http://scholar.google.com/citations?hl=enuser=iSSbrhwJ

 Director, UCR Institute for the Development of Educational Applications

 Editor in Chief, Physiological and Biochemical Zoology

 Fail Lab: Episode One
 http://testtube.com/faillab/zoochosis-episode-one-evolution
 http://www.youtube.com/watch?v=c0msBWyTzU0

 
 From: R-sig-phylo [r-sig-phylo-boun...@r-project.org] on behalf of
 Solomon Chak [tc...@vims.edu]
 Sent: Thursday, July 16, 2015 3:31 AM
 To: r-sig-phylo@r-project.org
 Subject: [R-sig-phylo] PGLS with non-ultrametric tree

 Dear all,

 What are the pros and cons of the two methods to do pgls with a
 non-ultrametric tree? Many thanks!

 1) Convert the additive tree to ultrametric using penalized likelihood
 (Sanderson 2002) with ape:: chronopl after cross-validation to find the
 best lambda.

 2) Use weighted least-squares in which gls(y ~ x,
 correlation=corPagel(value=0.1, phy=phy, fixed=F),
 weights=varFixed(~tip.heights),
 data=dat)

 Cheers,
 Solomon Chak

 ---
 *Solomon **Tin Chi **Chak*
 Ph.D. Candidate
 Tel: (804) 684-7484
 Marine Biodiversity Laboratory
 
 http://www.vims.edu/research/units/labgroups/marine_biodiversity/index.php
 
 Virginia Institute of Marine Science http://www.vims.edu/
 College of William and Mary http://www.wm.edu/
 PO Box 1346 / Rt. 1208 Greate Rd., Gloucester Pt, VA 23062, USA

 [[alternative HTML version deleted]]

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 http://www.mail-archive.com/r-sig-phylo@r-project.org/


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Re: [R-sig-phylo] PGLS with non-ultrametric tree

2015-07-16 Thread Paolo Piras
I really think this post could be useful for the discussion

https://stat.ethz.ch/pipermail/r-sig-phylo/2010-September/000773.html

best
paolo


Da: R-sig-phylo r-sig-phylo-boun...@r-project.org per conto di Solomon Chak 
tc...@vims.edu
Inviato: giovedì 16 luglio 2015 23.19
A: Theodore Garland Jr
Cc: r-sig-phylo@r-project.org
Oggetto: Re: [R-sig-phylo] PGLS with non-ultrametric tree

Hi Dr. Garland,

I got the impression that an ultrametric tree is needed for comparative
analysis from this book http://www.springer.com/us/book/9783662435496ch.
2 pg. 38: most comparative analyses assume that the tree is ultrametric,
as the majority of analyses deal with evolution of phenotypic traits of
extant species with the underlying assumption is that the time available
for phenotypic evolution is the same for all taxa.

I understand that the methodology of PGLS can use any tree form, I suppose
there're different assumptions if I used an additive tree or an ultrametric
tree. Could you guide me to some references that explicitly discuss about
this?

Is it necessary to use an ultrametric tree If I want to estimate lambda in
the residual error of the gls and do a branch length transformation as Revell
2010
http://onlinelibrary.wiley.com/doi/10./j.2041-210X.2010.00044.x/pdf
suggested?

Many thanks!

Cheers,
Solomon Chak

---
*Solomon **Tin Chi **Chak*
Ph.D. Candidate
Tel: (804) 684-7484
Marine Biodiversity Laboratory
http://www.vims.edu/research/units/labgroups/marine_biodiversity/index.php
Virginia Institute of Marine Science http://www.vims.edu/
College of William and Mary http://www.wm.edu/
PO Box 1346 / Rt. 1208 Greate Rd., Gloucester Pt, VA 23062, USA


On Thu, Jul 16, 2015 at 2:55 PM, Theodore Garland Jr 
theodore.garl...@ucr.edu wrote:

 Dear Solomon,

 I don;t know where you are trying to go with this, but any proper
 implementation of PGLS (I am not talking about methods that transform the
 branch lengths with things like Grafen's rho, Pagel's lambda or OU models)
 should be able to use any tree of any shape.  This is the same as for
 phylogenetically independent contrasts.

