Re: [aroma.affymetrix] exon array analysis errors

2009-11-24 Thread Mark Robinson
Hi Yu Chuan. Comments below. On 24-Nov-09, at 1:00 PM, Yu Chuan wrote: Hi, I am pre-processing 8 exon arrays (Hu-Ex-1_0-st-v2) and doing quality assessment. When I plotted the NUSE using plotNUSE, I found that the y- axis limit is too wide, such that the boxplots were all squeezed

Re: [aroma.affymetrix] how to analysis the FIRMA score

2009-11-24 Thread Mark Robinson
Hi Jiang. A couple quick comments below. On 20-Nov-09, at 10:07 AM, camelbbs wrote: Hi, After I got the firma scores, how can i analyze it. I see boxplot of firma scores in the paper. So how i can get the same result. You can use the boxplot() command on your matrix of FIRMA scores?

[aroma.affymetrix] Re: exon array analysis errors

2009-11-24 Thread Yu Chuan
Hi Mark, Thanks for your prompt reply. Below is my complete R code together with error information. I tried what you suggested, but the resulted NUSE plot still looks the same. Also, what does it mean if the boxplot for a chip's exon-level NUSE suggests that this chip maybe an outlier (i.e. the

[aroma.affymetrix] Re: exon array analysis errors

2009-11-24 Thread Yu Chuan
Mark, I think the below info. may help too. Looks like all the gene-level NUSE are 0. How could this happen? z - plotNuse(qamTr) z $`20091119_Colon4_Exon2` $`20091119_Colon4_Exon2`$stats [1] 0 0 0 0 0 $`20091119_Colon4_Exon2`$n [1] 18705 $`20091119_Colon4_Exon2`$conf [1] 0 0