RE: CSExtraction Methods for Peperine
Is it possible to make with without using the microwave? Can you use a toaster oven or regular oven? Dianne Date: Tue, 29 Sep 2009 17:18:21 -0500 From: stephen.nor...@ngc.com To: silver-list@eskimo.com Subject: CSExtraction Methods for Peperine Just FYI. Some surprisingly easy methods for extracting Peperine from black pepper from http://www.curevents.com/vb/showthread.php?t=25598. - Steve N Black pepper, by itself, is not a very efficient way to increase the uptake of curcumin. Piperine makes up only about 3% mass (dry) of the pepper. The breakdown of the pepper material will also cause a lag in piperine availability. Extraction is neccessary. I have a great deal of experience extracting piperine using househould items and have 27g of pure yellow crystals sitting on my desk at this moment. Here is how I do it. Microwave(*1) ~100g of crushed black pepper spread out on a plate; make sure you have a small glass of water also in the microwave to prevent feedback to the magnetron. Shake this microwaved pepper in a mason jar with either denatured alcohol from a harware store or anhydrous isopropyl alcohol(*2) (IPA, rubbing alcohol) for about 10 minutes. You now need to filter out the solids from this mix. I usually make a two stage filter out of the top half of a 1 liter water bottle, a generic cooking funnel, and a few coffee filters. http://img468.imageshack.us/img468/5346/filtersetup1rt.gif Once this finishes filtering you can then evaporate the solvents off the piperine by pouring the solution into a pan with a large surface area and very gently heating it (I sometimes just let it sit without heat for a day or so when lazy). This should leave you with a sticky yellow crystaline substance with bits of brown gel mixed in (this brown is chavacine, responsible for the smell of pepper). Scrape this mix up and disolve in acetone, filter again, then recrystalize by either evaporating off the solvent or dumping the solution into a liter or two of ice cold water. This should leave you with fairly pure piperine. (1) Microwaving black pepper in a non-polar solvent (or by itself) will tend to cause cell rupture allowing for easier extraction of piperine. http://rapidshare.de/files/6610631/Microwave_assisted_extraction_of_piperine.pdf.html (2) You can make dry IPA fairly easily. Buy epsom salts from a grocery store and heat in the oven for ~15 minutes to drive off moisture. Put an appreciable amount of now dry epsom salts into the rubbing alcohol and shake like mad for a minute. Filter the mix and you should have pure IPA without water. It's best to start with 91% IPA if possible but 70% will do. _ Lauren found her dream laptop. Find the PC that’s right for you. http://www.microsoft.com/windows/choosepc/?ocid=ftp_val_wl_290
Re: CSExtraction Methods for Peperine
I haven't tried it but I don't know why not. - Steve N From: Dianne France dianne_fra...@hotmail.com To: silver-list silver-list@eskimo.com Sent: Wed Sep 30 01:44:51 2009 Subject: RE: CSExtraction Methods for Peperine Is it possible to make with without using the microwave? Can you use a toaster oven or regular oven? Dianne Date: Tue, 29 Sep 2009 17:18:21 -0500 From: stephen.nor...@ngc.com To: silver-list@eskimo.com Subject: CSExtraction Methods for Peperine Just FYI. Some surprisingly easy methods for extracting Peperine from black pepper from http://www.curevents.com/vb/showthread.php?t=25598 http://www.curevents.com/vb/showthread.php?t=25598 . - Steve N Black pepper, by itself, is not a very efficient way to increase the uptake of curcumin. Piperine makes up only about 3% mass (dry) of the pepper. The breakdown of the pepper material will also cause a lag in piperine availability. Extraction is neccessary. I have a great deal of experience extracting piperine using househould items and have 27g of pure yellow crystals sitting on my desk at this moment. Here is how I do it. Microwave(*1) ~100g of crushed black pepper spread out on a plate; make sure you have a small glass of water also in the microwave to prevent feedback to the magnetron. Shake this microwaved pepper in a mason jar with either denatured alcohol from a harware store or anhydrous isopropyl alcohol(*2) (IPA, rubbing alcohol) for about 10 minutes. You now need to filter out the solids from this mix. I usually make a two stage filter out of the top half of a 1 liter water bottle, a generic cooking funnel, and a few coffee filters. http://img468.imageshack.us/img468/5346/filtersetup1rt.gif http://img468.imageshack.us/img468/5346/filtersetup1rt.gif Once this finishes filtering you can then evaporate the solvents off the piperine by pouring the solution into a pan with a large surface area and very gently heating it (I sometimes just let it sit without heat for a day or so when lazy). This should leave you with a sticky yellow crystaline substance with bits of brown gel mixed in (this brown is chavacine, responsible for the smell of pepper). Scrape this mix up and disolve in acetone, filter again, then recrystalize by either evaporating off the solvent or dumping the solution into a liter or two of ice cold water. This should leave you with fairly pure piperine. (1) Microwaving black pepper in a non-polar solvent (or by itself) will tend to cause cell rupture allowing for easier extraction of piperine. http://rapidshare.de/files/6610631/Microwave_assisted_extraction_of_piperine.pdf.html http://rapidshare.de/files/6610631/Microwave_assisted_extraction_of_piperine.pdf.html (2) You can make dry IPA fairly easily. Buy epsom salts from a grocery store and heat in the oven for ~15 minutes to drive off moisture. Put an appreciable amount of now dry epsom salts into the rubbing alcohol and shake like mad for a minute. Filter the mix and you should have pure IPA without water. It's best to start with 91% IPA if possible but 70% will do. Lauren found her dream laptop. Find the PC that’s right for you. http://www.microsoft.com/windows/choosepc/?ocid=ftp_val_wl_290
