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SYNCHROTRON BEAM TIME FOR MACROMOLECULAR CRYSTALLOGRAPHY
AT THE UNDULATOR BEAMLINE X06SA AT SLS FROM MAY - AUGUST 2007
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- PILATUS 6M pixel detector will become ava
Dear all, its easy and paperless, so get your proposals in for the next
round of beamtime at bm14. See links below for submitting your proposals
SYNCHROTRON BEAM TIME FOR MACROMOLECULAR CRYSTALLOGRAPHY
AT THE UK/EMBL MAD
Dear All,
Maybe this is a trivial question:
My data should have 6 molecules in one assymetric unit. MR could find out
4 molecules. After this, no matter how hard I have tried, no more
molecules can be found. At this stage, I suppose that all other copies are
dis-ordered. And go ahead to do r
Hi,
What software is recommended for building structures from NMR data?
Cheers,
Dan.
Dear all
I am trying MR in molrep(CCP4) with P21212.mtz.I have tried nearly all space
group.Rfact and corr.coeff is 57-59% and 23-25% resp.what should I try to
lower the Rfact.The data is at 2.2A.Any suggestions are welcome.Thanx in
advance.
Shivesh
Is the result clearly better in one space group than all the others?
ie Are you sure you have a correct solution?
Eleanor
shivesh kumar wrote:
Dear all
I am trying MR in molrep(CCP4) with P21212.mtz.I have tried nearly all
space group.Rfact and corr.coeff is 57-59% and 23-25% resp.what shoul
This is merely a second posting of a message from about 10 days ago -
there is no need to reply if you have already expressed interest
Sometimes this sort of disorder is due to an error , so the first thing
is to check very carefully that the solution makes sense.
Why are you so sure there are 6 copies in the asymmetric unit?
In situations like this I first worry about SG.
Is there a pseudo-translation vector? This can make i
Assuming that the MR solution is correct (I agree with Eleanor, that you
need to be certain about your selection of space group), then use your
complete model of chain A superimposed on chains B, C, and D. Then refine
and build the heck out of it. In a similar situation, it was relatively
easy for
> software is recommended for building structures
Actually, I can recommend some HARDWARE:
It is called 'X-ray diffractometer'.
Cheers, BR
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Dan
Bolser
Sent: Tuesday, February 13, 2007 4:54 AM
To: CCP4BB@J
Dear all,
I need to decide between buying an AKTA prime and an AKTA FPLC from GE health
care. I understand AKTA prime is a low-pressure system, but because it is too
much cheaper than AKTA FPLC, it is still very attractive to me.
I will mainly use it for Nickel columns and gel filtration colum
Sorry I forget to tell ya the details:
The SG of my Data is cubic P4332. Cell is 251.34A in all three dimensions.
Resolution is 3.1A. 5,6,7,8 might be the possible copies from Matthew
coeficient. But I just trust 6 because Chinese like the number 6
(happy Chinese new year by the way :)). No pseud
Hi,
Do you have any biochemical evidence that points to oligomeric nature
of your protein? Does SEC indicate monomer/dimer/trimer/tetramer?
If not or even if so, what does the crystal packing with your MR
solution with 4 molecules suggest about monomer interactions? Does that
correlate with S
Are you sure that you want AKTA anything? They are so expensive.
We have had good service from the old style ("HR") Bio-Rad Bio-Logic
chromatography systems. The new ones are called "DuoFlow".
-Dan
On Tue, 13 Feb 2007, Frank Lee wrote:
>
> Dear all,
>
> I need to decide between buying an AKTA p
Dear Frank
AKTAs are excellent chromatographic systems, but you must know that AKTA
prime is not the best choice if you want to do "serious" chromatography.
AKTA prime has only one pump and a valve that alternatively extract
solvent from the two different reservoirs. If you want to do a gradient,
Dear Frank
AKTAs are excellent chromatographic systems, but you must know that AKTA
prime is not the best choice if you want to do "serious" chromatography.
AKTA prime has only one pump and a valve that alternatively extract
solvent from the two different reservoirs. If you want to do a gradient,
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