CSIMPORTANT COMMENT; Liposomal Encapsulation

[Brooks Bradley]

Several list members have experienced difficulty in obtaining reliable, accurate indications of the actual encapsulation percentages..using the Do It Yourself technique which I outlined in an earlier post.  NOTE: The methodology outlined IS NOT RELIABLE as a true indication of achieved encapsulation levels of vitamin C.  The ONLY truly reliable means of, accurately, knowing the degree of encapsulation is via Electron Microscopic
observation.  This entails preparation protocols/equipment  beyond means of most personseven most laboratories.  
 In my enthusiasm to avail list members of a "SIMPLE" protocol, which had yielded results reliable enough to validate useful parametersI outlined a test which is
NOT TRULY INDICATIVE of the encapsulated Vitamin C content.  
  For those of you who followed my instructions for making a general determination of the approximate, percentage levels of encapsulation, the discrepancy in resultsyou may have achieved, ARE NOT YOUR FAULT and did not result from a "broken protocol" on your part.
   Partial Explanation:  There are just too many variables involved (volumes/strengths/ratios) of the reactive materials, especially bicarbonate and ascorbic acid fractions presenting. Just varying the velocity of mixing can result in "marked" differences of the gas-release reaction.  The gas generation is NOT a true reflection of the percentage of non-encapsulated Vitamin C fraction.
While my intentions were of the highest order, my information was incomplete and poorly constructed.  For this, I deeply apologize.
Although some may be able to replicate my results, the protocol proper, is
too unreliable for general application.
However, all is not lost.  There is a VISIBLE, indicator of the general
success of the experimenter's procedure.  We HAVE determined, via electron microscope examination by an associated laboratorythat almost any solution achieved using the simple procedures outlined in my original post for producing encapsulation.yields
>50% encapsulation.  One excellent indicator is the degree of "apparent homogenization".
That is, the uniform, milky, appearance..and its "long-term" (days) retention.
 I have no defensible excuse for my errortherefore, must appeal
to the graces of the list membersfor understanding.
 Sincerely,  Brooks Bradley.
P.S.  I would be remiss if I failed to encourage ALL OF YOU to continue your researches
addressing use of this most promising protocol for encapsulation.  The majority of our
investigations have yielded VERY powerful positive results. 


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Re: CSIMPORTANT COMMENT; Liposomal Encapsulation

[Bernadette]

Thank you for explaining, Brooks.   The end result of my encapsulated Vitamin C 
liquid has been a milky homogenization from what I started with.  I think this 
is then perfect for our daily consumption.

Bernadette
  - Original Message - 
  From: Brooks Bradley 
  To: Silver-list@eskimo.com 
  Sent: Thursday, August 27, 2009 11:07 AM
  Subject: CSIMPORTANT COMMENT; Liposomal Encapsulation


  Several list members have experienced difficulty in obtaining reliable, 
accurate indications of the actual encapsulation percentages..using the Do 
It Yourself technique 

Re: CSIMPORTANT COMMENT; Liposomal Encapsulation

[Gail Rice]

I tried the protocol again - this time with a new Ultrasonic cleaner. It
worked beautifully! There was no visible separation after sitting overnight.
I did the baking soda test because I hadn't read Brooks's email yet and just
by luck, it barely foamed at all, so I was satisfied and drank it
immediately.
I repeated the protocol (after reading this newer post) in the original
ultrasonic cleaner but sure enough, although the mixture looked
encouragingly milky, it began to separate within a minute and by about 10
minutes has a thick dark yellow layer on the bottom. So, I don't know why
this first U S cleaner does less well, but it does. So, I'll use the
replacement cleaner and make the excellent encapsulated Vitamin C. I don't
understand how these machines actually work or why this would happen.  -Gail

Re: CSIMPORTANT COMMENT; Liposomal Encapsulation

[Ruth Bertella]

What is the make/model of the cleaner you used that worked?

Thanks!
Ruth
  - Original Message - 
  From: Gail Rice 
  To: silver-list@eskimo.com 
  Sent: Thursday, August 27, 2009 12:45 PM
  Subject: Re: CSIMPORTANT COMMENT; Liposomal Encapsulation


  I tried the protocol again - this time with a new Ultrasonic cleaner. It 
worked beautifully! There was no visible separation after sitting overnight. I 
did the baking soda test because I hadn't read Brooks's email yet and just by 
luck, it barely foamed at all, so I was satisfied and drank it immediately.
  I repeated the protocol (after reading this newer post) in the original 
ultrasonic cleaner but sure enough, although the mixture looked encouragingly 
milky, it began to separate within a minute and by about 10 minutes has a thick 
dark yellow layer on the bottom. So, I don't know why this first U S cleaner 
does less well, but it does. So, I'll use the replacement cleaner and make the 
excellent encapsulated Vitamin C. I don't understand how these machines 
actually work or why this would happen.  -Gail

