I very much agree with Holton. I also find the following to be a simple
and very helpful argument in the discussion (it was given to me by Morten
Kjeldgaard):
Your model should reproduce your weak data by weak Fc's - therefore you
need your weak reflections in the refinement
I personally like
Hi Weijun and all,
Anyone tried the Stratagene's ArcticExpress^(TM) Competent Cells for
expression of proteins at low temperature (13 degree). The company
claims that the cells encode chaperonins Cpn60 and Cpn10 and they can
enhance the protein folding and solubility.
Hongmin
Dear All,
I
James Holton wrote:
I generally cut off integration at the shell wheree I/sigI 0.5 and
then cut off merged data where MnI/sd(I) ~ 1.5. It is always easier
to to cut off data later than to re-integrate it. I never look at
the Rmerge, Rsym, Rpim or Rwhatever in the highest resolution shell.
Hi Dima,
Can anyone comment on availability of Archaea expression systems?
Based on available structures, it would appear that their chaperons
are much more similar to eukaryotic one than anything present in
eubacteria. For a long time, I've been puzzling over why there is
still no
Hello,
I posted a message about this a month ago and thanks to everyone for
their responses. At the time, I did not fully appreciate the problem
I was dealing with so this time my question is much more specific. I
would very much appreciate your help as this structure is turning out
to
Peter,
I had a similar case, but the centering was pseudo-I in the
monoclinic system (Oksanen et al. (2006) Acta Cryst. D62 1369-1374).
I don't think you need to delete any reflections for MR; you should
have higher than normal correlation coefficients as well as R-values.
If you see
Dear Peter,
It seems to me that you are solving an incommensurately modulated structure,
since the pseudo-translation vector t = 0a + 0.47b + 0.5c having the b
component equal to 0.47 and not exactly 0.5. There should be satellite
reflections which are NOT on nodes of the reciprocal lattice
Dear Prof. Fan,
I do not think that this is a neccesarely an incommensurable structure. A lot
of structures have translational NCS close to a translational that would make
the space group higher and/or the primitive cell smaller.
If one has C2221 and one destroys the centring operation