 Cheers,
 Ted

 Theodore Garland, Jr., Professor
 Department of Biology
 University of California, Riverside
 Riverside, CA 92521
 Office Phone:  (951) 827-3524
 Facsimile:  (951) 827-4286 (not confidential)
 Email:  tgarl...@ucr.edu
 http://www.biology.ucr.edu/people/faculty/Garland.html
 http://scholar.google.com/citations?hl=enuser=iSSbrhwJ

 Director, UCR Institute for the Development of Educational Applications

 Editor in Chief, Physiological and Biochemical Zoology

 Fail Lab: Episode One
 http://testtube.com/faillab/zoochosis-episode-one-evolution
 http://www.youtube.com/watch?v=c0msBWyTzU0

 
 From: R-sig-phylo [r-sig-phylo-boun...@r-project.org] on behalf of
 Solomon Chak [tc...@vims.edu]
 Sent: Thursday, July 16, 2015 3:31 AM
 To: r-sig-phylo@r-project.org
 Subject: [R-sig-phylo] PGLS with non-ultrametric tree

 Dear all,

 What are the pros and cons of the two methods to do pgls with a
 non-ultrametric tree? Many thanks!

 1) Convert the additive tree to ultrametric using penalized likelihood
 (Sanderson 2002) with ape:: chronopl after cross-validation to find the
 best lambda.

 2) Use weighted least-squares in which gls(y ~ x,
 correlation=corPagel(value=0.1, phy=phy, fixed=F),
 weights=varFixed(~tip.heights),
 data=dat)

 Cheers,
 Solomon Chak

 ---
 *Solomon **Tin Chi **Chak*
 Ph.D. Candidate
 Tel: (804) 684-7484
 Marine Biodiversity Laboratory
 
 http://www.vims.edu/research/units/labgroups/marine_biodiversity/index.php
 
 Virginia Institute of Marine Science http://www.vims.edu/
 College of William and Mary http://www.wm.edu/
 PO Box 1346 / Rt. 1208 Greate Rd., Gloucester Pt, VA 23062, USA

 [[alternative HTML version deleted]]

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 Searchable archive at
 http://www.mail-archive.com/r-sig-phylo@r-project.org/


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Re: [R-sig-phylo] PGLS with non-ultrametric tree

2015-07-16 Thread David Bapst
Hi Solomon,

Comparatives methods are routinely applied to non-ultrametric trees,
contrary to the text you quoted. See the last chapter of that book.
The phylogeny-based analyses that do generally require ultrametric
trees are those that fit some form of a lineage diversification model,
because these models are ultimately descended from the idea of
treating the age of modern lineages as a reverse survivorship curve.
Most trait-based analyses are fine.

What does typically need to be done for at least some PGLS functions,
is accounting for unequal tip variances with non-ultrametric trees. I
usually do something like this, just using gls() and ape functions...

tiph - diag( vcv.phylo(tree) )
gls(X1~X2 ,correlation=corBrownian(value=1, phy=tree),
weights=varFixed(~tiph), data=data)

Cheers,
-Dave

On Thu, Jul 16, 2015 at 3:19 PM, Solomon Chak tc...@vims.edu wrote:
 Hi Dr. Garland,

 I got the impression that an ultrametric tree is needed for comparative
 analysis from this book http://www.springer.com/us/book/9783662435496ch.
 2 pg. 38: most comparative analyses assume that the tree is ultrametric,
 as the majority of analyses deal with evolution of phenotypic traits of
 extant species with the underlying assumption is that the time available
 for phenotypic evolution is the same for all taxa.

 I understand that the methodology of PGLS can use any tree form, I suppose
 there're different assumptions if I used an additive tree or an ultrametric
 tree. Could you guide me to some references that explicitly discuss about
 this?

 Is it necessary to use an ultrametric tree If I want to estimate lambda in
 the residual error of the gls and do a branch length transformation as Revell
 2010
 http://onlinelibrary.wiley.com/doi/10./j.2041-210X.2010.00044.x/pdf
 suggested?

