But I cannot use this error to judge whether
something I do to the refinement is a significant improvement
or not. Indirect proof through argumentum absurdum:
Assume the su of Rfree is 0.008, and I build n waters. Rf drops
from 24.4 to 13.9, by 0.005, less than the su. I build n+m waters,
say
Dear All,
The closing date for applications to the Membrane Protein Lab (MPL) at Diamond
is approaching:
Please send in your application by the 27th of February, if you haven't already
done so.
The MPL is a research facility which provides equipment and expertise in the
area of membrane
As Edward MITCHELL suggested, I made a phased anomalous difference map
and could clearly see the S atom of my sulfide bridges and 1 Ca atom as
I espected. Concerning the unknown density, the shape is the same as in
the fo-fc map Updated images at :
Dear all
sorry if this query has already been asked. Whenever, I run a job for
molecular replacement in Molrep program using ccp4i, it fails to run and
shows the following error.
Child killed: segementation violation
I would appreciate for suggestions to fix this eror.
xishan
Remember that since it is on a special position (2-fold?), you may not
be looking at a single object, but an overlay from two or more
positions.
The anomalous map is very useful; the mystery molecule must have an
anomalous scatterer. It would be even more helpful if you had mentioned
the
There was once a fellow named Zeno who made a similar argument.
Aristotle was not convinced.
Dr. Merritt,
are you bringing a proof from Aristotle? But he believed in intelligent
design (see below for his argument), and therefore would be shunned and
blacklisted these days from any
Dear Xishan
Can you tell me what version of CCP4 you are running and on which
platform? Have you been able to run other programs through ccp4i
successfully?
Does the molrep job fail immediately or does it produce any output
before failing? If the latter, it would be very useful to see all the
Dear All,
I set up drops using RTISCC:1Lambda protein complex+1Lambda reservoir.
But I am not sure how much another ligand(50mM) I should add into the
drop. Any suggestion? Thank you very much.
Yanming
