Wow! Those are two pretty amazing structures.
For those of you who haven't had a look, the ordered molecules are in
layers with *huge* gaps in between, much greater than in 2hr0.
And yet both of these structures were solved with experimental phasing
(SIRAS) unlike 2hr0, and the data is to
There is also one case of a protein structure that I am aware of, where a
similar problem has been tackled (the phenomenon is also known as
one-dimensional disorder, according to A.J.C. Wilson - yes, the one how
invented the plot).
Check: Trame, CB mcKay, DB (2001). Acta Cryst. D57,
Dear Todd,
Your diffraction pattern very much looks like suffering from a so-called
lattice-translocation defect (see e.g. acta cryst D57, 1079; D61, 67 and D61,
932). In this case, the diffuse spots are caused by stochastic discrete shifts
between successive layers. Since you do not seem to
This is just a gentle reminder - the deadline for the applications to
the M2M course is in 1 month.
=
We would like to announce the Practical Course on Training in methods
for Macromolecular Crystallography M2M-7: From Measurement to
A position is available immediately to work in the group of Dr John Rafferty at
the University of Sheffield for structural studies of proteins involved in DNA
recombination in Gram-positive organisms. The group focuses particularly on
those proteins that bind Holliday junctions and has
Further to the previous posting about a PDRA position in Sheffield, additional
details and the application procedure are available at
http://www.sheffield.ac.uk/jobs/research.html (post reference R05486).
With apologies for the omission.
Thanks
John
Dr J.B.Rafferty
Dept Molecular Biology
On Mon, 2007-08-27 at 19:34 +0200, [EMAIL PROTECTED] wrote:
I am trying to use the NCS Edits in Coot to be able to move a range of
residues from a molecule to the NCS related ones and the command
(copy-residue-range-from-ncs-master-to others ) is not working.
The fragment that I want to
Postdoctoral Scientist in X-ray structural analysis of plant proteins
A postdoctoral staff position is available at the Institute of
Biological Chemistry of the Technical University Munich starting from
November 2007 for initially three years. Projects are focused at the
structural study of
Hi CCP4BB,
Is there a straightforward way to compute the mean phase difference
between two phase columns in an MTZ file, allowing for the difference in
the origin? I am thinking something like:
Cad together two reflection files - renaming the PHIB column, most
likely
Compute phi offset - apply
For a full answer to all your questions, I refer you to the classic
textbook of M. M. Woolfson an introduction to x-ray crystallography by
Cambridge University Press. This book has been quite helpful to me of
late. Unlike some similar texts I find it easy to read. There are even
examples!
Yup. cphasematch
It gives unweighted and weighted mean phase errors and F and E
correlations. It'll also try the opposite hand if appropriate.
It's in the GUI under Clipper utilities.
For an example script, run it in the GUI and then view the command file.
Kevin
Winter, G (Graeme) wrote:
Hi,
I am working on a 60 kDa C. elegans protein that is predicted to be mostly
alpha-helix. It is over-expressed in E.coli and the yield is about 1 mg/L of
cell culture. The CD spec at 4 degree showed the presence of dominant
alpha-helix. However, we dont have any functional assay to
I got several suggestions.
I thought it is time to answer some questions, prepare a partial
quasi- summary and give some more feedback. Please, keep sending me any
more insights and suggestions you might have.
Briefly, the problem is:
P622 (no systematic abscences), strong spots at
Good summary as expected from James.
Have you ever heard of photon-photon scattering?
Well yes! See for example
http://2physics.blogspot.com/2006/03/photon-photon-scattering.html
which says according to Quantum Electrodynamics (QED), particles can still be
created in this emptiness of vacuum
Hi,
I have a protein that binds nucleotides, and in my structure, it
appears that the binding pocket is partial occupied by ADP and AMP;
the beta phosphate of ADP is transfered to another molecule. I want
to refine the structure with both ADP and AMP modeled in the sight and
manually vary their
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