 Many thanks!

 Cheers,
 Solomon Chak

 ---
 *Solomon **Tin Chi **Chak*
 Ph.D. Candidate
 Tel: (804) 684-7484
 Marine Biodiversity Laboratory
 http://www.vims.edu/research/units/labgroups/marine_biodiversity/index.php
 Virginia Institute of Marine Science http://www.vims.edu/
 College of William and Mary http://www.wm.edu/
 PO Box 1346 / Rt. 1208 Greate Rd., Gloucester Pt, VA 23062, USA


 On Thu, Jul 16, 2015 at 2:55 PM, Theodore Garland Jr 
 theodore.garl...@ucr.edu wrote:

 Dear Solomon,

 I don;t know where you are trying to go with this, but any proper
 implementation of PGLS (I am not talking about methods that transform the
 branch lengths with things like Grafen's rho, Pagel's lambda or OU models)
 should be able to use any tree of any shape.  This is the same as for
 phylogenetically independent contrasts.

 Cheers,
 Ted

 Theodore Garland, Jr., Professor
 Department of Biology
 University of California, Riverside
 Riverside, CA 92521
 Office Phone:  (951) 827-3524
 Facsimile:  (951) 827-4286 (not confidential)
 Email:  tgarl...@ucr.edu
 http://www.biology.ucr.edu/people/faculty/Garland.html
 http://scholar.google.com/citations?hl=enuser=iSSbrhwJ

 Director, UCR Institute for the Development of Educational Applications

 Editor in Chief, Physiological and Biochemical Zoology

 Fail Lab: Episode One
 http://testtube.com/faillab/zoochosis-episode-one-evolution
 http://www.youtube.com/watch?v=c0msBWyTzU0

 
 From: R-sig-phylo [r-sig-phylo-boun...@r-project.org] on behalf of
 Solomon Chak [tc...@vims.edu]
 Sent: Thursday, July 16, 2015 3:31 AM
 To: r-sig-phylo@r-project.org
 Subject: [R-sig-phylo] PGLS with non-ultrametric tree

 Dear all,

 What are the pros and cons of the two methods to do pgls with a
 non-ultrametric tree? Many thanks!

 1) Convert the additive tree to ultrametric using penalized likelihood
 (Sanderson 2002) with ape:: chronopl after cross-validation to find the
 best lambda.

 2) Use weighted least-squares in which gls(y ~ x,
 correlation=corPagel(value=0.1, phy=phy, fixed=F),
 weights=varFixed(~tip.heights),
 data=dat)

 Cheers,
 Solomon Chak

 ---
 *Solomon **Tin Chi **Chak*
 Ph.D. Candidate
 Tel: (804) 684-7484
 Marine Biodiversity Laboratory
 
 http://www.vims.edu/research/units/labgroups/marine_biodiversity/index.php
 
 Virginia Institute of Marine Science http://www.vims.edu/
 College of William and Mary http://www.wm.edu/
 PO Box 1346 / Rt. 1208 Greate Rd., Gloucester Pt, VA 23062, USA

 [[alternative HTML version deleted]]

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-- 
David W. Bapst, PhD
Adjunct Asst

Re: [R-sig-phylo] PGLS with non-ultrametric tree

2015-07-16 Thread Theodore Garland Jr
Yep, we've all been down this road before, but I'm too old to remember!

Thanks and cheers,
Ted

From: Paolo Piras [paolo.pi...@uniroma3.it]
Sent: Thursday, July 16, 2015 2:38 PM
To: Solomon Chak; Theodore Garland Jr
Cc: r-sig-phylo@r-project.org
Subject: Re: [R-sig-phylo] PGLS with non-ultrametric tree

I really think this post could be useful for the discussion

https://stat.ethz.ch/pipermail/r-sig-phylo/2010-September/000773.html

best
paolo


Da: R-sig-phylo r-sig-phylo-boun...@r-project.org per conto di Solomon Chak 
tc...@vims.edu
Inviato: giovedì 16 luglio 2015 23.19
A: Theodore Garland Jr
Cc: r-sig-phylo@r-project.org
Oggetto: Re: [R-sig-phylo] PGLS with non-ultrametric tree