Re: CSIMPORTANT COMMENT; Liposomal Encapsulation

[paul catania]

Gail,
Do you have the smaller UC or the larger? Also, how much did you fill up the 
tank, in reference to the max and min lines?
Paul
  - Original Message - 
  From: Gail Rice 
  To: silver-list@eskimo.com 
  Sent: Thursday, August 27, 2009 10:45 AM
  Subject: Re: CSIMPORTANT COMMENT; Liposomal Encapsulation


  I tried the protocol again - this time with a new Ultrasonic cleaner. It 
worked beautifully! There was no visible separation after sitting overnight. I 
did the baking soda test because I hadn't read Brooks's email yet and just by 
luck, it barely foamed at all, so I was satisfied and drank it immediately.
  I repeated the protocol (after reading this newer post) in the original 
ultrasonic cleaner but sure enough, although the mixture looked encouragingly 
milky, it began to separate within a minute and by about 10 minutes has a thick 
dark yellow layer on the bottom. So, I don't know why this first U S cleaner 
does less well, but it does. So, I'll use the replacement cleaner and make the 
excellent encapsulated Vitamin C. I don't understand how these machines 
actually work or why this would happen.  -Gail

RE: CSIMPORTANT COMMENT; Liposomal Encapsulation

[Dianne France]

Brooks,

I appreciate very much your information and plan on trying the encapsulation 
myself as soon as I get equipment.  I have bought the other vit C and am happy 
with results from it.  If you had not posted most of us wouldn't know of the 
encapsulated Lip. Vit C.  Thank you for thinking of us.  I have a cousin with 
cancer that I have forwarded ordering information to.  I know he would not try 
to make it himself.  

 

Dianne
 


From: brooks76...@lycos.com
To: Silver-list@eskimo.com
Date: Thu, 27 Aug 2009 11:07:31 -0400
Subject: CSIMPORTANT COMMENT; Liposomal Encapsulation

Several list members have experienced difficulty in obtaining reliable, 
accurate indications of the actual encapsulation percentages..using the Do 
It Yourself technique which I outlined in an earlier post. NOTE: The 
methodology outlined IS NOT RELIABLE as a true indication of achieved 
encapsulation levels of vitamin C. The ONLY truly reliable means of, 
accurately, knowing the degree of encapsulation is via Electron Microscopic 
observation. This entails preparation protocols/equipment beyond means of most 
personseven most laboratories. 
In my enthusiasm to avail list members of a SIMPLE protocol, which had 
yielded results reliable enough to validate useful parametersI outlined a 
test which is 
NOT TRULY INDICATIVE of the encapsulated Vitamin C content. 
For those of you who followed my instructions for making a general 
determination of the approximate, percentage levels of encapsulation, the 
discrepancy in resultsyou may have achieved, ARE NOT YOUR FAULT and did not 
result from a broken protocol on your part. 
Partial Explanation: There are just too many variables involved 
(volumes/strengths/ratios) of the reactive materials, especially bicarbonate 
and ascorbic acid fractions presenting. Just varying the velocity of mixing can 
result in marked differences of the gas-release reaction. The gas generation 
is NOT a true reflection of the percentage of non-encapsulated Vitamin C 
fraction. 
While my intentions were of the highest order, my information was incomplete 
and poorly constructed. For this, I deeply apologize. 
Although some may be able to replicate my results, the protocol proper, is 
too unreliable for general application. 
However, all is not lost. There is a VISIBLE, indicator of the general 
success of the experimenter's procedure. We HAVE determined, via electron 
microscope examination by an associated laboratorythat almost any solution 
achieved using the simple procedures outlined in my original post for producing 
encapsulation.yields 
50% encapsulation. One excellent indicator is the degree of apparent 
homogenization. 
That is, the uniform, milky, appearance..and its long-term (days) 
retention. 
I have no defensible excuse for my errortherefore, must appeal 
to the graces of the list membersfor understanding. 
Sincerely, Brooks Bradley. 
P.S. I would be remiss if I failed to encourage ALL OF YOU to continue your 
researches 
addressing use of this most promising protocol for encapsulation. The majority 
of our 
investigations have yielded VERY powerful positive results. -- The Silver List 
is a moderated forum for discussing Colloidal Silver. Instructions for 
unsubscribing are posted at: http://silverlist.org To post, address your 
message to: silver-list@eskimo.com Address Off-Topic messages to: 
silver-off-topic-l...@eskimo.com The Silver List and Off Topic List archives 
are currently down... List maintainer: Mike Devour
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