Hi Dr. Garland,

I got the impression that an ultrametric tree is needed for comparative
analysis from this book http://www.springer.com/us/book/9783662435496ch.
2 pg. 38: most comparative analyses assume that the tree is ultrametric,
as the majority of analyses deal with evolution of phenotypic traits of
extant species with the underlying assumption is that the time available
for phenotypic evolution is the same for all taxa.

I understand that the methodology of PGLS can use any tree form, I suppose
there're different assumptions if I used an additive tree or an ultrametric
tree. Could you guide me to some references that explicitly discuss about
this?

Is it necessary to use an ultrametric tree If I want to estimate lambda in
the residual error of the gls and do a branch length transformation as Revell
2010
http://onlinelibrary.wiley.com/doi/10./j.2041-210X.2010.00044.x/pdf
suggested?

Many thanks!

Cheers,
Solomon Chak

---
*Solomon **Tin Chi **Chak*
Ph.D. Candidate
Tel: (804) 684-7484
Marine Biodiversity Laboratory
http://www.vims.edu/research/units/labgroups/marine_biodiversity/index.php
Virginia Institute of Marine Science http://www.vims.edu/
College of William and Mary http://www.wm.edu/
PO Box 1346 / Rt. 1208 Greate Rd., Gloucester Pt, VA 23062, USA


On Thu, Jul 16, 2015 at 2:55 PM, Theodore Garland Jr 
theodore.garl...@ucr.edu wrote:

 Dear Solomon,

 I don;t know where you are trying to go with this, but any proper
 implementation of PGLS (I am not talking about methods that transform the
 branch lengths with things like Grafen's rho, Pagel's lambda or OU models)
 should be able to use any tree of any shape.  This is the same as for
 phylogenetically independent contrasts.

 Cheers,
 Ted

 Theodore Garland, Jr., Professor
 Department of Biology
 University of California, Riverside
 Riverside, CA 92521
 Office Phone:  (951) 827-3524
 Facsimile:  (951) 827-4286 (not confidential)
 Email:  tgarl...@ucr.edu
 http://www.biology.ucr.edu/people/faculty/Garland.html
 http://scholar.google.com/citations?hl=enuser=iSSbrhwJ

 Director, UCR Institute for the Development of Educational Applications

 Editor in Chief, Physiological and Biochemical Zoology

 Fail Lab: Episode One
 http://testtube.com/faillab/zoochosis-episode-one-evolution
 http://www.youtube.com/watch?v=c0msBWyTzU0

 
 From: R-sig-phylo [r-sig-phylo-boun...@r-project.org] on behalf of
 Solomon Chak [tc...@vims.edu]
 Sent: Thursday, July 16, 2015 3:31 AM
 To: r-sig-phylo@r-project.org
 Subject: [R-sig-phylo] PGLS with non-ultrametric tree

 Dear all,

 What are the pros and cons of the two methods to do pgls with a
 non-ultrametric tree? Many thanks!

 1) Convert the additive tree to ultrametric using penalized likelihood
 (Sanderson 2002) with ape:: chronopl after cross-validation to find the
 best lambda.

 2) Use weighted least-squares in which gls(y ~ x,
 correlation=corPagel(value=0.1, phy=phy, fixed=F),
 weights=varFixed(~tip.heights),
 data=dat)

 Cheers,
 Solomon Chak

 ---
 *Solomon **Tin Chi **Chak*
 Ph.D. Candidate
 Tel: (804) 684-7484
 Marine Biodiversity Laboratory
 
 http://www.vims.edu/research/units/labgroups/marine_biodiversity/index.php
 
 Virginia Institute of Marine Science http://www.vims.edu/
 College of William and Mary http://www.wm.edu/
 PO Box 1346 / Rt. 1208 Greate Rd., Gloucester Pt, VA 23062, USA

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